• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
LIU Li, PEI Siran, WU Huali, ZHAO Qingshun, PENG Jixian, SHANG Jing. Drug screening model of treating pigmentation disorders in tyrp1a transgenic zebrafish[J]. Journal of China Pharmaceutical University, 2016, 47(6): 740-743. DOI: 10.11665/j.issn.1000-5048.20160618
Citation: LIU Li, PEI Siran, WU Huali, ZHAO Qingshun, PENG Jixian, SHANG Jing. Drug screening model of treating pigmentation disorders in tyrp1a transgenic zebrafish[J]. Journal of China Pharmaceutical University, 2016, 47(6): 740-743. DOI: 10.11665/j.issn.1000-5048.20160618
shu

Drug screening model of treating pigmentation disorders in tyrp1a transgenic zebrafish

More Information
    Graphical Abstract
    • Abstract
  • To effectively screen treating pigmentation disorders drug, a new transgenic zebrafish drug screening model was constructed. Green fluorescent protein(GFP)were drived by tyrosinase-related protein 1a (tyrp1a)promoter specific expression in melanocyte. Effect of N-Phenylthiourea(PTU)and alpha-melanaocyte stimulating hormone(α-MSH)on the GFP expression and melanogenic of tyrp1a: eGFP zebrafish were photographed under the steromicroscope. Results showed that PTU could significantly inhibit the melanogenic and expression of GFP of zebrafish. As compared with control group, α-MSH treatment resulted in marked stimulation of body pigmentation and expression of GFP. In conclusion, a new transgenic zebrafish drug screening model was successfully established, which can be used for treating pigmentation disorders.
    • FullText(HTML)
    • References (13)
  • [1]
    Nicolaidou E,Katsambas AD.Pigmentation disorders:hyperpigmentation and hypopigmentation[J].Clin Dermatol,2014,32(1):66-72.
    [2]
    Zhou J,Song J,Ping F,et al.Enhancement of the p38 MAPK and PKA signaling pathways is associated with the pro-melanogenic activity of interleukin 33 in primary melanocytes[J].J Dermatol Sci,2014,73(2):110-116.
    [3]
    Wu HL,Pang SL,Liu QZ,et al.5-HT1A/1B receptors as targets for optimizing pigmentary responses in C57BL/6 mouse skin to stress[J].PLoS One,2014,9(2):e89663.
    [4]
    Pang S, Wu H, Wang Q, et al. Chronic stress suppresses the expression of cutaneous hypothalamic-pituitary-adrenocortical axis elements and melanogenesis[J].PLoS One,2014,9(5):e98283.
    [5]
    Zhou J, Shang J, Ping F, et al. Alcohol extract from Vernonia anthelmintica(L.)wild seed enhances melanin synthesis through activation of the p38 MAPK signaling pathway in B16F10 cells and primary melanocytes[J].J Ethnopharmacol,2012,143(2):639-647.
    [6]
    Wang TJ,An J,Chen XH,et al.Assessment of Cuscuta chinensis seeds effect on melanogenesis:comparison of water and ethanol fractions in vitro and in vivo[J].J Ethnopharmacol,2014,154(1):240-248.
    [7]
    Wang CM,Jing LJ,Wei YJ,et al.Evaluation of antiosteoporotic activity for mico-amount asperosaponins V and VI by on the osteoporosis model using zebrafish[J].J China Pharm Univ(中国药科大学学报),2014,45(1):88-91.
    [8]
    Choi TY,Kim JH,Ko DH,et al.Zebrafish as a new model for phenotype-based screening of melanogenic regulatory compounds[J].Pigment Cell Res,2007,20(2):120-127.
    [9]
    Böhm M,Luger TA.Alpha-melanocyte-stimulating hormone.From bench to bedside[J].Hautarzt,2010,61(6):497-504.
    [10]
    Zon LI,Peterson RT.In vivo drug discovery in the zebrafish[J].Nat Rev Drug Discov,2005,4(1):35-44.
    [11]
    Boverhof DR,Chamberlain MP,Elcombe CR,et al.Transgenic animal models in toxicology:historical perspectives and future outlook[J].Toxicol Sci,2011,121(2):207-233.
    [12]
    Westerfield M.The zebrafish book:a guide for the laboratory use of zebrafish(Danio rerio)[M].University of Oregon Press,2000:55.
    [13]
    Jin EJ,Thibaudeau G.Effects of lithium on pigmentation in the embryonic zebrafish[J].Biochim Biophys Acta,1999,1449(1):93-99.
    • Related Articles

