Effect of triterpenic acid-enriched fraction from<i> Cyclocarya paliurus</i> on high glucose-induced pancreatic α cells insulin resistance

WANG Yiting; ZHAO Mengge; SHENG Xueping; ZHANG Jian; JIANG Cuihua; YIN Zhiqi

doi:10.11665/j.issn.1000-5048.20180212

Journal of China Pharmaceutical University > 2018 > 49(2): 215-221. > DOI: 10.11665/j.issn.1000-5048.20180212

WANG Yiting, ZHAO Mengge, SHENG Xueping, ZHANG Jian, JIANG Cuihua, YIN Zhiqi. Effect of triterpenic acid-enriched fraction from Cyclocarya paliurus on high glucose-induced pancreatic α cells insulin resistance[J]. Journal of China Pharmaceutical University, 2018, 49(2): 215-221. DOI: 10.11665/j.issn.1000-5048.20180212

Citation:

PDF (2077 KB)

Effect of triterpenic acid-enriched fraction from Cyclocarya paliurus on high glucose-induced pancreatic α cells insulin resistance

More Information

Graphical Abstract

Abstract

Abstract

To investigate the effect and possible mechanisms of triterpenic acid-enriched fraction from Cyclocarya paliurus(TAE)on glucagon secretion in insulin-resistance pancreatic α cells, the model of insulin resistance in αTC1-6 cells was induced by long term exposure to high glucose. Experimental groups were divided as follow: control(5. 5 mmol/L glucose), model(25 mmol/L), TAE(1, 5, 10 μg/mL), and TAE(10 μg/mL)plus wortmannin(10 nmol/L)group. The supernatant and lysate of treated cells were collected to determine glucagon secretion by ELISA kit. The mRNA and protein abundance of IRS-1, PI3K and Akt were measured by qPCR and Western blot analysis. Results showed that TAE could not only significantly reduce glucagon secretion induce by high glucose in a dose-dependent manner, but also remarkably increased the mRNA and protein abundance of IRS-1, PI3K and Akt in αTC1-6 cells. However, these effects of TAE were reversed by PI3K inhibitor wortmannin. In conclusion, it suggested that TAE could improve the insulin resistance induced by high glucose in pancreatic α cells which may be related with the activation of IRS-1/PI3K/Akt signaling pathway.
- Cyclocarya paliurus,
- triterpenic acid-enriched(TAE),
- αTC1-6 cells,
- insulin resistance,
- type 2 diabetes

FullText(HTML)

References (25)

