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HUANG Zhihuan, LIANG Xiao, GAO Xiangdong, CHEN Song. Effects and mechanisms of SNP-9 on Parkinson''s disease cell model induced by rotenone[J]. Journal of China Pharmaceutical University, 2023, 54(2): 238-244. DOI: 10.11665/j.issn.1000-5048.2023020101
Citation: HUANG Zhihuan, LIANG Xiao, GAO Xiangdong, CHEN Song. Effects and mechanisms of SNP-9 on Parkinson''s disease cell model induced by rotenone[J]. Journal of China Pharmaceutical University, 2023, 54(2): 238-244. DOI: 10.11665/j.issn.1000-5048.2023020101
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Effects and mechanisms of SNP-9 on Parkinson''s disease cell model induced by rotenone

  • Jiangsu Provincial Key Laboratory of Druggability of Biopharmaceuticals, School of Life Science and Technology, China Pharmaceutical University, Nanjing 211198, China

Funds: This study was supported by the National Natural Science Foundation of China (No. 82073755, No. 82173728); and the National Innovation and Entrepreneurship Training Program for Undergraduate
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  • Received Date: January 31, 2023
  • Revised Date: March 20, 2023
    Graphical Abstract
    • Abstract
  • In this article, the effects and mechanisms of SNP-9 on Parkinson''s disease (PD) cell model were investigated.SH-SY5Y cells were treated with rotenone to establish PD cell model; the effects of rotenone and SNP-9 on cell viability were detected by MTT assay; Hoechst/PI double staining assay was used to detect the effects of rotenone and SNP-9 on cell apoptosis; DCFH-DA probe was used to detect the effects of rotenone and SNP-9 on cellular reactive oxygen species (ROS) levels; and Western blot was used to detect the effects of rotenone and SNP-9 on protein levels of tyrosine hydroxylase (TH), α-synuclein (α-syn), Bcl-2 and Bax.The results showed that SNP-9 could alleviate abnormalities in cell viability, levels of TH and α-syn, apoptosis, ROS and apoptotic relative protein Bax/Bcl-2 induced by rotenone.Our findings suggest that SNP-9 may alleviate rotenone-induced injury in neuronal cells by regulating cell apoptosis related pathway.
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    • References (21)
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