Effect and mechanism of muscle injury on liver lipid metabolism in Duchenne muscular dystrophy mice

ZHANG Ziling; LU Qian; XU Dengqiu; YU Qinwei; JIANG Zhenzhou

doi:10.11665/j.issn.1000-5048.20210612

Journal of China Pharmaceutical University > 2021 > 52(6): 735-741. > DOI: 10.11665/j.issn.1000-5048.20210612

ZHANG Ziling, LU Qian, XU Dengqiu, YU Qinwei, JIANG Zhenzhou. Effect and mechanism of muscle injury on liver lipid metabolism in Duchenne muscular dystrophy mice[J]. Journal of China Pharmaceutical University, 2021, 52(6): 735-741. DOI: 10.11665/j.issn.1000-5048.20210612

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Effect and mechanism of muscle injury on liver lipid metabolism in Duchenne muscular dystrophy mice

1.
Jiangsu Key Laboratory of Drug Screening, China Pharmaceutical University, Nanjing 210009
2.
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009
3.
Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, China Pharmaceutical University, Nanjing 210009, China

Funds: This study was supported by the National Natural Science Foundation of China (No.81773827), the Scholar of the 14th batch of "Six Talents Peak" High-Level Talent Selection (No.SWYY-094), the "Double First-Class" University Project (No.CPU2018GY33), and the Postgraduate Research Practice Innovation Program of Jiangsu Province (No.KYCX19_0763)

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Received Date: May 17, 2021
Revised Date: October 27, 2021

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Abstract

Abstract

Duchene muscular dystrophy (DMD) is a serious progressive muscular dystrophy.Reports in recent years about abnormal lipid in DMD patients have increased, yet little attention has been paid to liver lipid.This study aimed to explore the effect of dystrophin gene defect on liver lipid synthesis.7-week-old mdx male mice were used as DMD model.The conditions of liver function, liver lipid accumulation and liver lipid synthesis were determined through liver tissue morphological examination, blood biochemical examination, and detection of hepatic gene and protein expression.The results showed that lipid droplets in liver of mdx mice increased significantly.The contents of total cholesterol and triglyceride in liver, aspartate aminotransferase and alanine aminotransferase in serum increased.The gene and protein expression of hepatic lipid synthesis-related enzymes such as fatty acid synthase, acetyl CoA carboxylase, and sterol regulatory element binding protein 1-c were up-regulated.These results showed accumulation of liver lipid in 7-week-old mdx male mice.
- Duchene muscular dystrophy,
- skeletal muscle injury,
- liver lipid accumulation,
- lipid synthesis

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References (21)

References

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