Effect of celastrol combined medication on the inhibition of HepG2 cell proliferation

ZHANG Xue; OUYANG Huizhi; XU Wei; WANG Xiaoying

doi:10.11665/j.issn.1000-5048.20200209

Journal of China Pharmaceutical University > 2020 > 51(2): 185-192. > DOI: 10.11665/j.issn.1000-5048.20200209

ZHANG Xue, OUYANG Huizhi, XU Wei, WANG Xiaoying. Effect of celastrol combined medication on the inhibition of HepG2 cell proliferation[J]. Journal of China Pharmaceutical University, 2020, 51(2): 185-192. DOI: 10.11665/j.issn.1000-5048.20200209

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Effect of celastrol combined medication on the inhibition of HepG2 cell proliferation

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Abstract

To study the inhibitory effect of celastrol respectively combined with glycyrrhetic acid, paclitaxel, and rhein on the proliferation of human hepatoma cell line HepG2. The MTT method was used to detect the survival rate of HepG2 cells. The cooperativity index(CI)and Jin′s formula method were used to determine the synergistic effect. Apoptosis and cell cycle arrest were detected, too. The results show that celastrol, glycyrrhetinic acid, rhein, and paclitaxel alone can inhibit the proliferation of HepG2 cells, respectively. Combination with glycyrrhetic acid, paclitaxel, and rhein, respectively, the inhibitory effect of celastrol on the proliferation of HepG2 cells was significantly enhanced. And the synergistic effect on the proliferation inhibition of HepG2 cells in some concentrations was displayed in the experiment. The cell apoptosis rate was improved(P< 0. 01)and more cells were arrested in G₂/M phase. Celastrol respectively combined with glycyrrhetic acid, paclitaxel, and rhein displayed a synergistic inhibitory effect on the proliferation of HepG2 cells, and the effect was related to inducing cell apoptosis and increasing the cell cycle arrest in G₂/M phase.
- celastrol,
- rhein,
- paclitaxel,
- glycyrrhetic acid,
- HepG2 cells,
- combined medication,
- synergy effect

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