Qualitative and quantitative analyses of Chrysanthemum morifolium ‘Fubaiju’ by LC-MSⁿ and HPLC-UV

A qualitative analytical method of liquid chromatography coupled with mass spectrometry(LC-MSⁿ)was developed for the identification of main constituents in Chrysanthemum morifolium ‘Fubaiju’. High-performance liquid chromatography(HPLC)was developed for the quantification of five active components, including chlorogenic acid( 1 ), luteolin-7-O-β-D-glucopyranoside( 2 ), luteolin-7-O-β-D-glucopyranuronide( 3 ), 3, 5-Di-caffeoylquinic acid( 4 ), and apigenin-7-O-β-D-glucopyranoside( 5 ). A total of 22 compounds, including 13 flavonoids and 9 phenolic acids, were identified based on their retention behaviors, UV profiles and MS fragment information. Furthermore, a validation method with good linearity(r> 0. 999 9), precision, stability, repeatability and recovery was successfully applied for simultaneous determination of five major components in 10 batches of C. morifolium ‘Fubaiju’ by HPLC-UV method. The established method was proved to be a validation strategy for the quality evaluation of C. morifolium ‘Fubaiju’.