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XIA Ying, LONG Jiayi, DAI Haoran, SHEN Mengyuan, GUO Hongli, HU Yahui, CHEN Feng. Determination of digoxin in human plasma by LC-MS/MS and its application in pediatric patients[J]. Journal of China Pharmaceutical University, 2021, 52(6): 719-724. DOI: 10.11665/j.issn.1000-5048.20210610
Citation: XIA Ying, LONG Jiayi, DAI Haoran, SHEN Mengyuan, GUO Hongli, HU Yahui, CHEN Feng. Determination of digoxin in human plasma by LC-MS/MS and its application in pediatric patients[J]. Journal of China Pharmaceutical University, 2021, 52(6): 719-724. DOI: 10.11665/j.issn.1000-5048.20210610
shu

Determination of digoxin in human plasma by LC-MS/MS and its application in pediatric patients

  • 1.

    Pharmaceutical Sciences Research Center, Department of Pharmacy, Children's Hospital of Nanjing Medical University, Nanjing 210008

  • 2.

    School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing 210009

  • 3.

    Kangda College of Nanjing Medical University, Lianyungang 222000 , China

Funds: This study was supported by Specially-Appointed Medical Expert Project of Jiangsu Provincial Commission of Health (2019)
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  • Received Date: August 23, 2021
  • Revised Date: November 03, 2021
    Graphical Abstract
    • Abstract
  • The aim of the study was to develop a simple, rapid and accurate LC-MS/MS method for the determination of digoxin.Digoxin-d3 was taken as the internal standard (IS), and sample preparation was achieved by liquid-liquid extraction.Chromatographic separation was performed on a Kinetex C18 column (2.1 mm × 50 mm, 2.6 μm; Phenomenex) using an isocratic elution with merely 2 min for each sample.The mobile phase consisted of water and acetonitrile solutions, both containing 1 mmol/L ammonium acetate and 1 mmol/L formic acid (55∶45).The detection was conducted on a TripleQuadTM 4500MD mass spectrometer coupled with electrospray ionization interface under positive-ion multiple reaction monitoring mode.The transitions were m/z 798.5 → 651.3 and m/z 801.6 → 654.4 for digoxin and digoxin-d3, respectively.Results showed that the method was linear over the range of 0.100-20.0 ng/mL.The selectivity, accuracy and precision, recovery and stability of the method were all within the acceptable limits with no matrix effect.This method was successfully applied to a girl treated with digoxin with substantial improvement of therapeutic effect and elimination of toxic reaction, so it can provide valuable fuidance and reference for individualized medication in clinical practice.
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    • References (14)
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