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WANG Xiaoying, WANG Xiaying, QIU Liangzhen, OUYANG Huizhi, XU Wei. Cytotoxicity and cellular uptake of paclitaxel-loaded carboxymethyl chitosan-rhein polymeric micelles in MCF-7 cells[J]. Journal of China Pharmaceutical University, 2020, 51(1): 33-37. DOI: 10.11665/j.issn.1000-5048.20200106
Citation: WANG Xiaoying, WANG Xiaying, QIU Liangzhen, OUYANG Huizhi, XU Wei. Cytotoxicity and cellular uptake of paclitaxel-loaded carboxymethyl chitosan-rhein polymeric micelles in MCF-7 cells[J]. Journal of China Pharmaceutical University, 2020, 51(1): 33-37. DOI: 10.11665/j.issn.1000-5048.20200106

Cytotoxicity and cellular uptake of paclitaxel-loaded carboxymethyl chitosan-rhein polymeric micelles in MCF-7 cells

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  • In this study, in vitro cytotoxicity of carboxymethyl chitosan-rhein conjugate(CR conjugate)and paclitaxel-loaded carboxymethyl chitosan-rhein polymeric micelles(PTX/CR PMs)was evaluated by MTT method in MCF-7 cells. The results showed that CR conjugate displayed good security; PTX/CR PMs in 24 h showed better antitumor activity than Taxol® . Environment-responsive fluorescent probe P4 was used to determine the cellular uptake of PTX/CR PMs in MCF-7 cells. The results also showed that P4 and PTX co-loaded carboxymethyl chitosan-rhein polymeric micelles [(P4+PTX)/CR PMs] could be taken up by MCF-7 cells. There was no difference between(P4+PTX)/CR PMs group and(P4+PTX)/CR PMs with verapamil group, suggesting that CR PMs could protect fluorescent probe and/or drugs in their cores avoiding efflux by P-glycoprotein. These results will contribute to in vivo study of CR conjugate and PTX/CR PMs in the future.
  • [1]
    Wang XY,Qiu LZ,Li QZ,et al.Synthesis of PEGylated carboxymethyl chitosan-rhein conjugate and preparation of paclitaxel-loaded polymeric micelles[J].J China Pharm Univ(中国药科大学学报),2018,49(5):596-602.
    [2]
    Sun JF,Guo CJ,Zhang YD,et al.Preparation and characterization of multi-functional targeting nano-carier based on pH-redox tumor microenvironment[J].J China Pharm Univ(中国药科大学学报),2017,48(3):305-310.
    [3]
    Wang XY,Chen YH,Dahmani FZ,et al.Amphiphilic carboxymethyl chitosan-quercetin conjugate with P-gp inhibitory properties for oral delivery of paclitaxel[J].Biomaterials,2014,35(26):7654-7665.
    [4]
    Wang XY,Guo YL,Qiu LZ,et al.Preparation and evaluation of carboxymethyl chitosan-rhein polymeric micelles with synergistic antitumor effect for oral delivery of paclitaxel[J].Carbohydr Polym,2019,206:121-131.
    [5]
    Guo YL,Qiu LZ,Lin X,et al.Preparation of paclitaxel loaded carboxymethyl chitosan-rhein polymeric micelles[J].Fujian J Tradit Chin Med(福建中医药),2017,48(1):16-18.
    [6]
    Bernabeu E,Gonzalez L,Cagel M,et al.Novel soluplus(+)-TPGS mixed micelles for encapsulation of paclitaxel with enhanced in vitro cytotoxicity on breast and ovarian cancer cell lines[J].Colloids Surf B Biointerfaces,2016,140:403-411.
    [7]
    Ahmed AA,Wang XY,Lu Z,et al.Modulating microtubule stability enhances the cytotoxic response of cancer cells to Paclitaxel[J].Cancer Res,2011,71(17):5806-5817.
    [8]
    Hu XW, Zhang J, Yu Z, et al. Environment-responsive aza-BODIPY dyes quenching in water as potential probes to visualize the in vivo fate of lipid-based nanocarriers[J].Nanomed-Nanotechnol Biol Med,2015,11(8):1939-1948.
    [9]
    Ma YH,He HS,Xia F,et al.In vivo fate of lipid-silybin conjugate nanoparticles:implications on enhanced oral bioavailability[J].Nanomed-Nanotechnol Biol Med,2017,13(8):2643-2654.
    [10]
    Xie YK,Shi BK,Xia F,et al.Epithelia transmembrane transport of orally administered ultrafine drug particles evidenced by environment sensitive fluorophores in cellular and animal studies[J].J Control Release,2018,270:65-75.
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