Effects and mechanisms of microRNA-23b on neuronal apoptosis induced by Aβ<sub>25-35</sub>

PAN Kemeng; CHEN Song; GAO Xiangdong; YAO Wenbing

doi:10.11665/j.issn.1000-5048.20210411

Journal of China Pharmaceutical University > 2021 > 52(4): 480-486. > DOI: 10.11665/j.issn.1000-5048.20210411

PAN Kemeng, CHEN Song, GAO Xiangdong, YAO Wenbing. Effects and mechanisms of microRNA-23b on neuronal apoptosis induced by Aβ_25-35[J]. Journal of China Pharmaceutical University, 2021, 52(4): 480-486. DOI: 10.11665/j.issn.1000-5048.20210411

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Effects and mechanisms of microRNA-23b on neuronal apoptosis induced by Aβ_25-35

Jiangsu Key Laboratory of Druggability of Biopharmaceuticals, School of Life Science and Technology, China Pharmaceutical University, Nanjing 211198, China

Funds: This study was supported by the National Natural Science Foundation of China (No.81872850, No.81673435) and the "Double First-Class" Project of China Pharmaceutical University (No.CPU2018GF08)

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Received Date: December 27, 2020
Revised Date: April 26, 2021

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Abstract

Abstract

In this study, the effects and mechanisms of miR-23b in the AD cell model were explored. Aβ_25-35 was used to induce neuronal injury model, and cell viabilities were detected by MTT assay. The effect of miR-23b on the apoptotic levels of Aβ_25-35-induced SH-SY5Y cells was analyzed using Annexin V-FITC/PI detection kit. The effect of miR-23b on the mitochondrial membrane potential of Aβ_25-35-induced SH-SY5Y cells was examined using JC-1 fluorescent probe. The levels of cell apoptosis-related proteins and autophagy-related proteins were detected by Western blot. The results showed that miR-23b could alleviate the apoptosis and the abnormal mitochondrial membrane potential in SH-SY5Y cells induced by Aβ_25-35.This study suggested that miR-23b may attenuate Aβ_25-35-induced neuronal apoptosis by regulating apoptosis and autophagy-related pathway.
- microRNA-23b,
- Alzheimer′s disease,
- β-amyloid,
- apoptosis,
- autophagy

