Characterization of chemical constituents of <i>Heibu Yaogao</i> (ointment)

CHENG Xiaohong; LIU Huijun; JIANG Li; LIU Lifang

doi:10.11665/j.issn.1000-5048.2023020601

Journal of China Pharmaceutical University > 2023 > 54(1): 77-88. > DOI: 10.11665/j.issn.1000-5048.2023020601

CHENG Xiaohong, LIU Huijun, JIANG Li, LIU Lifang. Characterization of chemical constituents of Heibu Yaogao (ointment)[J]. Journal of China Pharmaceutical University, 2023, 54(1): 77-88. DOI: 10.11665/j.issn.1000-5048.2023020601

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Characterization of chemical constituents of Heibu Yaogao (ointment)

1.
Hospital for Skin Diseases, Chinese Academy of Medical Sciences, Nanjing 210042
2.
Department of Pharmacognosy, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China

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Received Date: February 05, 2023
Revised Date: March 02, 2023

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Abstract

Abstract

Heibu Yaogao (ointment), which made of Galla chinensis, Scolopendra, etc., is a commonly used hospital preparation with definite clinical efficacy, yet its material basis has not been fully clarified.By ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS), the chemical constituents of Heibu Yaogao (ointment) and its crude drug mixture were qualitatively analyzed, and the differences of components before and after preparation were discussed.Scanned by electrospray ionization (ESI) source in positive and negative ion modes, a total of 73 compounds were identified via precise molecular weight, characteristic fragment ions and chromatographic retention time.57 and 65 compounds were detected in crude drug mixture and Heibu Yaogao (ointment) respectively, including tannins, phenolic acids, nucleosides, amino acids, etc.In the process of preparation, the chemical structure changes are mainly concentrated in tannins.The change of components of Heibu Yaogao (ointment) before and after processing were proved by UPLC-Q-TOF-MS/MS, which provides important reference for elucidating the material basis of Heibu Yaogao (ointment), illustrating the rationality of preparation technology and further promoting the quality control of the preparation.
- Heibu Yaogao (ointment),
- Galla chinensis,
- Scolopendra,
- UPLC-Q-TOF-MS/MS,
- chemical constituent

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References (32)

