Rhein:a novel matrix for profiling and imaging of endogenous metabolites by matrix-assisted laser desorption/ionization-mass spectrometry

YE Hui; WANG Yun; WANG Lin; XU Xiaowei; WU Mengqiu; CAO Guoxiu; LIANG Yan; WANG Guangji

doi:10.11665/j.issn.1000-5048.20160616

Journal of China Pharmaceutical University > 2016 > 47(6): 727-733. > DOI: 10.11665/j.issn.1000-5048.20160616

YE Hui, WANG Yun, WANG Lin, XU Xiaowei, WU Mengqiu, CAO Guoxiu, LIANG Yan, WANG Guangji. Rhein:a novel matrix for profiling and imaging of endogenous metabolites by matrix-assisted laser desorption/ionization-mass spectrometry[J]. Journal of China Pharmaceutical University, 2016, 47(6): 727-733. DOI: 10.11665/j.issn.1000-5048.20160616

Citation:

PDF (2118 KB)

Rhein:a novel matrix for profiling and imaging of endogenous metabolites by matrix-assisted laser desorption/ionization-mass spectrometry

More Information

Graphical Abstract

Abstract

Abstract

MALDI-MS is an emerging technique that enables rapid profiling of endogenous substances in a high throughput. Nevertheless, its application to metabolite analysis is often hindered by the presence of massive matrix background peaks using conventional matrices. Herein rhein was introduced as a novel matrix for MALDI-MS analysis of cation metabolites. Several strong and weak basic metabolites were measured using rhein. Compared to the previously reported ionless matrix NSA and conventional matrix CHCA, clean MALDI-MS spectra were obtained devoid of matrix signals. Subsequently, we applied this matrix to profile a biologically complex sample, mice intestinal contents. In addition, we have validated the applicability of rhein to MALDI-MS imaging, which allows for simultaneous mapping of hundreds of metabolites from single mouse ileum section. In summary, the discovery of rhein as a novel MALDI matrix enables efficient and reliable profiling and imaging of endogenous metabolites from biological samples with no matrix interferences, which will greatly promote the utility of MALDI-MS-based platform for metabolome studies.
- MALDI-MS,
- mass spectrometry imaging,
- novel matrix,
- rhein,
- metabonomics

FullText(HTML)

References (22)

References

[1]	Holmes E,Wilson ID,Nicholson JK.Metabolic phenotyping in health and disease[J].Cell,2008,134(5):714-717.
[2]	Nicholson JK,Holmes E,Kinross JM,et al.Metabolic phenotyping in clinical and surgical environments[J].Nature,2012,491(7424):384-392.
[3]	Janeková H,Hron K,Wojtowicz P,et al.Targeted metabolomic analysis of plasma samples for the diagnosis of inherited metabolic disorders[J].J Chromatogr A,2012,1226(4):11-17.
[4]	Girlanda R,Cheema AK,Kaur P,et al.Metabolomics of human intestinal transplant rejection[J].Am J Transplant,2012(12):S18-S26.
[5]	Zhang AH,Sun H,Yan GL,et al.Metabolomics study of type 2 diabetes using ultra-performance LC-ESI/quadrupole-TOF high-definition MS coupled with pattern recognition methods[J].J Physiol Biochem,2014,70(1):117-128.
[6]	Duscharla D,Bhumireddy SR,Lakshetti S,et al.Prostate cancer associated lipid signatures in serum studied by ESI-tandem mass spectrometry as potential new biomarkers[J].PLoS One,2016,11(3):e0150253.
[7]	Liu Y,Hong ZY,Tan G,et al.NMR and LC/MS-based global metabolomics to identify serum biomarkers differentiating hepatocellular carcinoma from liver cirrhosis[J].Int J Cancer,2014,135(3):658-668.
[8]	Buck A,Walch A.In situ drug and metabolite analysis in biological and clinical research by MALDI MS imaging[J].Bioanalysis,2014,6(9):1241-1253.
[9]	Ye H,Wang JX,Greer T,et al.Visualizing neurotransmitters and netabolites in the central nervous system by high resolution and high accuracy mass spectrometric imaging[J].ACS Chem Neurosci,2013,4(7):1049-1056.
[10]	Wang J,Qiu S,Chen S,et al.MALDI-TOF MS imaging of metabolites with a N-(1-naphthyl)ethylenediamine dihydrochloride matrix and its application to colorectal cancer liver metastasis[J].Anal Chem,2015,87(1):422-430.
[11]	Römpp A,Spengler B.Mass spectrometry imaging with high resolution in mass and space[J].Histochem Cell Biol,2013,139(6):759-783.
[12]	Morosi L,Spinelli P,Zucchetti M,et al.Determination of paclitaxel distribution in solid tumors by nano-particle assisted laser desorption ionization mass spectrometry imaging[J].PLoS One,2013,8(8):e72532.
[13]	Shroff R,Svatos A.Proton sponge:a novel and versatile MALDI matrix for the analysis of metabolites using mass spectrometry[J].Anal Chem,2009,81(19):7954-7959.
[14]	Strupat K,Karas M,Hillenkamp F.2,5-Dihydroxybenzoic acid:a new matrix for laser desorption-ionization mass spectrometry[J].Int J Mass Spectrom,1991,111:89-102.
[15]	Shariatgorji M,Källback P,Gustavsson L,et al.Controlled-pH tissue cleanup protocol for signal enhancement of small molecule drugs analyzed by MALDI-MS imaging[J].Anal Chem,2012,84(10):4603-4607.
[16]	Fukuyama Y, Nakajima C, Furuichi K, et al. Alkylated trihydroxyacetophenone as a MALDI matrix for hydrophobic peptides[J].Anal Chem,2013,85(20):9444-9448.
[17]	Shroff R, Rulíek L, Doubsk J, et al. Acid-base-driven matrix-assisted mass spectrometry for targeted metabolomics[J].Proc Natl Acad Sci U S A,2009,106(25):10092-10096.
[18]	Wang Z,Klipfell E,Bennett BJ,et al.Gut flora metabolism of phosphatidylcholine promotes cardiovascular disease[J].Nature,2011,472(7341):57-63.
[19]	Tang WH,Hazen SL.The contributory role of gut microbiota in cardiovascular disease[J].J Clin Invest,2014,124(10):4204-4211.
[20]	Zhu W,Gregory JC,Org E,et al.Gut microbial metabolite TMAO enhances platelet hyperreactivity and thrombosis risk[J].Cell,2016,165(1):111-124.
[21]	Tian Y,Zhang L,Wang Y,et al.Age-related topographical metabolic signatures for the rat gastrointestinal contents[J].J Proteome Res,2012,11(2):1397-1411.
[22]	Bergen WG,Wu G.Intestinal nitrogen recycling and utilization in health and disease[J].J Nutr,2009,139(5):821-825.

