• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
ZOU Fuxian, XU Wen, HUANG Zehao, ZHANG Xun, CHEN Shuyun, LIN Yu, XU Wei. Analysis of transcriptome sequencing and related genes of flavonoid biosynthesis from Anoectochilus roxburghii[J]. Journal of China Pharmaceutical University, 2019, 50(1): 66-74. DOI: 10.11665/j.issn.1000-5048.20190109
Citation: ZOU Fuxian, XU Wen, HUANG Zehao, ZHANG Xun, CHEN Shuyun, LIN Yu, XU Wei. Analysis of transcriptome sequencing and related genes of flavonoid biosynthesis from Anoectochilus roxburghii[J]. Journal of China Pharmaceutical University, 2019, 50(1): 66-74. DOI: 10.11665/j.issn.1000-5048.20190109

Analysis of transcriptome sequencing and related genes of flavonoid biosynthesis from Anoectochilus roxburghii

More Information
  • Transcriptome sequencing was performed for the first time on Anoectochilus roxburghii(AR)in different harvesting periods using RNA-seq high-throughput sequencing technique, and the results were verified and analyzed by Q-PCR and HPLC. A total of 51, 370 genes were obtained by transcriptome sequencing and annotated to the database of Nr, GO, Swiss-Prot, KEGG and KOG. The species that were sequenced according to the homology sequence were the same as AR monocotyledon plants. Through comparison of AR transcriptome in different periods, it was found that the differences were mainly in flavonoid biosynthesis-related genes. The expression levels of flavonoid biosynthesis-related genes(trans-cinnamate 4-monooxygenase, caffeoyl-CoA O-methyltransferase, chalcone synthase, flavonol synthase, shikimate O-hydroxycinnamoyltransferase and flavonoid 3′, 5′-hydroxylase)were verified by Q-PCR, and the results were consistent with those of transcriptome sequencing. The contents of 6 flavonoids(rutin, isoquercitrin, narcissin, quercetin, kaempferol and isorhamnrtin)were determined by HPLC. The results showed that the expression of flavonoid synthetic gene in AR increased with the growth time, and the variation trend of flavonoid compound content and gene expression were basically consistent. Combined with transcriptome data, the biosynthetic pathway of flavonoid content in AR was plotted. This study provides important genetic resources for the key genes of flavonoid synthesis in AR and the biosynthesis of flavonoids, as well as the basis for the development of its medicinal value.
  • [1]
    Fujian food and drug administration.Standards of Chinese herbal medicines in Fujian(福建省中药材标准)[M].Fuzhou:Haifeng press(海风出版社),2006:154.
    [2]
    Chinese academy of science flora of China Editorial Board.Flora of China,Vol.17(中国植物志)[M].Beijing:Science Press(科学出版社),1998:220.
    [3]
    Ye S,Shao Q,Zhang A.Anoectochilus roxburghii:A review of its phytochemistry,pharmacology,and clinical applications[J].J Ethnopharmacol,2017,39(209):184-202.
    [4]
    Shen YM,Huang CQ,Liu ZY,et al.Study on quality standard of imitation ecological planting Anoectochilus roxburghii under forest covering[J].China Tradit Herb Drugs(中草药),2018,49(02):450-454.
    [5]
    Zhang ZM,Lu JG,Xie QS,et al.Establishment of fingerprint and determination of kinsenoside of herba anoectochili from different sources by UHPLC-TOF-MS [J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2018,24(01):31-37.
    [6]
    Liu Z,Zhang J,Liu Q,et al.The vascular protective effects of Anoectochilus roxburghii polysaccharose under high glucose conditions[J].J Ethnopharmacol,2017, 202:192-199.
    [7]
    Li L,Li Y,Liu Z,et al.The renal protective effects of Anoectochilus roxburghii polysaccharose on diabetic mice induced by high-fat diet and streptozotocin[J].J Ethnopharmacol,2016, 178:58-65.
    [8]
    Zeng B,Su M,Chen Q,et al.Protective effect of a polysaccharide from Anoectochilus roxburghii against carbon tetrachloride-induced acute liver injury in mice[J].J Ethnopharmacol,2017, 200:124-135.
    [9]
    Jia Y,Zhang FS,Xiao SX,et al.Component Analyses of Tussilago farfara in Different Development Stages by Metabonomic and Comparative Transcriptomic Approaches [J].Chin J Biochem Mol Biol(中国生物化学与分子生物学报),2017,33(6):615-623.
    [10]
    Grabherr MG,Haas BJ,Yassour M,et al.Full-length transcriptome assembly from RNA-Seq data without a reference genome[J].Nat Biotechnol,2011,29(7):644.
    [11]
    Iseli C,Jongeneel CV,Bucher P.ESTScan:a program for detecting,evaluating,and reconstructing potential coding regions in EST sequences.[J].Proc Int Conf Intell Syst Mol Biol,1998,99(2):138-148.
    [12]
    Conesa A,Götz S,Garcíagómez JM,et al.Blast2GO:a universal tool for annotation,visualization and analysis in functional genomics research[J].Bioinformatics,2005,21(18):3674-3676.
    [13]
    Ye J,Fang L,Zheng H,et al.WEGO:a web tool for plotting GO annotations[J].Nucleic Acids Res,2006,34(SI):W293-W297.
    [14]
    Benjamini Y,Hochberg Y.Controlling the false discovery rate-A practical and powerful approach to multiple testing[J].J Roy Stat Soc,1995,57(1):289-300.
    [15]
    Chen Y,Huang JF,Lin N,et al.Simultaneous determination of six constituents in Anoectochilus roxburghii by HPLC [J].Chin Tradit Pat Med(中成药),2018,40(10):2222-2227.
    [16]
    Li R,Fan W,Tian G,et al.The sequence and de novo assembly of the giant panda genome[J].Nature,2010,463(7279):311-317.
    [17]
    Jiang FX,Wei PW,Kou YP,et al.Study on the Characterization of the transcriptome of Anoectochilus formosanus Taiwan[J].Mol Plant Breeding(分子植物育种),2015,13(12):2743-2753.
    [18]
    Zhang G,Zhao M,Song C,et al.Characterization of reference genes for quantitative real-time PCR analysis in various tissues of Anoectochilus roxburghii[J].Mol Biol Rep,2012,39(5):5905-5912.
    [19]
    Zhang JE,Deng HF.Analysis of transcriptome sequencing and qRT-PCR analysis of related genes of flavonoid biosynthesis from Eucommia ulmoides calli[J].Chin Tradit Herbal Drugs(中草药),2018,49(16):3912-3917.
    [20]
    Sun ML.Gene expression of catechins biosynthesis in tea plant [Camellia sinensis(L.)O.Kuntze] with real time fluorescence quantitative PCR(茶儿茶素生物合成相关基因表达的实时荧光定量PCR分析)[D].Anhui:Anhui Agricultural University(安徽农业大学),2010.
  • Related Articles

