• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
TANG Gang, JIN Jian, YANG Ziyi. Determination of hydroxylsafflower yellow A in safflower W/O cream by RP-HPLC[J]. Journal of China Pharmaceutical University, 2020, 51(2): 161-167. DOI: 10.11665/j.issn.1000-5048.20200205
Citation: TANG Gang, JIN Jian, YANG Ziyi. Determination of hydroxylsafflower yellow A in safflower W/O cream by RP-HPLC[J]. Journal of China Pharmaceutical University, 2020, 51(2): 161-167. DOI: 10.11665/j.issn.1000-5048.20200205

Determination of hydroxylsafflower yellow A in safflower W/O cream by RP-HPLC

More Information
  • A reversed phase HPLC method for determination of hydroxylsafflower yellow A in safflower W/O cream was established. The column was Zorbax Eclipse C18 column(4. 6 mm×250 mm, 5 μm), and the mobile phase was composed of methanol, acetonitrile and 0. 02% phosphoric acid solution(26 ∶2〓 ∶72). The flow rate of mobile phase was set at 1. 0 mL/min, and the column temperature was kept at 55 °C. The detection wavelength was 403 nm. Safflower W/O cream was successively demulsified with methanol at high temperature and followed by the addition of purified water for the extraction. The results showed that the excipients did not interfere with the chromatographic peak of hydroxylsafflower yellow A. Hydroxylsafflower yellow A presented a good linear relationship in the range of 1. 236- 12. 36 μg/mL(y=156. 17x+1. 198 3, r=0. 999 5), and the detection limit was 23. 6 ng/mL with the quantitative limit of 118 ng/mL. The percentage of extracting recovery was in the range of 99. 7% to 103. 3%. The precision RSD was 0. 12%(n=6), and the sample stability was acceptable when being stored at room temperature for 24 h. The developed method in this study was simple, rapid, accurate and reproducible, and can be used for the determination of hydroxylsafflower yellow A in safflower W/O cream.
  • [1]
    Jin Y,Pu ZJ,Tang YP,et al.Effect of promoting blood circulation of herb pair containing angelicae sinensis radix and Carthami flos[J].Chin Tradit Herbal Drugs(中草药),2017,48(10):2087-2092.
    [2]
    Wang Y,Shi YJ,Zou JB,et al.Preparation and membrane permeability of phospholipid complex from extract of Carthamus tinctorius[J].Chin Tradit Herbal Drugs(中草药),2019,50(17):4084-4090.
    [3]
    Li C,Chang K.Clinical research progress of prevention and treatment of arnebia euchroma preparation on diaper dermatitis[J].World Chin Med(世界中医药),2017,12(8):1733-1738.
    [4]
    Kong DZ,Xia W,Zhang Z,et al.Safflower yellow injection combined with conventional therapy in treating unstable angina pectoris:a meta-analysis[J].J Tradit Chin Med(中医杂志),2013,33(5):553-561.
    [5]
    Nie YJZ,Luo Y,Li XY,et al.Extraction of active components from Carthamus tinctorius and evaluation of its effect on improving skin microcirculation[J].Chin Surfactant Detergent Cosmetics(日用化学工业),2019,49(5):304-309.
    [6]
    Xu HX,Wu YY,Pei J,et al.Correlation between flavonoids and color values of Carthamus tinctorius flower[J].J Chin Med Mater(中药材),2018,41(1):49-54.
    [7]
    Kang XL,Gu J,Tan R.Content determination of hydroxysaffor yellow A in Tibetan medicine Ershiwei Roudoukou Pills[J].China Pharm(中国药业),2015,24(19):39-40.
    [8]
    Yuan XM,Qing S,Jin X.Content determination of hydroxy safflower yellow pigment A in Mongolian medical children shikou powder[J].J Med Pharm Chin Minorities(中国民族医药杂志),2018,24(2):27-29.
    [9]
    Tang HQ,Zhang ZQ,Wang WQ,et al.Content determination of hydroxysafflor yellow A in Xuanbi lotion[J].Chin Med Heral(中国医药导报),2015,12(12):129-132.
    [10]
    Zhen DQ,Su YX,Wang YD.Study on the method of quantitative determination of hydroxysafflor yellow A in safflor yellow eye drops[J].Pharm Today(今日药学),2016,26(10):687-690.
    [11]
    Wang J,Sun Y,Li WC.Improving quality standards of ccompound lincomycin cream[J].Tianjin Pharm(天津药学),2018,30(3):17-19.
    [12]
    Guo CZ,Wang DK,Hu YY.Determination of related substances and content of icotinib hydrochloride cream by HPLC[J].Chin J New Drugs(中国新药杂志),2015,24(21):2516-2520.
    [13]
    Ahmad A,Adewunmi,Muhammad SK.Demulsification of water-in-oil emulsions using ionic liquids:effects of counterion and water type[J].J Mol Liq,2019,279:411-419.
    [14]
    Sebastian H,angar S.Ahmed1,et al.Emulsification of water/crude oil emulsion using natural rock alginite[J].Colloids Surf A,2018,553:71-79.
    [15]
    Wang SC.HPLC determination of saffcomin A in Sanshiwuwei Chenxiang pills[J].J China Pharm Univ(中国药科大学学报),2008,39(6):582-583.
    [16]
    Zhang HF,Guo XJ,Huang LS.Absorption mechanism of hydroxysafflor yellow A in rats[J].J China Pharm Univ(中国药科大学学报),2006,37(4):312-317.
  • Cited by

    Periodical cited type(1)

    1. 毕秋左,安艳苏. HPLC法同时测定中消软膏中4种成分含量. 中国民族民间医药. 2023(16): 48-51 .

    Other cited types(1)

Catalog

    Article views (574) PDF downloads (850) Cited by(2)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return