[1]	QIAN Xiuhui, SUN Jing, FU San, TANG Xiaoyan, XU Xianghong, ZHANG Mian. Effect of intratracheal instillation of PM_2.5suspensionon pulmonary fibrosis in mice and the intervention of neotuberostemonine[J]. Journal of China Pharmaceutical University, 2021, 52(4): 455-462. DOI: 10.11665/j.issn.1000-5048.20210408
[2]	FANG Daqing, LIU Fang, ZHANG Sijia, CAI Chaoqun, LIU Minzhuo, LIU Jinghan, YANG Chunhua, WANG Hao. One new diterpenoid alkaloid from Aconitum carmichaeli[J]. Journal of China Pharmaceutical University, 2017, 48(5): 568-571. DOI: 10.11665/j.issn.1000-5048.20170510
[3]	XIANG Juan, YU Ping, LI Mingdan, ZHANG Chaofeng, XU Xianghong, ZHANG Mian. Protective effects of stemona alkaloids on mice with bleomycin-induced pulmonary fibrosis[J]. Journal of China Pharmaceutical University, 2017, 48(1): 76-81. DOI: 10.11665/j.issn.1000-5048.20170112
[4]	YAO Juan, XIONG Fei, ZHU Zi-yi, LIU Jian-ping, GU Ning. Preparation and in vitro evaluation of vincristine-loaded superparamagnetic iron oxide nanoparticles[J]. Journal of China Pharmaceutical University, 2012, 43(3): 222-225.
[5]	Comparative pharmacokinetics of aconitine, mesaconitine and hypaconitine in rats after oral administration of four decoctions composed with Radix Aconiti Lateralis[J]. Journal of China Pharmaceutical University, 2010, 41(1): 55-59.
[10]	POLLEN MORPHOLOGY IN CHINESE SPECIES OF STEMONA[J]. Journal of China Pharmaceutical University, 1989, (6): 325-329.

Cited By

Get Citation

PDF

XML

Article views (925) PDF downloads (1454)

Turn off MathJax

Article Contents

Abstract

References

Rhein:a novel matrix for profiling and imaging of endogenous metabolites by matrix-assisted laser desorption/ionization-mass spectrometry

Abstract

References

Related Articles

Catalog

Rhein:a novel matrix for profiling and imaging of endogenous metabolites by matrix-assisted laser desorption/ionization-mass spectrometry

Abstract

References

Related Articles

Catalog

Export File

Citation

Format

Content