Identification of chemical constituents in Fufang Shechuangzi Xiji（Lotion）by UPLC-Q-TOF-MS/MS

CHENG Xiaohong; JIANG Li; DONG Xingting; KANG Xiulin; LIU Lifang

doi:10.11665/j.issn.1000-5048.20220408

Journal of China Pharmaceutical University > 2022 > 53(4): 452-466. > DOI: 10.11665/j.issn.1000-5048.20220408

CHENG Xiaohong, JIANG Li, DONG Xingting, KANG Xiulin, LIU Lifang. Identification of chemical constituents in Fufang Shechuangzi Xiji（Lotion）by UPLC-Q-TOF-MS/MS[J]. Journal of China Pharmaceutical University, 2022, 53(4): 452-466. DOI: 10.11665/j.issn.1000-5048.20220408

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Identification of chemical constituents in Fufang Shechuangzi Xiji（Lotion）by UPLC-Q-TOF-MS/MS

1.
Hospital for Skin Diseases, Chinese Academy of Medical Sciences, Nanjing 210042
2.
Department of Pharmacognosy, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China

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Received Date: March 31, 2022
Revised Date: May 26, 2022

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Abstract

Abstract

Fufang Shechuangzi Xiji (Lotion) is a common hospital preparation made from clinical prescriptions. Its clinical efficacy is accurate, yet its pharmacodynamic material basis has not been clarified.An ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS) technique was developed to identify the chemical constituents of Fufang Shechuangzi Xiji (Lotion).The chemical constituents of Fufang Shechuangzi Xiji (Lotion) were scanned by electrospray ionization (ESI) source in positive and negative ion modes.As a result, a total of 118 compounds were identified and characterized via reference standards and by comparing mass spectrometry data with literature, including 45 alkaloids, 21 coumarins and chromones, 19 flavonoids, 14 saponins, 10 anthraquinones and 9 organic acids.As a result, UPLC-QTOF-MS/MS technology can quickly and sensitively identify the chemical constituents of Fufang Shechuangzi Xiji (Lotion), which provides a useful reference for exploring the pharmacodynamic material basis and further quality control study of the preparation.
- Fufang Shechuangzi Xiji（Lotion）,
- UPLC-Q-TOF-MS/MS,
- chemical constituents,
- effective substances

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Identification of chemical constituents in Fufang Shechuangzi Xiji（Lotion）by UPLC-Q-TOF-MS/MS

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