Cytotoxicity and cellular uptake of paclitaxel-loaded carboxymethyl chitosan-rhein polymeric micelles in MCF-7 cells

WANG Xiaoying; WANG Xiaying; QIU Liangzhen; OUYANG Huizhi; XU Wei

doi:10.11665/j.issn.1000-5048.20200106

Journal of China Pharmaceutical University > 2020 > 51(1): 33-37. > DOI: 10.11665/j.issn.1000-5048.20200106

WANG Xiaoying, WANG Xiaying, QIU Liangzhen, OUYANG Huizhi, XU Wei. Cytotoxicity and cellular uptake of paclitaxel-loaded carboxymethyl chitosan-rhein polymeric micelles in MCF-7 cells[J]. Journal of China Pharmaceutical University, 2020, 51(1): 33-37. DOI: 10.11665/j.issn.1000-5048.20200106

Citation:

PDF (2424 KB)

Cytotoxicity and cellular uptake of paclitaxel-loaded carboxymethyl chitosan-rhein polymeric micelles in MCF-7 cells

More Information

Graphical Abstract

Abstract

Abstract

In this study, in vitro cytotoxicity of carboxymethyl chitosan-rhein conjugate(CR conjugate)and paclitaxel-loaded carboxymethyl chitosan-rhein polymeric micelles(PTX/CR PMs)was evaluated by MTT method in MCF-7 cells. The results showed that CR conjugate displayed good security; PTX/CR PMs in 24 h showed better antitumor activity than Taxol^®. Environment-responsive fluorescent probe P4 was used to determine the cellular uptake of PTX/CR PMs in MCF-7 cells. The results also showed that P4 and PTX co-loaded carboxymethyl chitosan-rhein polymeric micelles ［(P4+PTX)/CR PMs］ could be taken up by MCF-7 cells. There was no difference between(P4+PTX)/CR PMs group and(P4+PTX)/CR PMs with verapamil group, suggesting that CR PMs could protect fluorescent probe and/or drugs in their cores avoiding efflux by P-glycoprotein. These results will contribute to in vivo study of CR conjugate and PTX/CR PMs in the future.
- paclitaxel,
- carboxymethyl chitosan-rhein conjugate,
- polymeric micelles,
- anti-tumor,
- cellular uptake

FullText(HTML)

References (10)

References

[1]	Wang XY,Qiu LZ,Li QZ,et al.Synthesis of PEGylated carboxymethyl chitosan-rhein conjugate and preparation of paclitaxel-loaded polymeric micelles[J].J China Pharm Univ(中国药科大学学报),2018,49(5):596-602.
[2]	Sun JF,Guo CJ,Zhang YD,et al.Preparation and characterization of multi-functional targeting nano-carier based on pH-redox tumor microenvironment[J].J China Pharm Univ(中国药科大学学报),2017,48(3):305-310.
[3]	Wang XY,Chen YH,Dahmani FZ,et al.Amphiphilic carboxymethyl chitosan-quercetin conjugate with P-gp inhibitory properties for oral delivery of paclitaxel[J].Biomaterials,2014,35(26):7654-7665.
[4]	Wang XY,Guo YL,Qiu LZ,et al.Preparation and evaluation of carboxymethyl chitosan-rhein polymeric micelles with synergistic antitumor effect for oral delivery of paclitaxel[J].Carbohydr Polym,2019,206:121-131.
[5]	Guo YL,Qiu LZ,Lin X,et al.Preparation of paclitaxel loaded carboxymethyl chitosan-rhein polymeric micelles[J].Fujian J Tradit Chin Med(福建中医药),2017,48(1):16-18.
[6]	Bernabeu E,Gonzalez L,Cagel M,et al.Novel soluplus(+)-TPGS mixed micelles for encapsulation of paclitaxel with enhanced in vitro cytotoxicity on breast and ovarian cancer cell lines[J].Colloids Surf B Biointerfaces,2016,140:403-411.
[7]	Ahmed AA,Wang XY,Lu Z,et al.Modulating microtubule stability enhances the cytotoxic response of cancer cells to Paclitaxel[J].Cancer Res,2011,71(17):5806-5817.
[8]	Hu XW, Zhang J, Yu Z, et al. Environment-responsive aza-BODIPY dyes quenching in water as potential probes to visualize the in vivo fate of lipid-based nanocarriers[J].Nanomed-Nanotechnol Biol Med,2015,11(8):1939-1948.
[9]	Ma YH,He HS,Xia F,et al.In vivo fate of lipid-silybin conjugate nanoparticles:implications on enhanced oral bioavailability[J].Nanomed-Nanotechnol Biol Med,2017,13(8):2643-2654.
[10]	Xie YK,Shi BK,Xia F,et al.Epithelia transmembrane transport of orally administered ultrafine drug particles evidenced by environment sensitive fluorophores in cellular and animal studies[J].J Control Release,2018,270:65-75.

Cited By

Get Citation

PDF

XML

Article views (361) PDF downloads (807)

Turn off MathJax

Article Contents

Abstract

References

Cytotoxicity and cellular uptake of paclitaxel-loaded carboxymethyl chitosan-rhein polymeric micelles in MCF-7 cells

Abstract

References

Catalog

Export File

Citation

Format

Content