Improvement of NET and 5-HTT expression involved in the anti-depressive effect of valproate

FEI Huizhi; HU Xiaoya; QIU Hongmei; LIU Dan; ZHOU Qixin

doi:10.11665/j.issn.1000-5048.20160414

Journal of China Pharmaceutical University > 2016 > 47(4): 474-478. > DOI: 10.11665/j.issn.1000-5048.20160414

FEI Huizhi, HU Xiaoya, QIU Hongmei, LIU Dan, ZHOU Qixin. Improvement of NET and 5-HTT expression involved in the anti-depressive effect of valproate[J]. Journal of China Pharmaceutical University, 2016, 47(4): 474-478. DOI: 10.11665/j.issn.1000-5048.20160414

Citation:

PDF (1215 KB)

Improvement of NET and 5-HTT expression involved in the anti-depressive effect of valproate

More Information

Graphical Abstract

Abstract

Abstract

This study aimed at investigating the correlations between antidepressive effect of valproate and improvements of NET and 5-HTT expression in depressive rats leaded by chronic mild unpredicted stress(CUMS)with solitary condition. Sixty male SD rats were divided into normal group(NG), model group(MG), valproate treated-normal group(VNG), and valproate treated-model group(VMG), randomly. The changes of depressive behaviors were evaluated by the open-field test and force swimming test. The contents of MDA, activities of SOD and CAT in serum, the mRNA and protein expression of NET, 5-HTT in hippocampus were determined by biochemical methods, Real-time PCR and Western Blot, respectively. Results showed that CUMS can significantly decrease the activities in open-field test, SOD and CAT activities in serum, expression of 5-HTT in hippocampus, and obviously increase the immobility time in force swimming test, the level of MDA and expression of NET. The treatment of valproate obviously improved the changes induced by CUMS. However, the treatment of valproate had no significant influences on behaviors of NG rats. So, it revealed that improvements of the mRNA and protein expression of NET, 5-HTT may be involved in the antidepressive effect of valproate.
- chronic mild unpredicted stress(CUMS),
- antidepressive,
- valproate,
- norepinephrine transporter,
- serotonin transporter

FullText(HTML)

References (15)

References

[1]	World Health Organization.Fact sheets N° 369:depression[EB/OL].(2015-10-01)[2016-01-04] .http://www.who.int/mediacentre/factsheets/fs369/en/.html.
[2]	Dedovic K,Duchesne A,Engert V,et al.Psychological,endocrine and neural responses to social evaluation in subclinical depression[J]. Soc Cogn Affect Neurosci,2014,9(10): 1632-1644.
[3]	Quesseveur G,Gardier AM,Guiard BP.The monoaminergic tripartite synapse: a putative target for currently available antidepressant drugs[J].Curr Drug Targets,2013,14(11):1277-1294.
[4]	Fornaro M,McCarthy MJ,DeBerardis D,et al.Adjunctive agomelatine therapy in the treatment of acute bipolar II depression:a preliminary open label study[J].Neuropsych Dis Treat,2013,9:243-251.
[5]	Xie JP,Ren ZB,Ma YC,et al.Comparing study on therapeutic of outpatients with agitated or anxiety depression by combination of SSRI and sodium valproate RX [J].Chin J Clin Pharm Ther(中国临床药理学与治疗学),2013,18(1):86-89.
[6]	Unterecker S,Reif A,Hempel S,et al.Interaction of valproic acid and the antidepressant drugs doxepin and venlafaxine:analysis of therapeutic drug monitoring data under naturalistic conditions[J].Int Clin Psychopharma,2014,29(4):206-211.
[7]	Maes M,Kubera M,Obuchowiczwa E,et al.Depression′s multiple comorbidities explained by(neuro)inflammatory and oxidative & nitrosative stress pathways[J].Neuro Endocrinol Lett,2011,32(1):7-24.
[8]	Hu XY,Li N,Fei HZ,et al.Anti-depressive effect of reboxetine on non-transporter inhibition in rats[J].Acta Acad Med Mil Tert(第三军医大学学报),2012,34(19):1977-1980.
[9]	Fei HZ,Wang H,Hu XY,et al. Improvement of oxidative stress and HPA axis function and elevation of BDNF expression involved in anti-depressive effect of paroxetine in rat model[J].Chin J Clin Pharm Ther(中国临床药理学与治疗学),2012,17(10):1137-1142.
[10]	Tang M,Lei JY,Sun X,et al.Stress-induced anhedonia correlates with lower hippocampal serotonin transporter protein expression[J].Brain Res,2013,1513:127-134.
[11]	Chen P, Fan Y, Li Y, et al. Chronic social defeat up-regulates expression of norepinephrine transporter in rat brains[J].Neurochem Int,2012,60(1):9-20.
[12]	Li N,Wang H,Wen W,et al.Improvement of oxidative/anti-oxidative stress balance and expression of NET and 5-HTT involve in the anti-depressive effect of reboxetine[J].Clin Pharmocal Bull(中国药理学通报),2011,27(9):593-597.
[13]	Hu XY, Fei HZ, Jiang XH, et al. Improving oxidative stress response and reversing abnormal expression of hippocampus NET and 5-HTT involved in anti-depressive effect of venlafaxine[J].Clin Pharmocal Bull(中国药理学通报),2014,30(6):848-852.
[14]	Wang LJ,Qiu HM,Jiang XH,et al.Down-regulation of monoamine neurotransmitter synthetase expression involved in CUS-induced depression of rats[J].Chin Pharmacol Bull(中国药理学通报),2015,31(4):527-531.
[15]	Qin LJ,Liu D,Jiang XH,et al.Down-regulation of hyppocampal TH and TPH expression involved in CUMS induced depressive behaviors of rats and interfering effects of venlafaxine[J].Chin J Clin Pharm Ther(中国临床药理学与治疗学),2014,19(4):398-403.