    [1]HU Linlin, GUO Nan, ZHANG Xueli, SHAO Hua. Determination of daptomycin by UPLC-MS/MS and its pharmacokinetic eva-luation in critically ill patients[J]. Journal of China Pharmaceutical University, 2015, 46(6): 700-706. DOI: 10.11665/j.issn.1000-5048.20150611
    [2]HAO Kun, YU Dan, WANG Guangji. Pharmacokinetic translational research from bench to bedside[J]. Journal of China Pharmaceutical University, 2015, 46(1): 50-57. DOI: 10.11665/j.issn.1000-5048.20150105
    [3]LIANG Yan, XING Rong, LIU Jiali, MAO Yong, FU Hanxu, XIE Lin, WANG Guangji. Advances of novel technologies and theories in pharmacokinetic research[J]. Journal of China Pharmaceutical University, 2014, 45(6): 607-616. DOI: 10.11665/j.issn.1000-5048.20140601
    [4]Application of pharmacometrics in drug development and therapeutic drug monitoring[J]. Journal of China Pharmaceutical University, 2010, 41(1): 91-96.
    [5]Pharmacokinetics Studies of 99mTc-BM[J]. Journal of China Pharmaceutical University, 2004, (3): 57-59.
    [6]Pharmacokinetics of 0325k1-1 in Dogs[J]. Journal of China Pharmaceutical University, 2003, (4): 79-81.
    [7]Pharmacokinetics of Aceclofenac in Rats[J]. Journal of China Pharmaceutical University, 1998, (4): 68-70.
    [8]Pharmacokinetics and Pharmacodynamics of Sophocarpine and Oxysophocarpine[J]. Journal of China Pharmaceutical University, 1992, (3): 161-164.
    [9]PHARMACOKINETICS OF MATRINR IN RABBITS[J]. Journal of China Pharmaceutical University, 1986, (4): 309-311.
    [10]PHARMACOKINETICS OF OCTANOYLPRIMAQUINE IN RABBITS[J]. Journal of China Pharmaceutical University, 1986, (2): 125-128.

Catalog

    Article views (769) PDF downloads (1192) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return