• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
WANG Xiaoying, WANG Xiaying, QIU Liangzhen, OUYANG Huizhi, XU Wei. Cytotoxicity and cellular uptake of paclitaxel-loaded carboxymethyl chitosan-rhein polymeric micelles in MCF-7 cells[J]. Journal of China Pharmaceutical University, 2020, 51(1): 33-37. DOI: 10.11665/j.issn.1000-5048.20200106
Citation: WANG Xiaoying, WANG Xiaying, QIU Liangzhen, OUYANG Huizhi, XU Wei. Cytotoxicity and cellular uptake of paclitaxel-loaded carboxymethyl chitosan-rhein polymeric micelles in MCF-7 cells[J]. Journal of China Pharmaceutical University, 2020, 51(1): 33-37. DOI: 10.11665/j.issn.1000-5048.20200106
shu

Cytotoxicity and cellular uptake of paclitaxel-loaded carboxymethyl chitosan-rhein polymeric micelles in MCF-7 cells

More Information
    Graphical Abstract
    • Abstract
  • In this study, in vitro cytotoxicity of carboxymethyl chitosan-rhein conjugate(CR conjugate)and paclitaxel-loaded carboxymethyl chitosan-rhein polymeric micelles(PTX/CR PMs)was evaluated by MTT method in MCF-7 cells. The results showed that CR conjugate displayed good security; PTX/CR PMs in 24 h showed better antitumor activity than Taxol® . Environment-responsive fluorescent probe P4 was used to determine the cellular uptake of PTX/CR PMs in MCF-7 cells. The results also showed that P4 and PTX co-loaded carboxymethyl chitosan-rhein polymeric micelles ［(P4+PTX)/CR PMs］ could be taken up by MCF-7 cells. There was no difference between(P4+PTX)/CR PMs group and(P4+PTX)/CR PMs with verapamil group, suggesting that CR PMs could protect fluorescent probe and/or drugs in their cores avoiding efflux by P-glycoprotein. These results will contribute to in vivo study of CR conjugate and PTX/CR PMs in the future.
    • FullText(HTML)
    • References (10)
  • [1]
    Wang XY,Qiu LZ,Li QZ,et al.Synthesis of PEGylated carboxymethyl chitosan-rhein conjugate and preparation of paclitaxel-loaded polymeric micelles[J].J China Pharm Univ(中国药科大学学报),2018,49(5):596-602.
    [2]
    Sun JF,Guo CJ,Zhang YD,et al.Preparation and characterization of multi-functional targeting nano-carier based on pH-redox tumor microenvironment[J].J China Pharm Univ(中国药科大学学报),2017,48(3):305-310.
    [3]
    Wang XY,Chen YH,Dahmani FZ,et al.Amphiphilic carboxymethyl chitosan-quercetin conjugate with P-gp inhibitory properties for oral delivery of paclitaxel[J].Biomaterials,2014,35(26):7654-7665.
    [4]
    Wang XY,Guo YL,Qiu LZ,et al.Preparation and evaluation of carboxymethyl chitosan-rhein polymeric micelles with synergistic antitumor effect for oral delivery of paclitaxel[J].Carbohydr Polym,2019,206:121-131.
    [5]
    Guo YL,Qiu LZ,Lin X,et al.Preparation of paclitaxel loaded carboxymethyl chitosan-rhein polymeric micelles[J].Fujian J Tradit Chin Med(福建中医药),2017,48(1):16-18.
    [6]
    Bernabeu E,Gonzalez L,Cagel M,et al.Novel soluplus(+)-TPGS mixed micelles for encapsulation of paclitaxel with enhanced in vitro cytotoxicity on breast and ovarian cancer cell lines[J].Colloids Surf B Biointerfaces,2016,140:403-411.
    [7]
    Ahmed AA,Wang XY,Lu Z,et al.Modulating microtubule stability enhances the cytotoxic response of cancer cells to Paclitaxel[J].Cancer Res,2011,71(17):5806-5817.
    [8]
    Hu XW, Zhang J, Yu Z, et al. Environment-responsive aza-BODIPY dyes quenching in water as potential probes to visualize the in vivo fate of lipid-based nanocarriers[J].Nanomed-Nanotechnol Biol Med,2015,11(8):1939-1948.
    [9]
    Ma YH,He HS,Xia F,et al.In vivo fate of lipid-silybin conjugate nanoparticles:implications on enhanced oral bioavailability[J].Nanomed-Nanotechnol Biol Med,2017,13(8):2643-2654.
    [10]
    Xie YK,Shi BK,Xia F,et al.Epithelia transmembrane transport of orally administered ultrafine drug particles evidenced by environment sensitive fluorophores in cellular and animal studies[J].J Control Release,2018,270:65-75.
    • Related Articles

