• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
XIANG Juan, YU Ping, LI Mingdan, ZHANG Chaofeng, XU Xianghong, ZHANG Mian. Protective effects of stemona alkaloids on mice with bleomycin-induced pulmonary fibrosis[J]. Journal of China Pharmaceutical University, 2017, 48(1): 76-81. DOI: 10.11665/j.issn.1000-5048.20170112
Citation: XIANG Juan, YU Ping, LI Mingdan, ZHANG Chaofeng, XU Xianghong, ZHANG Mian. Protective effects of stemona alkaloids on mice with bleomycin-induced pulmonary fibrosis[J]. Journal of China Pharmaceutical University, 2017, 48(1): 76-81. DOI: 10.11665/j.issn.1000-5048.20170112
shu

Protective effects of stemona alkaloids on mice with bleomycin-induced pulmonary fibrosis

More Information
    Graphical Abstract
    • Abstract
  • This study aimed to investigate the protective effect of total alkaloids(TA)and one of the active components, neotuberostemonine, of Stemona tuberosa on bleomycin-induced pulmonary fibrosis in mice and to explore the mechanism by fibroblasts model. The bleomycin-induced mice were orally administered with TA(60 mg/kg)and neotuberostemonine(10, 20 mg/kg), with prednisone(6. 67 mg/kg)as a positive control. The anti-fibrotic effects were assessed by hydroxyproline content, TGF-β1 level, inflammatory score, collagen deposition and the expression of α-SMA in the lung tissues. The results revealed that TA and neotuberostemonine could significantly ameliorate the inflammation and injury, and attenuate the hydroxyproline content and collagen deposition. Moreover, neotuberostemonine treatment markedly down-regulated the α-SMA level and TGF-β1 content in bleomycin-injured mice lungs. The in vitro experiments showed that neotuberostemonine inhibited the expression of α-SMA induced by TGF-β1 in a dose-dependent manner, indicating that suppression on differentiation of fibroblasts to myofibroblasts may be one of the mechanisms for neotuberostemonine against pulmonary fibrosis.
    • FullText(HTML)
    • References (16)
  • [1]
    Wolters PJ, Collard HR, Jones KD. Pathogenesis of idiopathic pulmonary fibrosis[J].Annu Rev Pathol,2014,9:157-179.
    [2]
    Karimi-Shah BA,Chowdhury BA.Forced vital capacity in idiopathic pulmonary fibrosis—FDA review of pirfenidone and nintedanib[J].N Engl J Med,2015,372(13):1189-1191.
    [3]
    Lekkerkerker AN,Aarbiou J,van Es T,et al.Cellular players in lung fibrosis[J].Curr Pharm Des,2012,18(27):4093-4102.
    [4]
    Chinese Pharmacopoeia Commission.Chinese Pharmacopoeia:part 1(中华人民共和国药典:一部)[S].Beijing:China Medical Science Press,2015:132.
    [5]
    Gao Y,Wang J,Zhang CF,et al.Seven new alkaloids from the roots of Stemona tuberosa[J]. Tetrahedron,2014,70(4):967-974.
    [6]
    Chung HS,Hon PM,Lin G,et al.Antitussive activity of Stemona alkaloids from Stemona tuberosa[J].Planta Med,2003,69(10):914-920.
    [7]
    Sun H,Dong W,Zhang A,et al.Ultra-performance liquid-chromatography with tandem mass spectrometry performing pharmacokinetic and biodistribution studies of croomine,neotuberostemonine and tuberostemonine alkaloids absorbed in the rat plasma after oral administration of Stemonae Radix[J].Fitoterapia,2012,83(8):1699-1705.
    [8]
    Lv XX,Wang XX,Li K,et al.Rupatadine protects against pulmonary fibrosis by attenuating PAF-mediated senescence in rodents[J]. PLoS ONE,2013,8(7):e68631.
    [9]
    You XY,Xue Q,Fang Y,et al.Preventive effects of Ecliptae Herba extract and its component,ecliptasaponin A,on bleomycin-induced pulmonary fibrosis in mice[J].J Ethnopharmacol,2015,175:172-180.
    [10]
    Seluanov A,Vaidya A,Gorbunova V.Establishing primary adult fibroblast cultures from rodents[J].J Vis Exp,2010(44):2033.
    [11]
    Jung KH,Choi HL,Park S,et al.The effects of the standardized herbal formula PM014 on pulmonary inflammation and airway responsiveness in a murine model of cockroach allergen-induced asthma[J].J Ethnopharmacol,2014,155(1):113-122.
    [12]
    Wei JS,Li JY,Mei RH,et al.Influence of the extract of Compound Formula Rosin and Bletilla Table in bleomycin induced pulmonary fibrosis in mice[J].China Med Her(中国医药导报),2012,9(28):24-26.
    [13]
    Lim D,Lee E,Jeong E,et al.Stemona tuberosa prevented inflammation by suppressing the recruitment and the activation of macrophages in vivo and in vitro[J].J Ethnopharmacol,2015,160:41-51.
    [14]
    Raghu G,Collard HR,Egan JJ,et al.An official ATS/ERS/JRS/ALAT statement:idiopathic pulmonary fibrosis:evidence-based guidelines for diagnosis and management[J].Am J Respir Crit Care Med,2011,183(6):788-824.
    [15]
    Roy SG,Nozaki Y,Phan SH.Regulation of alpha-smooth muscle actin gene expression in myofibroblast differentiation from rat lung fibroblasts[J].Int J Biochem Cell Biol,2001,33(7):723-734.
    [16]
    Della Latta V,Cecchettini A,Del Ry S,et al.Bleomycin in the setting of lung fibrosis induction:from biological mechanisms to counteractions[J].Pharmacol Res,2015,97:122-130.
    • Related Articles

