Effects of cerebroprotein hydrolysate for injection (II) on neuritogenesis and its underlying mechanisms

WEI Dasha; GUAN Xin; ZHANG Shengbin; YU Fang; WANG Cunfang; ZHOU Yu; PANG Tao

doi:10.11665/j.issn.1000-5048.20210211

Journal of China Pharmaceutical University > 2021 > 52(2): 219-226. > DOI: 10.11665/j.issn.1000-5048.20210211

WEI Dasha, GUAN Xin, ZHANG Shengbin, YU Fang, WANG Cunfang, ZHOU Yu, PANG Tao. Effects of cerebroprotein hydrolysate for injection (II) on neuritogenesis and its underlying mechanisms[J]. Journal of China Pharmaceutical University, 2021, 52(2): 219-226. DOI: 10.11665/j.issn.1000-5048.20210211

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Effects of cerebroprotein hydrolysate for injection (II) on neuritogenesis and its underlying mechanisms

1.
Center for New Drug Screening, Institute of Pharmaceutical Sciences, China Pharmaceutical University, Nanjing 210009
2.
Guangdong Longfu Pharmaceutical Co., Ltd., Zhongshan 528451, China

Funds: This study was supported by the National Natural Science Foundation of China (No.81973512)

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Received Date: September 10, 2020
Revised Date: March 04, 2021

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Abstract

Abstract

In most mammalian central nervous system diseases, axons are damaged.Due to the limited ability of damaged neurons to promote axonal regeneration, the formation of glial scar and the release of inhibitory nutrients, it is difficult to regenerate axons of damaged neurons. The purpose of this study was to investigate the effect of cerebroprotein hydrolysate for injection (II) (CBL) on neuritogenesis and its underlying mechanism. Immunofluorescence staining was used to detect the axon length of mouse neuroma cells (Neuro-2a) and mouse primary cortical neuronal cells. Western blotting was used to detect the expression of phosphorylated TrkB protein in Neuro-2a cells and mouse primary cortical neuronal cells. The results showed that CBL could increase the axon length of Neuro-2a cells or mouse primary cortical neuronal cells, and that the phosphorylation level of TrkB in neuronal cells was significantly increased when 5 μg/mL CBL was applied to neuronal cells for 1 h. In conclusion, CBL can promote neuritogenesis, and increase the expression of phosphorylated TrkB, which may be related to the activation of TrkB signaling pathway.
- cerebroprotein hydrolysate for injection (II),
- neuritogenesis,
- TrkB,
- neurological disorders

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References

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