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QIAN Jinzhu, LIANG Ming, YU Yi, DENG Yalei, LIU Shijia, PANG Huiming, QI Jin. Determination of antioxidant activity of Polygoni Multiflori Radix Praeparata by HPLC-DPPH[J]. Journal of China Pharmaceutical University, 2022, 53(2): 185-191. DOI: 10.11665/j.issn.1000-5048.20220208
Citation: QIAN Jinzhu, LIANG Ming, YU Yi, DENG Yalei, LIU Shijia, PANG Huiming, QI Jin. Determination of antioxidant activity of Polygoni Multiflori Radix Praeparata by HPLC-DPPH[J]. Journal of China Pharmaceutical University, 2022, 53(2): 185-191. DOI: 10.11665/j.issn.1000-5048.20220208
shu

Determination of antioxidant activity of Polygoni Multiflori Radix Praeparata by HPLC-DPPH

  • 1.

    School of Traditional Chinese Medicine Pharmacy, China Pharmaceutical University, Nanjing 211198

  • 2.

    Infinitus (China) Co., Ltd.,Guangzhou 510405

  • 3.

    Department of Pharmacy, Jiangsu Provincial Hospital of Chinese Medicine, Nanjing 210029, China

Funds: This study was supported by Chinese Medicine Technology Development Project of Jiangsu Province (No.2020ZX11) and the Science and Technology Project of Jiangsu Administration of Traditional Chinese Medicine (No.JD2019SZ14)
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  • Received Date: October 10, 2021
  • Revised Date: March 03, 2022
    Graphical Abstract
    • Abstract
  • An on-line HPLC-DPPH system was developed to determine the antioxidant activity of 16 batches of Polygoni Multiflori Radix Praeparata. By analyzing the chromatographic and biological activity fingerprints of 16 batches of Polygoni Multiflori Radix Praeparata, the dose-effect relationship was established and the total antioxidant activity was quantified by activity addition.The results suggested that the online HPLC-DPPH method can evaluate the antioxidant activity of different bathches of Polygoni Multiflori Radix Praeparata, with different processing methods, aiming to provide datasupport and scientific basis forquality evaluation of Polygoni Multiflori Radix Praeparata.
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    • References (21)
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