Secondary metabolites of the endophytic fungus <i>Aspergillus</i> sp.Dq-25 from barnacle

CAI Caihong; ZHENG Hao; GAI Cuijuan; DAI Haofu; MEI Wenli; CHEN Huiqin

doi:10.11665/j.issn.1000-5048.20220531001

Journal of China Pharmaceutical University > 2023 > 54(1): 62-67. > DOI: 10.11665/j.issn.1000-5048.20220531001

CAI Caihong, ZHENG Hao, GAI Cuijuan, DAI Haofu, MEI Wenli, CHEN Huiqin. Secondary metabolites of the endophytic fungus Aspergillus sp.Dq-25 from barnacle[J]. Journal of China Pharmaceutical University, 2023, 54(1): 62-67. DOI: 10.11665/j.issn.1000-5048.20220531001

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Secondary metabolites of the endophytic fungus Aspergillus sp.Dq-25 from barnacle

1.
Hainan Provincial Key Laboratory for Research and Development of Natural Product from Li Folk Medicine, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101
2.
Hainan Institute for Tropical Agricultural Resources, Haikou 571101, China

Funds: This study was supported by the Natural Science Foundation of Hainan Province (No.220RC702), and Special Financial Project of Ministry of Agriculture and Rural Affairs (No.NFZX2021)

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Received Date: May 30, 2022
Revised Date: March 02, 2023

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Abstract

Abstract

The chemical constituents of solid rice culture of the endophytic fungus Aspergillus sp.Dq-25 from barnacle were isolated and purified by silica gel, Sephadex LH-20, C₁₈reversed silica gel column chromatography and recrystallization.Their structures were identified by the physical and chemical properties, and by various spectroscopic methods.Six compounds were isolated and identified as: demethyldihydropenicillic acid (1), dihydropenicillic acid (2), penicillic acid (3), fortisterol (4), 22E, 24R-3P, 5a-dihydroxyerogosta-7, 22-diene-6-one (5), and (22E, 24R)-ergosterol-7, 22-diene-3β, 5α, 9α-triol-6-one (6).Compound 1 was a new butyrolactone.MTT method was used to analyze cytotoxicity, and the result showed that compound 3 exhibited inhibitory activity on five cell lines, including K562, HeLa, SGC-7901, A542 and BEL-7402, with IC₅₀ values of 38.0 ~ 105.0 μmol/L.
- barnacle,
- endophytic fungus,
- Aspergillus sp.,
- γ-butyrolactone,
- chemical constituents,
- isolation and identification,
- cytotoxicity

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