• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
YAO Jing, SUN Jianguo, WU Liang, WANG Hao, WANG Guangji, YE Wencai. Identification of major constituents from propolis by LC-IT-TOF-MS[J]. Journal of China Pharmaceutical University, 2017, 48(2): 178-183. DOI: 10.11665/j.issn.1000-5048.20170208
Citation: YAO Jing, SUN Jianguo, WU Liang, WANG Hao, WANG Guangji, YE Wencai. Identification of major constituents from propolis by LC-IT-TOF-MS[J]. Journal of China Pharmaceutical University, 2017, 48(2): 178-183. DOI: 10.11665/j.issn.1000-5048.20170208

Identification of major constituents from propolis by LC-IT-TOF-MS

More Information
  • A qualitative analytical method of liquid chromatography coupled with ion trap time-of-flight mass spectrometry(LC-IT-TOF/MS)was developed for identification of major constituents in propolis from China(Shandong). The LC-IT-TOF-MS was performed on a Shim-pack VP-ODS column(2. 0 mm×150 mm, 5 μm)with the mobile phase consisting of acetonitrile and water containing 0. 2% formic acid in gradient mode, and the flow rate was set at 0. 3 mL/min. Negative ion mode was used for IT-TOF-MS. According to the accurate molecular weight, MS fragment pathway, comparison with the retention time of reference compounds, total 31 compounds, including twenty-five phenolic acids and six flavonoids were identified. The LC-IT-TOF-MS qualitative analysis method can be used for analyzing major components of propolis quickly and accurately.
  • [1]
    Gardana C,Scaglianti M,Pietta P,et al.Analysis of the polyphenolic fraction of propolis from different sources by liquid chromatography-tandem mass spectrometry[J].J Pharm Biomed Anal,2007,45(3):390-399.
    [2]
    Kumazawa S,Hamasaka T,Nakayama T.Antioxidant activity of propolis of various geographic origins[J].Food Chem,2004,84(3):329-339.
    [3]
    Wang K,Zhang CP,Hu F.Research status of propolis in 2014[J].J Bee(蜜蜂杂志),2015,35(3):1-6.
    [4]
    Zhang YZ,Xu F,Zhang JY,et al.Investigations of the fragmentation behavior of 11 isoflavones with ESI-IT-TOF-MSn[J].J China Pharm Sci(中国药学),2014,23(9):631-641.
    [5]
    Liang Y,Hao HP,et al.Qualitative and quantitative determination of complicated herbal components by liquid chromatography hybrid ion trap time-of-flight mass spectrometry and a relative exposure approach to herbal pharmacokinetics independent of standards[J].J Chromatogr A,2010,1217(30):4971-4979.
    [6]
    Zheng CN,Hao HP,Wang X,et al.Diagnostic fragment-ion-based extension strategy for rapid screening and identification of serial components of homologous families contained in traditional Chinese medicine prescription using high-resolution LC-ESI-IT-TOF/MS: Shengmai injectionas an example[J].J Mass Spectrom,2009,44(2):230-244.
    [7]
    Geng CA,Chen XL,Zhou NJ,et al.LC-MS Guided isolation of(± )-Swe-riledugenin A,a pair of enantiomeric lactones,from Swertia leducii[J].Org Lett,2014,16(26):370-373.
    [8]
    Pellati F,Orlandini G,Pinetti D,et al.HPLC-DAD and HPLC-ESI-MS/MS methods for metabolite profiling of propolis extracts[J].J Pharm Biomed Anal,2011,55(5):934-948.
    [9]
    Falcão SI,Vale N,Gomes P,et al.Phenolic profiling of Portuguese propolis by LC-MS spectrometry:uncommon propolis rich in flavonoid glycosides[J].Phytochem Anal,2013,24(4):309-318.
    [10]
    Ding JH,Wang XX,Zhang H,et al.Extrative electrospray ionization tandem mass spectrometry of apigenin[J].Chem J Chin Univ(高等学校化学学报),2011,32(8):1714-1719.
    [11]
    Liu RX,Ye M,Guo HZ,et al.Liquid chromatography/electrospray ionization mass spectrometry for the characterization of twenty-three flavonoids in the extract of Dalbergia odorifera[J].Rapid Commun Mass Spectrom,2005,19(11):1557-1565.
    [12]
    Righi AA,Negri G,Salatino A.Comparative chemistry of propolis from eight Brazilian Localities[J].Evid Based Complement Alternat Med,2013,2013(878):267878.
    [13]
    Wang YX,Hao HP,Wang GJ.Application of Q-TOF and IT-TOF mass spectrometry technology in identifying the natural products and their biological metabolites[J].Chin J Nat Med(中国天然药物),2009,7(5):394-400.
  • Related Articles

