Effect of triterpenic acid-enriched fraction from<i> Cyclocarya paliurus</i> on high glucose-induced pancreatic α cells insulin resistance

WANG Yiting; ZHAO Mengge; SHENG Xueping; ZHANG Jian; JIANG Cuihua; YIN Zhiqi

doi:10.11665/j.issn.1000-5048.20180212

Journal of China Pharmaceutical University > 2018 > 49(2): 215-221. > DOI: 10.11665/j.issn.1000-5048.20180212

WANG Yiting, ZHAO Mengge, SHENG Xueping, ZHANG Jian, JIANG Cuihua, YIN Zhiqi. Effect of triterpenic acid-enriched fraction from Cyclocarya paliurus on high glucose-induced pancreatic α cells insulin resistance[J]. Journal of China Pharmaceutical University, 2018, 49(2): 215-221. DOI: 10.11665/j.issn.1000-5048.20180212

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Effect of triterpenic acid-enriched fraction from Cyclocarya paliurus on high glucose-induced pancreatic α cells insulin resistance

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To investigate the effect and possible mechanisms of triterpenic acid-enriched fraction from Cyclocarya paliurus(TAE)on glucagon secretion in insulin-resistance pancreatic α cells, the model of insulin resistance in αTC1-6 cells was induced by long term exposure to high glucose. Experimental groups were divided as follow: control(5. 5 mmol/L glucose), model(25 mmol/L), TAE(1, 5, 10 μg/mL), and TAE(10 μg/mL)plus wortmannin(10 nmol/L)group. The supernatant and lysate of treated cells were collected to determine glucagon secretion by ELISA kit. The mRNA and protein abundance of IRS-1, PI3K and Akt were measured by qPCR and Western blot analysis. Results showed that TAE could not only significantly reduce glucagon secretion induce by high glucose in a dose-dependent manner, but also remarkably increased the mRNA and protein abundance of IRS-1, PI3K and Akt in αTC1-6 cells. However, these effects of TAE were reversed by PI3K inhibitor wortmannin. In conclusion, it suggested that TAE could improve the insulin resistance induced by high glucose in pancreatic α cells which may be related with the activation of IRS-1/PI3K/Akt signaling pathway.
- Cyclocarya paliurus,
- triterpenic acid-enriched(TAE),
- αTC1-6 cells,
- insulin resistance,
- type 2 diabetes

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