Determination of dezocine and pethidine in human hair by UPLC-MS/MS

SHI Xiaolu; QIAO Hongwei; WU Jianmei; DI Bin; WANG Youmei

doi:10.11665/j.issn.1000-5048.20220111

Journal of China Pharmaceutical University > 2022 > 53(1): 74-78. > DOI: 10.11665/j.issn.1000-5048.20220111

SHI Xiaolu, QIAO Hongwei, WU Jianmei, DI Bin, WANG Youmei. Determination of dezocine and pethidine in human hair by UPLC-MS/MS[J]. Journal of China Pharmaceutical University, 2022, 53(1): 74-78. DOI: 10.11665/j.issn.1000-5048.20220111

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Determination of dezocine and pethidine in human hair by UPLC-MS/MS

1.
School of Pharmacy, China Pharmaceutical University, Nanjing 210009
2.
Key Laboratory of Drug Monitoring and Control, Beijing 100741
3.
Drug Intelligence and Forensic Center, Ministry of Public Security of the People Republic of China, Beijing 100741, China

Funds: This study was supported by the Special Project of Basic Work of Strengthening Police by Science and Technology of Ministry of Public Security (No. 2020GABJC22)

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Received Date: April 09, 2021
Revised Date: November 28, 2021

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Abstract

Abstract

A rapid analytical method for the determination of dezocine and pethidine in hair samples using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established.After cleaned hair was extracted by grinding with methanol and ultrasonic, the final solution was analyzed by UPLC-MS/MS.The targets were gradient eluted on a Waters Acquity BEH C₁₈ (2.1 mm × 100 mm, 1.7 μm) column with 0.1% formic acid-water and methanol as mobile phase at a flow rate of 0.4 mL/min.The ESI⁺ion source and multiple reaction monitoring (MRM) were used to select the qualitative and quantitative ion pairs of dezocine and pethidine.Dezocine and pethidine showed good linearity in the range of 0.01-8 ng/mg, with the limit of detection of 0.005 ng/mg and the LOQs of 0.01 ng/mg.The accuracy, precision, matrix effect, extraction recovery, and stability all met the requirements.The established method is simple, rapid, and accurate for the qualitative and quantitative determination of dezocine and pethidine in hair, which can be applied in the case analysis of dezocine and/or pethidine abuse.
- dezocine,
- pethidine,
- hair analysis,
- UPLC-MS/MS

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