    [1]LIANG Jinlai, XU Wei, YIN Tingjie, HUO Meirong. Advances in the hypoxia-responsive antitumor drug nanocarriers and tumor hypoxia relieve[J]. Journal of China Pharmaceutical University, 2018, 49(3): 255-262. DOI: 10.11665/j.issn.1000-5048.20180301
    [2]GUO Yahui, LU Peng, WANG Yubin, ZHANG Huibin. Progress in the researches for antitumor NEDD8 activating enzyme inhibitors[J]. Journal of China Pharmaceutical University, 2017, 48(6): 646-653. DOI: 10.11665/j.issn.1000-5048.20170603
    [3]GAO Yahan, PING Qineng, ZONG Li. Preparation and antitumor activity of mitoxantrone conjugated D-α-tocopherylpolyethylene glycol 1000 succinate prodrug micelle[J]. Journal of China Pharmaceutical University, 2016, 47(3): 311-316. DOI: 10.11665/j.issn.1000-5048.20160311
    [4]LIU Yan, WANG Tingting, CHEN Li. Synthesis and antitumor activity of isosteviol derivatives[J]. Journal of China Pharmaceutical University, 2016, 47(1): 48-53. DOI: 10.11665/j.issn.1000-5048.20160106
    [5]LIU Wenhu, CHANG Jinxia, ZHANG Jianwu. Synthesis and antitumor activities of novel coumarin derivatives containing chalcone moiety[J]. Journal of China Pharmaceutical University, 2014, 45(5): 522-528. DOI: 10.11665/j.issn.1000-5048.20140503
    [6]CHENG Haibo, XU Bin, ZHANG Huibin, ZHOU Jinpei. Synthetic process of antitumor drug dacomitinib[J]. Journal of China Pharmaceutical University, 2014, 45(2): 165-169. DOI: 10.11665/j.issn.1000-5048.20140206
    [7]LI Linhu, CHEN Li, XIA Yufeng. Progress in the study of coumarin derivatives as antitumor agents[J]. Journal of China Pharmaceutical University, 2013, 44(4): 374-379. DOI: 10.11665/j.issn.1000-5048.20130417
    [8]JIN Hui, LIU Lifeng, DENG Weiping, LIU Jianwen. Antitumor effects and molecular mechanisms of novel selective HDAC inhibitors in human cervix carcinoma HeLa cells[J]. Journal of China Pharmaceutical University, 2013, 44(4): 352-356. DOI: 10.11665/j.issn.1000-5048.20130413
    [9]Cooperative Effects of Monkshood Polysaccharide with Adriamycin Magnetic Albumin Microsphere Targeting Therapy on Tumor[J]. Journal of China Pharmaceutical University, 2003, (6): 68-72.
    [10]Studies on the Difference in Content of an Antitumor Component 5F in Pteris semipinnata L.[J]. Journal of China Pharmaceutical University, 2002, (5): 48-50.
  • Cited by

    Periodical cited type(2)

    1. 李焱琳,王琪. 干扰素介导肿瘤免疫分子机制的相关研究进展. 临床合理用药. 2025(02): 177-180 .
    2. 梅家豪,洪泽,王琛. 靶向cGAS-STING信号通路药物的研究进展. 中国药科大学学报. 2020(03): 249-259 . 本站查看

    Other cited types(0)

Catalog

    Article views (932) PDF downloads (1756) Cited by(2)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return