References

[1]	Heller A,Feldman B.Electrochemistry in diabetes management[J].Account Chem Res,2010,43(7):963-973.
[2]	Organization WH.Global report on diabetes[J].Working Papers,2016.
[3]	Unger R,Orci L.The essential role of glucagon in the pathogenesis of diabetes mellitus[J].Lancet,1975,1(7897):14-16.
[4]	Gosmain Y,Masson MH,Philippe J.Glucagon:the renewal of an old hormone in the pathophysiology of diabetes[J].J Diabetes,2013,5(2):102-109.
[5]	Unger RH, Cherrington AD. Glucagonocentric restructuring of diabetes:a pathophysiologic and therapeutic makeover[J].J Clin Invest,2012,122(1):4-12.
[6]	Tsuchiyama N,Takamura T,Ando H,et al.Possible role of alpha-cell insulin resistance in exaggerated glucagon responses to arginine in type 2 diabetes[J].Diabetes Care,2007,30(30):2583-2587.
[7]	Hong J,Jeppesen PB,Hermansen K.Effects of elevated fatty acid and glucose concentrations on pancreatic islet function in vitro[J].Diabetes Obes Metab,2010,11(4):397-404.
[8]	Wang QQ,Jiang CH,Fang SZ,et al.Antihyperglycemic,antihyperlipidemic and antioxidant effects of ethanol and aqueous extracts of Cyclocarya paliurus leaves in type 2 diabetic rats[J].J Ethnopharmacol,2013,150(3):1119-1127.
[9]	Fan BD,Wei Y,Li CH,et al.Research progress in chemical constituents and hpyerglycemic activity of Cyclocarya paliurus[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2014,20(13):239-242.
[10]	Kurihara H,Fukami H,Kusumoto A,et al.Hypoglycemic action of Cyclocarya paliurus(Batal.)Iljinskaja in normal and diabetic mice[J].J Agric Chem Soc Japan,2003,67(4):877-880.
[11]	Li TT,Wu CY,Fang SZ,et al.Content stability and hypoglycemic effect of Cyclocarya paliurus capsule[J].Sci Technol Food Ind(食品工业科技),2013,34(19):337-340.
[12]	Xu G,Yoshitomi H,Wen S,et al.Cyclocarya paliurus(Batal.)Ijinskaja aqueous extract(CPAE)ameliorates obesity by improving insulin signaling in the hypothalamus of a metabolic syndrome rat model[J].Evid-Based Compl Alt,2017,2017(2):4602153.
[13]	Zhu KN,Jiang CH,Tian YS,et al.Two triterpeniods from Cyclocarya paliurus(Batal)Iljinsk(Juglandaceae)promote glucose uptake in 3T3-L1 adipocytes:the relationship to AMPK activation[J].Phytomedicine,2015,22(9):837-846.
[14]	Shen XX,Li HL,Pan L,et al.Glucotoxicity and cell dysfunction:involvement of the PI3K/Akt pathway in glucose-induced insulin resistance in rat islets and clonal TC1-6 cells[J].Endocr Res,2012,37(1):12-24.
[15]	Godsland IF, Walton C, Wynn V. Role of glucose and insulin resistance in development of type 2 diabetes mellitus[J].Lancet,1992,340(8831):1347-1348.
[16]	Kisanuki K,Kishikawa H,Araki E,et al.Expression of insulin receptor on clonal pancreatic alpha cells and its possible role for insulin-stimulated negative regulation of glucagon secretion[J].Diabetologia,1995,38(4):422-429.
[17]	Mizusawa N,Hasegawa T,Ohigashi I,et al.Differentiation phenotypes of pancreatic islet beta- and alpha-cells are closely related with homeotic genes and a group of differentially expressed genes[J].Gene,2004,331(1):53-63.
[18]	Piro S,Maniscalchi ET,Monello A,et al.Palmitate affects insulin receptor phosphorylation and intracellular insulin signal in a pancreatic alpha-cell line[J].Endocrinology,2010,151(9):4197-4206.
[19]	Chen X,Hermansen K,Xiao J,et al.Isosteviol has beneficial effects on palmitate-induced α-cell dysfunction and gene expression[J].PLoS One,2012,7(3):e34361.
[20]	Mcgirr R,Ejbick CE,Carter DE,et al.Glucose dependence of the regulated secretory pathway in alphaTC1-6 cells[J].Endocrinology,2005,146(10):4514-4523.
[21]	Unger RH.The banting memorial lecture 1975.Diabetes and the alpha cell[J].Diabetes,1976,25(2):136-151.
[22]	Umpaichitra V,Bastian W,Taha D,et al.C-peptide and glucagon profiles in minority children with type 2 diabetes mellitus[J].J Clin Endocr Metab,2001,86(4):1605-1609.
[23]	Ferrannini E,Muscelli E,Natali A,et al.Association of fasting glucagon and proinsulin concentrations with insulin resistance[J].Diabetologia,2007,50(11):2342-2347.
[24]	Diao JY,Asghar Z,Chan CB.Glucose-regulated glucagon secretion requires insulin receptor expression in pancreatic α-cells[J].J Biol Chem,2005,280(39):487-496.
[25]	Kaneko K,Shirotani T,Araki E,et al.Insulin inhibits glucagon secretion by the activation of PI3-kinase in In-R1-G9 cells[J].Diabetes Res Clin Pr,1999,44(2):83-92.

Cited By

Get Citation

PDF

XML

Article views (966) PDF downloads (1472)

Turn off MathJax

Article Contents

Abstract

References

Effect of triterpenic acid-enriched fraction from Cyclocarya paliurus on high glucose-induced pancreatic α cells insulin resistance

Abstract

References

Catalog

Export File

Citation

Format

Content