Synthesis and protective effect of carboxymethylcysteine L-arginate in bronchial epithelial cells

JIA Jian; WU Jianbing; ZHANG Yihua; HUANG Zhangjian

doi:10.11665/j.issn.1000-5048.20220206

Journal of China Pharmaceutical University > 2022 > 53(2): 171-177. > DOI: 10.11665/j.issn.1000-5048.20220206

JIA Jian, WU Jianbing, ZHANG Yihua, HUANG Zhangjian. Synthesis and protective effect of carboxymethylcysteine L-arginate in bronchial epithelial cells[J]. Journal of China Pharmaceutical University, 2022, 53(2): 171-177. DOI: 10.11665/j.issn.1000-5048.20220206

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Synthesis and protective effect of carboxymethylcysteine L-arginate in bronchial epithelial cells

Center of Drug Discovery, China Pharmaceutical University, Nanjing 210009, China

Funds: This study was supported by the National Natural Science Foundation of China (No.81822041, No.82173681, No.21977116,No.82104004, No.81773573)

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Received Date: December 18, 2021
Revised Date: March 14, 2022

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Abstract

Abstract

Carboxymethylcysteine (CMC) is a common drug for the clinical treatment of chronic obstructive pulmonary disease, yet its long-term use can cause severe irritation to the gastrointestinal tract.As the substrate of nitric oxide (NO) synthase (NOS), L-arginine can be converted in the body into NO beneficial to the cardiovascular system, the gastrointestinal tract and so on.As a basic amino acid, L-arginine can be salified with some compounds containing acidic groups to improve the water solubility of the parent drug and may enhance the activity and alleviate side effects due to NO release.In this study, we designed and synthesized carboxymethylcysteine L-arginate (CMCA), and tested its physico-chemical properties, and the abilities to scavenge reactive oxygen species (ROS), inhibit apoptosis and release NO in cigarette smoke-induced injury model of human bronchial epithelial cells.The results revealed that CMCA is superior to CMC or L-arginine in that it could capture ROS, release NO and suppress apoptosis, suggesting that CMCA is worthy of further research and development.
- chronic obstructive pulmonary disease,
- carboxymethylcysteine,
- nitric oxide,
- L-arginine,
- reactive oxygen species,
- apoptosis

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