References

[1]	. Dermatol Bull（皮肤科学通报），2017，34（6）：656-661.
[2]	Cao W，Qu JH. Experience of Zhao Bing''nan Heibu ointment in treating keloid［J］. Beijing J Tradit Chin Med（北京中医药），2019，38（10）：956-958.
[3]	Xie J，Wu JK. Brief investigation of pharmacological mechanism by main components in Heibu ointment［J］. Chin J Mod Drug Appl（中国现代药物应用），2016，10（20）：271-272.
[4]	Cao GY，Geng SX，Luo Y，et al. The rapid identification of chemical constituents in Fufang Xiling Jiedu capsule，a modern Chinese medicine，by ultra-performance liquid chromatography coupled with quadrupole-time-of-flight tandem mass spectrometry and data mining strategy［J］. J Sep Sci，2021，44（9）：1815-1823.
[5]	Liu KX，Li N，Yin YH，et al. An in-house database-driven untargeted identification strategy for deep profiling of chemicalome in Chinese medicinal formula［J］. J Chromatogr A，2022，1666：462862.
[6]	Maugeri A，Lombardo GE，Cirmi S，et al. Pharmacology and toxicology of tannins［J］. Arch Toxicol，2022，96（5）：1257-1277.
[7]	Enomoto H. Unique distribution of ellagitannins in ripe strawberry fruit revealed by mass spectrometry imaging［J］. Curr Res Food Sci，2021，4：821-828.
[8]	Valanciene E，Jonuskiene I，Syrpas M，et al. Advances and prospects of phenolic acids production，biorefinery and analysis［J］. Biomolecules，2020，10（6）：874.
[9]	Akande T，Khatib M，Ola Salawu S，et al. ¹H NMR and HPLC-DAD-MS for the characterization of ellagitannins and triterpenoids of less investigated Anogeissus leiocarpus DC （Combretaceae） stem bark［J］. Food Chem，2022，375：131813.
[10]	Zhang Y，Dong WT，Huo JH，et al. Analysis on chemical constituents of Pheretima aspergillum by UPLC-Q-TOF-MS［J］. Chin Tradit Herb Drugs（中草药），2017，48（2）：252-262.
[11]	Zhang QQ，Feng Y，Li CH，et al. Rapid identification of phthalides and organic acids in Angelica sinensis based on UPLC-Q-TOF/MS［J］. China Pharm（中国药房），2022，33（5）：579-585，591.
[12]	Kiss AK，Piwowarski JP. Ellagitannins，gallotannins and their metabolites- the contribution to the anti-inflammatory effect of food products and medicinal plants［J］. Curr Med Chem，2018，25（37）：4946-4967.
[13]	Barnes RC，Krenek KA，Meibohm B，et al. Urinary metabolites from mango （Mangifera indica L. cv. Keitt） galloyl derivatives and in vitro hydrolysis of gallotannins in physiological conditions［J］. Mol Nutr Food Res，2016，60（3）：542-550.
[14]	Virtanen V，Karonen M. Partition coefficients （logP） of hydrolysable tannins［J］. Molecules，2020，25（16）：3691.
[15]	Watrelot AA，Le Guernevé C，Hallé H，et al. Multimethod approach for extensive characterization of gallnut tannin extracts［J］. J Agric Food Chem，2020，68（47）：13426-13438.
[16]	Engstr?m MT，Virtanen V，Salminen JP. Influence of the hydrolyzable tannin structure on the characteristics of insoluble hydrolyzable tannin-protein complexes［J］. J Agric Food Chem，2022，70（41）：13036-13048.
[17]	Banc R，Rusu ME，Filip L，et al. The impact of ellagitannins and their metabolites through gut microbiome on the gut health and brain wellness within the gut-brain axis［J］. Foods，2023，12（2）：270.
[18]	Chávez-González ML，Guyot S，Rodríguez-Herrera R，et al. Exploring the degradation of gallotannins catalyzed by tannase produced by Aspergillus niger GH1 for ellagic acid production in submerged and solid-state fermentation［J］. Appl Biochem Biotechnol，2018，185（2）：476-483.
[19]	He HF. Recognition of gallotannins and the physiological activities：from chemical view［J］. Front Nutr，2022，9：888892.
[20]	Chambi F，Chirinos R，Pedreschi R，et al. Antioxidant potential of hydrolyzed polyphenolic extracts from Tara （Caesalpinia spinosa） pods［J］. Ind Crops Prod，2013，47：168-175.
[21]	Kim TJ，Silva JL，Kim MK，et al. Enhanced antioxidant capacity and antimicrobial activity of tannic acid by thermal processing［J］. Food Chem，2010，118（3）：740-746.
[22]	Wang SX. Clinical observation on the treatment of hypertrophic scar with Heibu ointment（黑布药膏治疗增生性瘢痕的临床观察）［D］. Shenyang：Liaoning University of Traditional Chinese Medicine，2009.
[23]	Liang Z，Xu Q，Zhang Q，et al. Research progress on chemical constituents and pharmacological effects of Galla chinensis［J］. Chin Tradit Herb Drugs（中草药），2022，53（18）：5908-5919.
[24]	Ji LL，Lv SW，Yang ZX. Research progress of chemical constituents and pharmacological action of centipede［J］. Special Wild Econ Animal Plant Res（特产研究），2020，42（4）：75-84.
[25]	Marucci G，Buccioni M，Varlaro V，et al. The possible role of the nucleoside adenosine in countering skin aging：a review［J］. Biofactors，2022，48（5）：1027-1035.
[26]	Zhang YY，Cai SB，Ma S，et al. Water caltrop （Trapa quadrispinosa roxb.） husk improves oxidative stress and postprandial blood glucose in diabetes：phenolic profiles，antioxidant activities and α-glycosidase inhibition of different fractions with in vitro and in silico analyses［J］. Antioxidants （Basel），2022，11（10）：1873.
[27]	Xiao SS，Yu H，Xie YF，et al. The anti-inflammatory potential of Cinnamomum camphora （L.） J.Presl essential oil in vitro and in vivo［J］. J Ethnopharmacol，2021，267：113516.
[28]	Almasaudi S. The antibacterial activities of honey［J］. Saudi J Biol Sci，2021，28（4）：2188-2196.
[29]	Burian M，Wolz C，Yazdi AS. Transcriptional adaptation of staphylococci during colonization of the authentic human environment：an overview of transcriptomic changes and their relationship to physiological conditions［J］. Front Cell Infect Microbiol，2022，12：1062329.
[30]	Prasath KG，Alexpandi R，Parasuraman R，et al. Anti-inflammatory potential of myristic acid and palmitic acid synergism against systemic candidiasis in Danio rerio （Zebrafish）［J］. Biomed Pharmacother，2021，133：111043.
[31]	Saedtler M，F?rtig N，Ohlsen K，et al. Antibacterial anacardic acid derivatives［J］. ACS Infect Dis，2020，6（7）：1674-1685.
[32]	Xu C，Liu YL，Gao ZW，et al. Pharmacological activities of myricetin and its glycosides［J］. China J Chin Mater Med（中国中药杂志），2020，45（15）：3575-3583.

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