[1]	ZHAO Xiaomei, WANG Xin, JI Xia, Zhang Jing, ZHANG Hua, HU Guoqiang. Synthesis and antitumor activity of heteroatom-substituted azulenes derivatives of 1,2-benzothiazine[J]. Journal of China Pharmaceutical University, 2024, 55(5): 634-638. DOI: 10.11665/j.issn.1000-5048.2023120503
[2]	SUN Dongyu, GONG Jingxu, LI Xuwen, HAN Guanying, GUO Yuewei. Studies on methyl xestopongoate analogues:design, synthesis and antitumor activities[J]. Journal of China Pharmaceutical University, 2018, 49(4): 413-421. DOI: 10.11665/j.issn.1000-5048.20180405
[3]	LI Mingtao, XU Dan, XUE Wenjun, YOU Qidong, SUN Liping. Design, synthesis and antitumor activities of oxazolo［5, 4-d］pyrimidine derivatives[J]. Journal of China Pharmaceutical University, 2017, 48(4): 425-431. DOI: 10.11665/j.issn.1000-5048.20170406
[4]	QIAO Yixue, MOU Yi, HUANG Zhangjian, AI Yong, KANG Fenghua, LAI Yisheng, ZHANG Yihua. Synthesis and antitumor activities of novel CDDO-Me analogues[J]. Journal of China Pharmaceutical University, 2015, 46(3): 289-293. DOI: 10.11665/j.issn.1000-5048.20150305
[5]	MAO Chunfang, XU Chenjun, WANG Xuemin, LI Xianghua, ZHANG Wei, WANG Xinyang, LING Yong. Design, synthesis and antitumor activity of farnesylthiosalicylamide derivatives[J]. Journal of China Pharmaceutical University, 2015, 46(2): 162-168. DOI: 10.11665/j.issn.1000-5048.20150204
[6]	HU Kun, ZHOU Anfei, JIANG Hefei, XU Yuanyuan, HUANG Qianhui, YANG Jie, CHEN Xin, REN Jie. Synthesis and antitumor activities of podophyllotoxin derivatives[J]. Journal of China Pharmaceutical University, 2014, 45(1): 33-38. DOI: 10.11665/j.issn.1000-5048.20140105
[7]	CHENG Keguang, CHU Xiangwu, LIU Guanyan, SU Chunhua, DENG Shengping, PAN Hongmei. Synthesis and antitumor activities of 3-substituted estradiol-pyrimidine derivatives[J]. Journal of China Pharmaceutical University, 2014, 45(1): 26-32. DOI: 10.11665/j.issn.1000-5048.20140104
[8]	LIU Dexiu, PAN Huaying, SHI Hui, HUANG Xiaoying, LOU Hongxiang. Synthesis and antitumor activity of a novel bisbibenzyl derivative[J]. Journal of China Pharmaceutical University, 2013, 44(6): 515-519. DOI: 10.11665/j.issn.1000-5048.20130605
[9]	HU Kun, XU Hua-jin, XIN Wen-qun, CHEN Xin, REN Jie. Synthesis and antitumor activity of nitrogen mustard derivatives of formononetin[J]. Journal of China Pharmaceutical University, 2012, 43(2): 113-119.
[10]	ZHA Xiao-ming, ZHANG Fei-ran, SHAN Jia-qi, CHEN Yan-ke, ZHANG Yi-hua, LIU Jun, SUN Hong-bin. Synthesis and in vitro antitumor activities of novel soladulcidine derivatives[J]. Journal of China Pharmaceutical University, 2010, 41(6): 493-498.

Cited By

Get Citation

PDF

XML

Article views (808) PDF downloads (1684)

Turn off MathJax

Article Contents

Abstract

References

Improvement of NET and 5-HTT expression involved in the anti-depressive effect of valproate

Abstract

References

Related Articles

Catalog

Improvement of NET and 5-HTT expression involved in the anti-depressive effect of valproate

Abstract

References

Related Articles

Catalog

Export File

Citation

Format

Content