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LIU Li, PEI Siran, WU Huali, ZHAO Qingshun, PENG Jixian, SHANG Jing. Drug screening model of treating pigmentation disorders in tyrp1a transgenic zebrafish[J]. Journal of China Pharmaceutical University, 2016, 47(6): 740-743. DOI: 10.11665/j.issn.1000-5048.20160618
Citation: LIU Li, PEI Siran, WU Huali, ZHAO Qingshun, PENG Jixian, SHANG Jing. Drug screening model of treating pigmentation disorders in tyrp1a transgenic zebrafish[J]. Journal of China Pharmaceutical University, 2016, 47(6): 740-743. DOI: 10.11665/j.issn.1000-5048.20160618
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Drug screening model of treating pigmentation disorders in tyrp1a transgenic zebrafish

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    • Abstract
  • To effectively screen treating pigmentation disorders drug, a new transgenic zebrafish drug screening model was constructed. Green fluorescent protein(GFP)were drived by tyrosinase-related protein 1a (tyrp1a)promoter specific expression in melanocyte. Effect of N-Phenylthiourea(PTU)and alpha-melanaocyte stimulating hormone(α-MSH)on the GFP expression and melanogenic of tyrp1a: eGFP zebrafish were photographed under the steromicroscope. Results showed that PTU could significantly inhibit the melanogenic and expression of GFP of zebrafish. As compared with control group, α-MSH treatment resulted in marked stimulation of body pigmentation and expression of GFP. In conclusion, a new transgenic zebrafish drug screening model was successfully established, which can be used for treating pigmentation disorders.
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    • References (13)
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