  • Related Articles

    [1]MO Xinhe, WAN Youqiong, WANG Sibu, MA Qin, ZHANG Jun, CHEN Ying. Ameliorative effect of baicalin nanomedicine on hydrogen peroxide-induced senescence of human umbilical vein vascular endothelial cells[J]. Journal of China Pharmaceutical University, 2025, 56(1): 110-118. DOI: 10.11665/j.issn.1000-5048.2024052101
    [2]WANG Dandan, KORAMAGAZI Arouna, MAO Yong, YU Feng. Rhein induces apoptosis in L-02 cells via reactive oxygen species-independent endoplasmic reticulum stress pathway[J]. Journal of China Pharmaceutical University, 2016, 47(2): 215-221. DOI: 10.11665/j.issn.1000-5048.20160215
    [3]SHEN Kai, KOU Junping, YU Boyang. Research advances in the mechanisms of active components in Chinese materia medica against oxidative stress-induced neuronal apoptosis[J]. Journal of China Pharmaceutical University, 2015, 46(5): 532-540. DOI: 10.11665/j.issn.1000-5048.20150503
    [4]WANG Li, LI Mingdan, LU Yi, ZHANG Chaofeng, XU Xianghong, ZHANG Mian. Mechanism of apoptosis induced by peptide-rich fraction from the root of Aster tataricus in liver L-02 cells[J]. Journal of China Pharmaceutical University, 2014, 45(4): 469-474. DOI: 10.11665/j.issn.1000-5048.20140415
    [5]QI Cuiling, ZHOU Xinlei, YE Jie, YANG Yang, ZHANG Qianqian, LI Jiangchao, WANG Lijing. Andrographolide induces Tb cell apoptosis by activating Caspase-3/PARP[J]. Journal of China Pharmaceutical University, 2013, 44(6): 559-562. DOI: 10.11665/j.issn.1000-5048.20130614
    [6]LIU Zhi-yong, NIU Zhi-yuan, ZHENG Wei, SHEN Ping-ping. Effects of p-ERK1/2 on nitric oxide donor induced apoptosis of HepG2 cells[J]. Journal of China Pharmaceutical University, 2012, 43(6): 530-534.
    [7]Inhibition of Hydrogen Peroxide-induced PC12 Cell Apoptosis by Modafinil[J]. Journal of China Pharmaceutical University, 2004, (3): 77-81.
    [8]Protective Effects of MCl-186 on Apoptosis in PC12 Cells Induced by H2O2[J]. Journal of China Pharmaceutical University, 2004, (1): 62-66.
    [9]Effects of Crocetin on the Myocardial Cell Damages Due to Oxidative Stress[J]. Journal of China Pharmaceutical University, 2003, (5): 66-69.
    [10]Inhibition of Puerarin on the H_2O_2-induced Apoptosis of Smooth Muscle Cells[J]. Journal of China Pharmaceutical University, 2002, (3): 75-78.

Catalog

    Get Citation
    PDF
    XML
    Article views (955) PDF downloads (2712) Cited by()
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles

    Copyright © Journal of China Pharmaceutical University苏ICP备12050101号-2

    Address：24 Tongjia Lane, Nanjing City, Jiangsu Province （210009）Tel: 025-83271566E-mail: xuebao@cpu.edu.cn

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    Total visits：300495

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return