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GUO Congying, YANG Songlin, WANG Jun, LIAO Weitao, MO Lingfeng, ZHOU Danshui, NI Weiju, ZENG Yu. Regulation mechanism of Compound Yihe Tea on improving insulin resistance in obesity mice[J]. Journal of China Pharmaceutical University, 2020, 51(1): 68-75. DOI: 10.11665/j.issn.1000-5048.20200111
Citation: GUO Congying, YANG Songlin, WANG Jun, LIAO Weitao, MO Lingfeng, ZHOU Danshui, NI Weiju, ZENG Yu. Regulation mechanism of Compound Yihe Tea on improving insulin resistance in obesity mice[J]. Journal of China Pharmaceutical University, 2020, 51(1): 68-75. DOI: 10.11665/j.issn.1000-5048.20200111

Regulation mechanism of Compound Yihe Tea on improving insulin resistance in obesity mice

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  • The aim of this study was to investigate the effect of Compound Yihe Tea on improving insulin resistance in obesity mice. Thirty-two male C57BL/6J mice were randomly divided into 4 groups: the normal fat diet group(NFD group), high fat diet group(HFD group), Compound Yihe Tea low dosage group[20 mg/(kg ·d), YH-L group] and high dosage group[40 mg/(kg ·d), YH-H group]. NFD group was given standard feed, and the remaining mice were administered with high fat diet. After 6 weeks, YH-H and YH-L groups were given Compound Yihe Tea for 6 weeks. Blood glucose was measured at week 11 and serum levels of total cholesterol(TC), serum triglyceride(TG), low-density lipoprotein cholesterol(LDL-C)and high-density lipoprotein cholesterol(HDL-C)were measured at week 12. Liver tissues were prepared for oil red O and HE staining. Immunohistochemical analysis was used to test the protein expression of GLUT4 in liver. Protein expressions of PI3K, Akt and GLUT4 in epididymis white adipose tissue(WAT)were tested by Western blot. The results showed that Compound Yihe Tea could effectively reduce body weights and the serum levels of TC, TG and LDL-C. Furthermore Compound Yihe Tea could improve the histopathological changes of liver, up-regulate the protein expression of PI3K, Akt and GLUT4 in epididymis WAT and the protein expression of GLUT4 in liver. Compound Yihe Tea can reduce the fat accumulation in liver tissue, improve the indexes of blood glucose and lipid levels, and improve insulin resistance via PI3K-AKT-GLUT4 pathway.
  • [1]
    Shoelson SE,Herrero L,Naaz A.Obesity,inflammation,and insulin resistance[J].Gastroenterology,2007,132(6):2169-2180.
    [2]
    Samuel VT, Petersen KF, Shulman GI. Lipid-induced insulin resistance:unravelling the mechanism[J].Lancet,2010,375(9733):2267-2277.
    [3]
    Marengo A,Rosso C,Bugianesi E.Liver cancer:connections with obesity,fatty liver,and cirrhosis[J].Annu Rev Med,2016,67:103-117.
    [4]
    Madsen CM,Nordestgaard BG.Is it time for new thinking about high-density lipoprotein[J]?Arterioscler Thromb Vasc Biol,2018,38(3):484-486.
    [5]
    Leavens KF, Birnbaum MJ. Insulin signaling to hepatic lipid metabolism in health and disease[J].Crit Rev Biochem Mol Biol,2011,46(3):200-215.
    [6]
    Savage DB,Petersen KF,Shulman GI.Disordered lipid metabolism and the pathogenesis of insulin resistance[J].Physiol Rev,2007,87(2):507-520.
    [7]
    Li YR, Cheng T, Wang YS, et al. Effect of extract of selenium-enriched Astragalus membranaceus on insulin resistance in streptozotocin-induced diabetic rats[J].J China Pharm Univ(中国药科大学学报),2018,49(6):739-745.
    [8]
    Zimmermann R,Strauss JG,Haemmerle G,et al.Fat mobilization in adipose tissue is promoted by adipose triglyceride lipase[J].Science,2004,306(5700):1383-1386.
    [9]
    Garabadu D,Krishnamurthy S.Metformin attenuates hepatic insulin resistance in type-2 diabetic rats through PI3K/Akt/GLUT4 signalling independent to bicuculline-sensitive GABAA receptor stimulation[J].Pharm Biol,2017,55(1):722-728.
    [10]
    Xu H,Zhou Y,Liu YX,et al.Metformin improves hepatic IRS2/PI3K/Akt signaling in insulin-resistant rats of NASH and cirrhosis[J].J Endocrinol,2016,229(2):133-144.
    [11]
    Nie XQ, Chen HH, Zhang JY, et al. Rutaecarpine ameliorates hyperlipidemia and hyperglycemia in fat-fed,streptozotocin-treated rats via regulating the IRS-1/PI3K/Akt and AMPK/ACC2 signaling pathways[J].Acta Pharmacol Sin,2016,37(4):483-496.
    [12]
    Huang SH,Czech MP.The GLUT4 glucose transporter[J].Cell Metab,2007,5(4):237-252.
    [13]
    Watson RT,Kanzaki M,Pessin JE.Regulated membrane trafficking of the insulin-responsive glucose transporter 4 in adipocytes[J].Endocr Rev,2004,25(2):177-204.
    [14]
    Czech MP. Insulin action and resistance in obesity and type 2 diabetes[J].Nat Med,2017,23(7):804-814.
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