• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
ZHOU Liyu, MA Yuanjing, SUN Yuting, CHEN Weikai, QIU Runze, YUAN Dongping, LONG Jun. Effects of Yangyin Huoxue Prescription on the imbalance of Treg/Th17 and atherosclerosis in ApoE-/- mice induced by high-fat diet[J]. Journal of China Pharmaceutical University, 2020, 51(1): 60-67. DOI: 10.11665/j.issn.1000-5048.20200110
Citation: ZHOU Liyu, MA Yuanjing, SUN Yuting, CHEN Weikai, QIU Runze, YUAN Dongping, LONG Jun. Effects of Yangyin Huoxue Prescription on the imbalance of Treg/Th17 and atherosclerosis in ApoE-/- mice induced by high-fat diet[J]. Journal of China Pharmaceutical University, 2020, 51(1): 60-67. DOI: 10.11665/j.issn.1000-5048.20200110

Effects of Yangyin Huoxue Prescription on the imbalance of Treg/Th17 and atherosclerosis in ApoE-/- mice induced by high-fat diet

More Information
  • Atherosclerosis(AS), characterized with the accumulation of lipids on the vessel wall, is an immune-related inflammatory disease which promotes the progression of cardiovascular diseases(CVD). The imbalance of Treg/Th17 accelerates the progression of AS. Yangyin Huoxue Prescription(YHF)is an efficient traditional Chinese medicine used in the treatment of AS, but the effects of YHF on the balance of immunity have still not been clarified. This project was designed to investigate the effects of YHF on the imbalance of Treg/Th17 and AS in ApoE-/- mice induced by high-fat diet(HFD). ApoE-/- mice were given HFD to induce AS and administered low-dose YHF(18 g/kg)or high-dose YHF(36 g/kg)for 20 weeks. Atherosclerotic plaque area was analyzed by oil red O staining. Serum lipids were measured by biochemical kits. Treg or Th17 cells in peripheral blood were detected by flow cytometry. mRNA and protein expression of Foxp3 and RORγt of aortas were determined by qRT-PCR, Western blot and immunohistochemistry. Splenic CD4+T cells of mice were isolated and activated by anti-CD3/CD28, and then treated with lipopolysaccharide(LPS)and YHF. The expression of mRNA and protein of Foxp3 and RORγt were detected by qRT-PCR and immunofluorescence. It was found that YHF reduced the plaque area, decreased lipid level and increased the ratio of Treg cells in peripheral blood. Moreover, YHF increased mRNA or protein expression of Foxp3 in aortas in vivo or CD4+T cells in vitro while decreasing mRNA or protein expression of RORγt. These results suggested that YHF can regulate the imbalance of Treg/Th17 in ApoE-/- mice induced by HFD, and reduce the inflammatory stimulation of LPS on CD4+T cells, thereby improving AS.
  • [1]
    Fan WX.Research progress on the mechanism of ischemic stroke[J].J China Pharm Univ(中国药科大学学报),2018,49(6):751-759.
    [2]
    Ross R.Atherosclerosis:an inflammatory disease[J].N Engl J Med,1999,340(2):115-126.
    [3]
    Foks AC,Kuiper J.Immune checkpoint proteins:exploring their therapeutic potential to regulate atherosclerosis[J].Br J Pharmacol,2017,174(22):3940-3955.
    [4]
    Ding JW,Zheng XX,Zhou T,et al.HMGB1 modulates the Treg/Th17 ratio in atherosclerotic patients[J].J Atheroscler Thromb,2016,23(6):737-745.
    [5]
    Zhu Y,Huang H,Gu WJ,et al.Clinical effect of luhuang granule in treatment of atherosclerosis:an analysis of 60 cases[J].J Anhui Univ Chin Med(安徽中医药大学学报),2019,38(2):20-25.
    [6]
    He JJ,Ma YJ,Qiu RZ,et al.The effects of Qingre Huoxue Yangyin formula on blood lipid,arterial plaque and Th1/Th2 imbalance[J].J Nanjing Univ Tradit Chin Med(南京中医药大学学报),2017,33:623-626.
    [7]
    Ma YJ,Qiu RZ,Yuan DP,et al.Effects of YHF on expression of treg in apolipoprotein E knockout mice[J].Tradit Chin Drug Res Clin Pharmacol(中药新药与临床药理),2018,29(3):313-318.
    [8]
    Qiu RZ,Long J,Zhou LY,et al.Yangyin Qingre Huoxue method in traditional Chinese medicine ameliorates atherosclerosis in ApoE-/- mice suffering from high-fat diet and HSP65 aggression[J].Evid Based Complement Alternat Med,2019,2019:2531979.
    [9]
    Lu H,Daugherty A.Atherosclerosis[J].Arterioscler Thromb Vasc Biol,2015,35(3):485-491.
    [10]
    Netto Candido TL,Bressan J,Alfenas RCG.Dysbiosis and metabolic endotoxemia induced by high-fat diet[J].Nutr Hosp,2018,35(6):1432-1440.
    [11]
    Nasonov EL, Popkova TV. Atherosclerosis:perspectives of anti-inflammatory therapy[J].Ter Arkh,2018,90(5):4-12.
    [12]
    Poltorak A,He X,Smirnova I,et al.Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice:mutations in Tlr4 gene[J].Science,1998,282(5396):2085-2088.
    [13]
    Kayagaki N,Wong MT,Stowe IB,et al.Noncanonical inflammasome activation by intracellular LPS independent of TLR4[J].Science,2013,341(6151):1246-1249.
    [14]
    Rosadini CV,Kagan JC.Early innate immune responses to bacterial LPS[J].Curr Opin Immunol,2017,44:14-19.
    [15]
    Brinkhoff A,Sieberichs A,Engler H,et al.Pro-inflammatory Th1 and Th17 cells are suppressed during human experimental endotoxemia whereas anti-inflammatory IL-10 producing T-cells are unaffected[J].Front Immunol,2018,9:1133.
    [16]
    Ding FX, Fu Z, Liu B. Lipopolysaccharide exposure alleviates asthma in mice by regulating Th1/Th2 and treg/Th17 balance[J].Med Sci Monit,2018,24:3220-3229.

Catalog

    Article views (290) PDF downloads (767) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return