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LIU Li, PEI Siran, WU Huali, ZHAO Qingshun, PENG Jixian, SHANG Jing. Drug screening model of treating pigmentation disorders in tyrp1a transgenic zebrafish[J]. Journal of China Pharmaceutical University, 2016, 47(6): 740-743. DOI: 10.11665/j.issn.1000-5048.20160618
Citation: LIU Li, PEI Siran, WU Huali, ZHAO Qingshun, PENG Jixian, SHANG Jing. Drug screening model of treating pigmentation disorders in tyrp1a transgenic zebrafish[J]. Journal of China Pharmaceutical University, 2016, 47(6): 740-743. DOI: 10.11665/j.issn.1000-5048.20160618

Drug screening model of treating pigmentation disorders in tyrp1a transgenic zebrafish

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  • To effectively screen treating pigmentation disorders drug, a new transgenic zebrafish drug screening model was constructed. Green fluorescent protein(GFP)were drived by tyrosinase-related protein 1a (tyrp1a)promoter specific expression in melanocyte. Effect of N-Phenylthiourea(PTU)and alpha-melanaocyte stimulating hormone(α-MSH)on the GFP expression and melanogenic of tyrp1a: eGFP zebrafish were photographed under the steromicroscope. Results showed that PTU could significantly inhibit the melanogenic and expression of GFP of zebrafish. As compared with control group, α-MSH treatment resulted in marked stimulation of body pigmentation and expression of GFP. In conclusion, a new transgenic zebrafish drug screening model was successfully established, which can be used for treating pigmentation disorders.
  • [1]
    Nicolaidou E,Katsambas AD.Pigmentation disorders:hyperpigmentation and hypopigmentation[J].Clin Dermatol,2014,32(1):66-72.
    [2]
    Zhou J,Song J,Ping F,et al.Enhancement of the p38 MAPK and PKA signaling pathways is associated with the pro-melanogenic activity of interleukin 33 in primary melanocytes[J].J Dermatol Sci,2014,73(2):110-116.
    [3]
    Wu HL,Pang SL,Liu QZ,et al.5-HT1A/1B receptors as targets for optimizing pigmentary responses in C57BL/6 mouse skin to stress[J].PLoS One,2014,9(2):e89663.
    [4]
    Pang S, Wu H, Wang Q, et al. Chronic stress suppresses the expression of cutaneous hypothalamic-pituitary-adrenocortical axis elements and melanogenesis[J].PLoS One,2014,9(5):e98283.
    [5]
    Zhou J, Shang J, Ping F, et al. Alcohol extract from Vernonia anthelmintica(L.)wild seed enhances melanin synthesis through activation of the p38 MAPK signaling pathway in B16F10 cells and primary melanocytes[J].J Ethnopharmacol,2012,143(2):639-647.
    [6]
    Wang TJ,An J,Chen XH,et al.Assessment of Cuscuta chinensis seeds effect on melanogenesis:comparison of water and ethanol fractions in vitro and in vivo[J].J Ethnopharmacol,2014,154(1):240-248.
    [7]
    Wang CM,Jing LJ,Wei YJ,et al.Evaluation of antiosteoporotic activity for mico-amount asperosaponins V and VI by on the osteoporosis model using zebrafish[J].J China Pharm Univ(中国药科大学学报),2014,45(1):88-91.
    [8]
    Choi TY,Kim JH,Ko DH,et al.Zebrafish as a new model for phenotype-based screening of melanogenic regulatory compounds[J].Pigment Cell Res,2007,20(2):120-127.
    [9]
    Böhm M,Luger TA.Alpha-melanocyte-stimulating hormone.From bench to bedside[J].Hautarzt,2010,61(6):497-504.
    [10]
    Zon LI,Peterson RT.In vivo drug discovery in the zebrafish[J].Nat Rev Drug Discov,2005,4(1):35-44.
    [11]
    Boverhof DR,Chamberlain MP,Elcombe CR,et al.Transgenic animal models in toxicology:historical perspectives and future outlook[J].Toxicol Sci,2011,121(2):207-233.
    [12]
    Westerfield M.The zebrafish book:a guide for the laboratory use of zebrafish(Danio rerio)[M].University of Oregon Press,2000:55.
    [13]
    Jin EJ,Thibaudeau G.Effects of lithium on pigmentation in the embryonic zebrafish[J].Biochim Biophys Acta,1999,1449(1):93-99.
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