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YU Haiyang, CHEN Song, XU Zheng, GAO Xiangdong, YAO Wenbing. Protective effect of fibroin peptides on Aβ25-35-induced injury in SH-SY5Y cells and its mechanism[J]. Journal of China Pharmaceutical University, 2017, 48(5): 609-613. DOI: 10.11665/j.issn.1000-5048.20170517
Citation: YU Haiyang, CHEN Song, XU Zheng, GAO Xiangdong, YAO Wenbing. Protective effect of fibroin peptides on Aβ25-35-induced injury in SH-SY5Y cells and its mechanism[J]. Journal of China Pharmaceutical University, 2017, 48(5): 609-613. DOI: 10.11665/j.issn.1000-5048.20170517
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Protective effect of fibroin peptides on Aβ25-35-induced injury in SH-SY5Y cells and its mechanism

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    • Abstract
  • This study aimed at exploring the molecular mechanism of fibroin peptide in preventing the Aβ25-35-induced neuronal damages in SH-SY5Y cells. MTT was used to detect the effect of fibroin peptide on the changes of the Aβ25-35-induced injuries in SH-SY5Y cells; Western blot was employed to detect the effect of fibroin peptide on the changes of the Aβ25-35-induced hyperphosphorylation of Tau in SH-SY5Y cells; DCFH-DA probe method was used to detect the effect of fibroin peptide on the Aβ-induced production of intracellular reactive oxygen species(ROS)in SH-SY5Y cells. The results indicated that fibroin peptide could improve the activity of the PP2A and inhibit the activity of GSK-3β to decrease the hyperphosphorylation of Tau induced by Aβ25-35. Fibroin peptide could significantly prevent the Aβ25-35-induced neuronal damages and multisite Tau hyperphosphorylation. In addition, fibroin peptide could also reduce oxidative damage to protect neurons by significantly decreasing the Aβ25-35-induced production of intracellular ROS.
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