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ZHENG Yaxian, ZHANG Wunan, HE Liping, WU Ruinan, SHAN Wei, LIU Min, HUANG Yuan. Trimethyl chitosan nanoparticles coated with Pluronic F-127 for oral insulin delivery system[J]. Journal of China Pharmaceutical University, 2016, 47(4): 442-447. DOI: 10.11665/j.issn.1000-5048.20160409
Citation: ZHENG Yaxian, ZHANG Wunan, HE Liping, WU Ruinan, SHAN Wei, LIU Min, HUANG Yuan. Trimethyl chitosan nanoparticles coated with Pluronic F-127 for oral insulin delivery system[J]. Journal of China Pharmaceutical University, 2016, 47(4): 442-447. DOI: 10.11665/j.issn.1000-5048.20160409

Trimethyl chitosan nanoparticles coated with Pluronic F-127 for oral insulin delivery system

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  • The purpose of this investigation was to develop Pluronic F-127 coated N-trimethyl chitosan nanoparticles(F-S NPs)of insulin as the model drug and asses their penetration of the mucosal barriers. Single factor screening was used to optimize the formulations of nanoparticles and the nanoparticles were characterized. Their particle size, Zeta potential, encapsulation efficiencies and drug loading were assayed to be(240. 6±6. 51)nm, (10. 42±1. 60)mV, (43. 39±2. 83)% and(3. 39±0. 57)%, respectively. The impact of PF-127 on mucin binding in vitro and nanoparticles′s transport in freshly obtained mucus were also evaluated. The mucin affinity of F-S NPs was significantly reduced when compared to that of the N-trimethyl chitosan nanoparticles(S NPs), i. e. , 28% of the latter. And F-S NPs was found to have an improved mucosal penetrating capability. Mucus-secreting HT29-MTX-E12(E12)cell monolayer was selected to investigate their cellular uptake. F-S NPs exhibited higher penetration coefficient than both free insulin and S NPs in mucus-secreting epithelium cells, i. e. , 16-fold and 1. 4-fold, respectively. Data suggest that F-S NPs be potential carriers to cross mucosal barriers and enhance the cellular uptake of insulin.
  • [1]
    Chin RL,Martinez R,Garmel G.Gas gangrene from subcutaneous insulin administration[J].Am J Emerg Med,1993,11(6):622-625.
    [2]
    Bakhru SH,Furtado S,Morello AP,et al.Oral delivery of proteins by biodegradable nanoparticles[J].Adv Drug Deliv Rev,2013,65(6):811-821.
    [3]
    Smith J,Wood E,Dornish M.Effect of chitosan on epithelial cell tight junctions[J].Pharm Res,2004,21(1):43-49.
    [4]
    Sonaje K,Lin KJ,Tseng MT,et al.Effects of chitosan-nanoparticle-mediated tight junction opening on the oral absorption of endotoxins[J].Biomaterials,2011,32(33):8712-8721.
    [5]
    Ensign LM,Cone R,Hanes J.Oral drug delivery with polymeric nanoparticles:the gastrointestinal mucus barriers[J].Adv Drug Deliv Rev,2012,64(6):557-570.
    [6]
    Cone RA.Barrier properties of mucus[J].Adv Drug Deliv Rev,2009,61(2):75-85.
    [7]
    Behrens I,Pena AIV,Alonso MJ,et al.Comparative uptake studies of bioadhesive and non-bioadhesive nanoparticles in human intestinal cell lines and rats:the effect of mucus on particle adsorption and transport[J].Pharm Res,2002,19(8):1185-1193.
    [8]
    Sadeghi AMM,Dorkoosh FA,Avadi MR,et al.Preparation,characterization and antibacterial activities of chitosan,N-trimethyl chitosan(TMC)and N-diethylmethyl chitosan(DEMC)nanoparticles loaded with insulin using both the ionotropic gelation and polyelectrolyte complexation methods[J].Int J Pharm,2008,355(1):299-306.
    [9]
    Yin L,Ding J,He C,et al.Drug permeability and mucoadhesion properties of thiolated trimethyl chitosan nanoparticles in oral insulin delivery[J].Biomaterials,2009,30(29):5691-5700.
    [10]
    Mao S,Germershaus O,Fischer D,et al.Uptake and transport of PEG-graft-trimethyl-chitosan copolymer-insulin nanocomplexes by epithelial cells[J].Pharm Res,2005,22(12):2058-2068.
    [11]
    Xie MF.Evaluation methods of comparability of dissolution curve[J].Chin J Pharm(中国医药工业杂志),2009,40(4):308-311.
    [12]
    Shan W,Zhu X,Liu M,et al.Overcoming the diffusion barrier of mucus and absorption barrier of epithelium by self-assembled nanoparticles for oral delivery of insulin[J].ACS Nano,2015,9(3):2345-2356.
    [13]
    Jin Y,Song Y,Zhu X,et al.Goblet cell-targeting nanoparticles for oral insulin delivery and the influence of mucus on insulin transport[J].Biomaterials,2012,33(5):1573-1582.
    [14]
    Lai SK,Wang YY,Hanes J.Mucus-penetrating nanoparticles for drug and gene delivery to mucosal tissues[J].Adv Drug Deliv Rev,2009,61(2):158-171.
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