  • Related Articles

    [1]LIANG Jinlai, XU Wei, YIN Tingjie, HUO Meirong. Advances in the hypoxia-responsive antitumor drug nanocarriers and tumor hypoxia relieve[J]. Journal of China Pharmaceutical University, 2018, 49(3): 255-262. DOI: 10.11665/j.issn.1000-5048.20180301
    [2]GUO Yahui, LU Peng, WANG Yubin, ZHANG Huibin. Progress in the researches for antitumor NEDD8 activating enzyme inhibitors[J]. Journal of China Pharmaceutical University, 2017, 48(6): 646-653. DOI: 10.11665/j.issn.1000-5048.20170603
    [3]GAO Yahan, PING Qineng, ZONG Li. Preparation and antitumor activity of mitoxantrone conjugated D-α-tocopherylpolyethylene glycol 1000 succinate prodrug micelle[J]. Journal of China Pharmaceutical University, 2016, 47(3): 311-316. DOI: 10.11665/j.issn.1000-5048.20160311
    [4]LIU Yan, WANG Tingting, CHEN Li. Synthesis and antitumor activity of isosteviol derivatives[J]. Journal of China Pharmaceutical University, 2016, 47(1): 48-53. DOI: 10.11665/j.issn.1000-5048.20160106
    [5]LIU Wenhu, CHANG Jinxia, ZHANG Jianwu. Synthesis and antitumor activities of novel coumarin derivatives containing chalcone moiety[J]. Journal of China Pharmaceutical University, 2014, 45(5): 522-528. DOI: 10.11665/j.issn.1000-5048.20140503
    [6]CHENG Haibo, XU Bin, ZHANG Huibin, ZHOU Jinpei. Synthetic process of antitumor drug dacomitinib[J]. Journal of China Pharmaceutical University, 2014, 45(2): 165-169. DOI: 10.11665/j.issn.1000-5048.20140206
    [7]LI Linhu, CHEN Li, XIA Yufeng. Progress in the study of coumarin derivatives as antitumor agents[J]. Journal of China Pharmaceutical University, 2013, 44(4): 374-379. DOI: 10.11665/j.issn.1000-5048.20130417
    [8]JIN Hui, LIU Lifeng, DENG Weiping, LIU Jianwen. Antitumor effects and molecular mechanisms of novel selective HDAC inhibitors in human cervix carcinoma HeLa cells[J]. Journal of China Pharmaceutical University, 2013, 44(4): 352-356. DOI: 10.11665/j.issn.1000-5048.20130413
    [9]Cooperative Effects of Monkshood Polysaccharide with Adriamycin Magnetic Albumin Microsphere Targeting Therapy on Tumor[J]. Journal of China Pharmaceutical University, 2003, (6): 68-72.
    [10]Studies on the Difference in Content of an Antitumor Component 5F in Pteris semipinnata L.[J]. Journal of China Pharmaceutical University, 2002, (5): 48-50.

  • Cited by

    Periodical cited type(2)

    1. 李焱琳，王琪. 干扰素介导肿瘤免疫分子机制的相关研究进展. 临床合理用药. 2025(02): 177-180 .
    2. 梅家豪，洪泽，王琛. 靶向cGAS-STING信号通路药物的研究进展. 中国药科大学学报. 2020(03): 249-259 . 本站查看

    Other cited types(0)

Catalog

    Get Citation
    PDF
    XML
    Article views (359) PDF downloads (806) Cited by(2)
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles

    Copyright © Journal of China Pharmaceutical University苏ICP备12050101号-2

    Address：24 Tongjia Lane, Nanjing City, Jiangsu Province （210009）Tel: 025-83271566E-mail: xuebao@cpu.edu.cn

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    Total visits：366195

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return