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References

[1]	. Cell Physiol Biochem，2014，34（1）：92-110.
[2]	Duyckaerts C，Delatour B，Potier MC. Classification and basic pathology of Alzheimer disease［J］. Acta Neuropathol，2009，118（1）：5-36.
[3]	Fessel J. Alzheimer′s disease combination treatment［J］. Neurobiol Aging，2018，63：165.
[4]	Alzheimer′s Association. 2020 Alzheimer′s disease facts and figures［J］. Alzheimers Dement，2020，16：391-460.
[5]	Filipowicz W，Bhattacharyya SN，Sonenberg N. Mechanisms of post-transcriptional regulation by microRNAs：are the answers in sight［J］. Nat Rev Genet，2008，9：102-114.
[6]	Swarbrick S，Wragg N，Ghosh S，et al. Systematic review of miRNA as biomarkers in Alzheimer′s disease［J］. Mol Neurobiol，2019，56：6156-6167.
[7]	Wang X，Liu D，Huang HZ，et al. A novel microRNA-124/PTPN1 signal pathway mediates synaptic and memory deficits in Alzheimer′s disease［J］. Biol Psychiatry，2018，83：395-405.
[8]	Qureshi IA，Mehler MF. Emerging roles of non-coding RNAs in brain evolution，development，plasticity and disease［J］. Nat Rev Neurosci，2012，13（8）：528-541.
[9]	Wang W，Wang Y，Liu W，et al. Regulation and biological roles of the multifaceted miRNA-23b （MIR23B）［J］. Gene，2018，642：103-109.
[10]	Hu H，Tang J，Liu C，et al. MiR-23b promotes the migration of keratinocytes through downregulating TIMP3［J］. J Surg Res，2020，254：102-109.
[11]	Han H，Zhang Z，Yang X，et al. MiR-23b suppresses lung carcinoma cell proliferation through CCNG1［J］. Oncol Lett，2018，16（4）：4317-4324.
[12]	Gasiul? S，Dreize N，Kaupinis A，et al. Molecular insights into miRNA-driven resistance to 5-fluorouracil and oxaliplatin chemotherapy：miR-23b modulates the epithelial-mesenchymal transition of colorectal cancer cells［J］. J Clin Med，2019，8（12）：2115.
[13]	Weinberg RB，Mufson EJ，Counts SE. Evidence for a neuroprotective microRNA pathway in amnestic mild cognitive impairment［J］. Front Neurosci，2015，9：430.
[14]	Lugli G，Cohen AM，Bennett DA，et al. Plasma exosomal miRNAs in persons with and without Alzheimer disease：altered expression and prospects for biomarkers［J］. PLoS One，2015，10（10）：e0139233.
[15]	Chen YJ，Gao XD，Chen S. Effects and mechanisms of FGF21 on neuronal damage induced by rotenone［J］. J China Pharm Univ （中国药科大学学报），2020，51（6）：718-723.
[16]	Ji Y，Shi Z，Zhang Y，et al. Prevalence of dementia and main subtypes in rural northern China［J］. Dement Geriatr Cogn Disord，2015，39（5/6）：294-302.
[17]	Revi M. Alzheimer′s disease therapeutic approaches［J］. Adv Exp Med Biol，2020，1195：105-116.
[18]	Fabian MR，Sonenberg N. The mechanics of miRNA-mediated gene silencing：a look under the hood of miRISC［J］. Nat Struct Mol Biol，2012，19：586-593.
[19]	Liu H，Wei W，Wang X，et al. MiR23b3p promotes the apoptosis and inhibits the proliferation and invasion of osteosarcoma cells by targeting SIX1［J］. Mol Med Rep，2018，18（6）：5683-5692.
[20]	Hu X，Wang Y，Liang H，et al. MiR-23a/b promote tumor growth and suppress apoptosis by targeting PDCD4 in gastric cancer［J］. Cell Death Dis，2017，8（10）：e3059.
[21]	Sun L，Liu A，Zhang J，et al. MiR-23b improves cognitive impairments in traumatic brain injury by targeting ATG12-mediated neuronal autophagy［J］. Behav Brain Res，2018，340：126-136.
[22]	Selkoe DJ. American college of physicians，American physiological society. Alzheimer disease：mechanistic understanding predicts novel therapies［J］. Ann Intern Med，2004，140（8）：627-638.
[23]	Selkoe DJ，Hardy J. The amyloid hypothesis of Alzheimer''s disease at 25 years［J］. EMBO Mol Med，2016，8（6）：595-608.
[24]	De Felice FG，Velasco PT，Lambert MP，et al. A beta oligomers induce neuronal oxidative stress through an N-methyl-D-aspartate receptor-dependent mechanism that is blocked by the Alzheimer drug memantine［J］. J Biol Chem，2007，282（15）：11590-11601.
[25]	Millucci L，Raggiaschi R，Franceschini D，et al. Rapid aggregation and assembly in aqueous solution of A beta （25-35） peptide［J］. J Biosci，2009，34（2）：293-303.
[26]	Sun Y，Gao XD，Chen S. Effect and mechanism of FGF21 on astrocyte damage induced by Aβ_25-35［J］. J China Pharm Univ （中国药科大学学报），2019，50（4）：490-496.
[27]	de Medeiros LM，De Bastiani MA，Rico EP，et al. Cholinergic differentiation of human neuroblastoma SH-SY5Y cell line and its potential use as an in vitro model for Alzheimer''s disease Studies［J］. Mol Neurobiol，2019，56（11）：7355-7367.
[28]	Wan D，Wu Q，Qu W，et al. Pyrrolidine dithiocarbamate （PDTC） inhibits DON-induced mitochondrial dysfunction and apoptosis via the NF-κB/iNOS pathway［J］. Oxid Med Cell Longev，2018，2018：1324173.
[29]	Lu T，Aron L，Zullo J，et al. REST and stress resistance in ageing and Alzheimer′s disease［J］. Nature，2014，507（7493）：448-454.
[30]	Lv XH，Zhao DH，Cai SZ，et al. Autophagy plays a protective role in cell death of osteoblasts exposure to lead chloride［J］. Toxicol Lett，2015，239（2）：131-140.
[31]	Ghavami S，Shojaei S，Yeganeh B，et al. Autophagy and apoptosis dysfunction in neurodegenerative disorders［J］. Prog Neurobiol，2014，112：24-49.
[32]	Liu J，Liu W，Lu Y，et al. Piperlongumine restores the balance of autophagy and apoptosis by increasing BCL2 phosphorylation in rotenone-induced Parkinson disease models［J］. Autophagy，2018，14（5）：845-861.

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