Application of zebrafish model in the metabolism of new psychoactive substances

XU Linhao; LIU Xinze; LIU Wei; XIANG Ping; HANG Taijun; YANG Shuo; YAN Hui

doi:10.11665/j.issn.1000-5048.20220511

Journal of China Pharmaceutical University > 2022 > 53(5): 606-612. > DOI: 10.11665/j.issn.1000-5048.20220511

XU Linhao, LIU Xinze, LIU Wei, XIANG Ping, HANG Taijun, YANG Shuo, YAN Hui. Application of zebrafish model in the metabolism of new psychoactive substances[J]. Journal of China Pharmaceutical University, 2022, 53(5): 606-612. DOI: 10.11665/j.issn.1000-5048.20220511

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Application of zebrafish model in the metabolism of new psychoactive substances

1.
Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Science Platform, Key Laboratory of Forensic Science, Ministry of Justice, Academy of Forensic Science, Shanghai 200063
2.
School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China

Funds: This study was supported by Shanghai Talent Development Project (No.2019095); the Natural Science Foundation of Shanghai (No.19ZR1458800); the Project of Shanghai Key Laboratory of Forensic Medicine (No.21DZ2270800); and the Project of Shanghai Judicial Expertise Professional and Technical Service Platform (No.19DZ2292700)

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Received Date: May 09, 2022
Revised Date: September 12, 2022

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Abstract

Abstract

Zebrafish is a novel model organism for drug metabolism research because of its small size, easy feeding, low reproduction cost and high reproduction rate.This paper outlines the construction of zebrafish metabolic model, illustrating the advantages of zebrafish model with high genetic similarity to human, well-developed enzyme system, and low substrate effect; and then reviews the application of zebrafish model in the synthesis of various new psychoactive substances such as cannabinoids, fentanyl, piperazines, and synthetic cathinones.The common metabolic reactions in zebrafish, including phase I (oxidation, N-demethylation, O-deethylation, hydroxylation and N-desalkylation) and phase II (sulfation and glucuronidation) reactions, are summarized, and the differences between the metabolites of zebrafish and those of real human body fluid samples are mainly related to the types of enzymes.This paper also points out the great potential and further research trends of zebrafish model in the study of new psychoactive substances.
- zebrafish model,
- new psychoactive substance,
- metabolite,
- application

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References (66)

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