Inhibition of Potentilla fruticosa extracts on α-amylase, α-glucosidase and aldose reductase

LI Meihua; WANG Weiqing; ZENG Yang; GUO Fengxia; YAN Peiying; LI Jinping

doi:10.11665/j.issn.1000-5048.20180413

Journal of China Pharmaceutical University > 2018 > 49(4): 470-475. > DOI: 10.11665/j.issn.1000-5048.20180413

LI Meihua, WANG Weiqing, ZENG Yang, GUO Fengxia, YAN Peiying, LI Jinping. Inhibition of Potentilla fruticosa extracts on α-amylase, α-glucosidase and aldose reductase[J]. Journal of China Pharmaceutical University, 2018, 49(4): 470-475. DOI: 10.11665/j.issn.1000-5048.20180413

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Inhibition of Potentilla fruticosa extracts on α-amylase, α-glucosidase and aldose reductase

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Abstract

This study measured the in vitro inhibitory effects of α-amylase(AM), α-glycosidase(AG)and aldose reductase(AR)extraction from Potentilla fruticosa in three solvents: water extract(WE)and 95% methanol extraction of petroleum ether part(MEP), 95% methanol extraction of ethyl acetate part(MEE)and 95% methanol extraction of water part(MEW)through α-amylase inhibitors(AMI), α-glycosidase inhibitors(AGI)and aldose reductase inhibitors(ARI)activity screening models. In vivo effects of different solvents from Potentilla fruticosa on impaired glucose tolerance of mice were also measured. Among them, WE, MEP and MEE exhibited against AMI activity with IC₅₀ values of 0. 432, 1. 193 and 0. 507 mg/mL, respectively. Three solvents against AGI activity with IC₅₀ values of 0. 164, 0. 768 and 0. 466 mg/mL, respectively. Three solvents against ARI activity with IC₅₀ values of 0. 742, 2. 158 and 1. 098 mg/mL, respectively. The study suggests that Potentilla fruticosa in water extract and 95% methanol extraction of ethyl acetate part demonstrated a stronger inhibitory effect on AM, AG and AR. Meanwhile, Potentilla fruticosa in water extract and 95% methanol extraction of ethyl acetate part can be significantly decreased the postprandial blood glucose in mice.
- Potentilla fruticosa,
- extract,
- α-amylase,
- α-glucosidase,
- aldose reductase,
- postprandial blood glucose

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Inhibition of Potentilla fruticosa extracts on α-amylase, α-glucosidase and aldose reductase

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