  • Related Articles

    [1]CHEN Yanqing, GONG Ping, LIU Zhen, ZHANG Yan, YU Yang. Serum amyloid A 3 deficiency improves cognitive impairment and attenuates tau pathology in mouse model of Alzheimer′s disease[J]. Journal of China Pharmaceutical University, 2021, 52(5): 586-595. DOI: 10.11665/j.issn.1000-5048.20210511
    [2]CHEN Yingjie, GAO Xiangdong, CHEN Song. Effects and mechanisms of FGF21 on neuronal damage induced by rotenone[J]. Journal of China Pharmaceutical University, 2020, 51(6): 718-723. DOI: 10.11665/j.issn.1000-5048.20200611
    [3]SUN Yan, GAO Xiangdong, CHEN Song. Effect and mechanism of FGF21 on astrocyte damage induced by Aβ25-35[J]. Journal of China Pharmaceutical University, 2019, 50(4): 490-496. DOI: 10.11665/j.issn.1000-5048.20190415
    [4]CHEN Suting, CHEN Song, GAO Xiangdong. Construction, expression, purification and neuroprotective activity of TAT-FGF21 fusion protein[J]. Journal of China Pharmaceutical University, 2018, 49(4): 496-501. DOI: 10.11665/j.issn.1000-5048.20180417
    [5]YU Haiyang, CHEN Song, XU Zheng, GAO Xiangdong, YAO Wenbing. Protective effect of fibroin peptides on Aβ25-35-induced injury in SH-SY5Y cells and its mechanism[J]. Journal of China Pharmaceutical University, 2017, 48(5): 609-613. DOI: 10.11665/j.issn.1000-5048.20170517
    [6]WANG Dandan, KORAMAGAZI Arouna, MAO Yong, YU Feng. Rhein induces apoptosis in L-02 cells via reactive oxygen species-independent endoplasmic reticulum stress pathway[J]. Journal of China Pharmaceutical University, 2016, 47(2): 215-221. DOI: 10.11665/j.issn.1000-5048.20160215
    [7]CHEN Jianping, REN Xinsheng, SUN Zhonghua, GUO Zaiyu. Protective effects and mechanisms of breviscapine on endothelial cells[J]. Journal of China Pharmaceutical University, 2015, 46(5): 610-616. DOI: 10.11665/j.issn.1000-5048.20150516
    [8]Effects of MCI-186 on Oxidative Stress-mediated Cell Damage Induced by H2O2 and Reactive Oxygen Species Formation in Brain Neurons[J]. Journal of China Pharmaceutical University, 2004, (6): 84-87.
    [9]WU Yu-Lin, YANG Zheng. Effects of MCI-186 on Oxidative Stress-mediated Cell Damage Induced by H_2O_2 and Reactive Oxygen Species Formation in Brain Neurons[J]. Journal of China Pharmaceutical University, 2004, (6).
    [10]Effects of Crocetin on the Myocardial Cell Damages Due to Oxidative Stress[J]. Journal of China Pharmaceutical University, 2003, (5): 66-69.

  • Cited by

    Periodical cited type(1)

    1. 周霞辉，罗晓欣，周曼丽，俞赟丰，赵彦禛，张宜帆，简维雄. 冠状动脉粥样硬化性心脏病血瘀证表观遗传学研究进展. 中国中医药信息杂志. 2022(06): 148-152 .

    Other cited types(1)

Catalog

    Get Citation
    PDF
    XML
    Article views (853) PDF downloads (2026) Cited by(2)
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles

    Copyright © Journal of China Pharmaceutical University苏ICP备12050101号-2

    Address：24 Tongjia Lane, Nanjing City, Jiangsu Province （210009）Tel: 025-83271566E-mail: xuebao@cpu.edu.cn

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    Total visits：304019

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return