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WANG Lixin, JIANG Xiubo, GUO Qiaozhen, WANG Zicheng, WANG Bo, WANG Yuxia, QU Wensheng, DUAN Xiaotao. TM9SF2 promotes proliferation and metastasis of triple negative breast cancer cell line MDA-MB-231[J]. Journal of China Pharmaceutical University, 2021, 52(5): 609-613. DOI: 10.11665/j.issn.1000-5048.20210514
Citation: WANG Lixin, JIANG Xiubo, GUO Qiaozhen, WANG Zicheng, WANG Bo, WANG Yuxia, QU Wensheng, DUAN Xiaotao. TM9SF2 promotes proliferation and metastasis of triple negative breast cancer cell line MDA-MB-231[J]. Journal of China Pharmaceutical University, 2021, 52(5): 609-613. DOI: 10.11665/j.issn.1000-5048.20210514
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TM9SF2 promotes proliferation and metastasis of triple negative breast cancer cell line MDA-MB-231

  • 1.

    School of Chemical Engineering,Tianjin University,Tianjin 300350

  • 2.

    State Key Laboratory of Antitoxic Drugs and Toxicology,Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850

  • 3.

    College of Biological and Pharmaceutical Engineering, Nanjing Tech University,Nanjing, 211800, China

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  • Received Date: February 22, 2021
  • Revised Date: May 09, 2021
    Graphical Abstract
    • Abstract
  • The aim of this study was to investigate the effect of transmembrane 9 superfamily protein member 2 (TM9SF2) in proliferation and migration of triple negative breast cancer cell line MDA-MB-231.The expression of TM9SF2 in triple negative breast cancer cell line MDA-MB-231 and nontumorigenic mammary epithelial cell line MCF-10A were measured by Western blot. MDA-MB-231 cells were treated with siRNA-TM9SF2 to knock-down the expression of TM9SF2. The effect of silencing TM9SF2 was measured with Western blot.The proliferation of cells was tested by MTS,and the migration was measured with Transwell and wound-healing assay.Proteins related to proliferation (PI3K,AKT,SRC and ERK) and migration (Snail,Slug and N-cadherin) were measured with Western blot.Protein expressions of TM9SF2 was better improved in triple negative breast cancer MDA-MB-231 cell line than MCF-10A.Compared with the control group, the siRNA-TM9SF2 infected group had lower expressions of PI3K, Snail, Slug and N-cadherin, and at the same time phosphorylation of AKT was decreased. The results suggest TM9SF2 can promote the proliferation and metastasis of triple negative breast cancer MDA-MB-231 cell line.
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    • References (16)
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