Mechanism of M2 macrophage-derived exosomes in promoting the migration of glioblastoma via transferring miR-1260b

ZHANG Heng; CHEN Qianqian; GUI Yanping; HUANG Min; GUO Yabing; ZHAO Li

doi:10.11665/j.issn.1000-5048.2023020901

Journal of China Pharmaceutical University > 2023 > 54(1): 95-106. > DOI: 10.11665/j.issn.1000-5048.2023020901

ZHANG Heng, CHEN Qianqian, GUI Yanping, HUANG Min, GUO Yabing, ZHAO Li. Mechanism of M2 macrophage-derived exosomes in promoting the migration of glioblastoma via transferring miR-1260b[J]. Journal of China Pharmaceutical University, 2023, 54(1): 95-106. DOI: 10.11665/j.issn.1000-5048.2023020901

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Mechanism of M2 macrophage-derived exosomes in promoting the migration of glioblastoma via transferring miR-1260b

1.
School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing 211198
2.
Center of Pharmaceutical Animal Experiments, China Pharmaceutical University, Nanjing 211198, China

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Received Date: February 08, 2023
Revised Date: February 22, 2023

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Abstract

Abstract

Tumor-associated macrophage promotes the progression of glioblastoma (GBM) by infiltrating into tumor tissue, yet its mechanism has not been fully elucidated.This paper aimed to investigate the mechanism of M2 macrophages in affecting the migratory capacity of GBM via secreting exosomes.Ultracentrifugation was used to extract exosomes; RNA sequencing was carried out to screen differentially expressed miRNAs; target prediction database was used to predict the possible target proteins of miRNA; Dual-luciferase reporter assay was performed to verify the interaction between miRNA and target genes; and the proliferation ability of tumor cells was detected by subcutaneous xenograft model in nude mice.Results showed that tumor-related macrophages were mainly M2 macrophages, and that exosomes secreted by M2 macrophages could promote the migration of glioma cells.Meanwhile, exosomes secreted by M2 macrophages transported miR-1260b and affected the migration of glioma cells through directly targeted AJAP1, suggesting that exosomes secreted by macrophages could affect the migration ability of GBM through transporting miR-1260b.
- glioblastoma,
- M2 macrophage,
- exosomes,
- miR-1260b,
- AJAP1

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References

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Mechanism of M2 macrophage-derived exosomes in promoting the migration of glioblastoma via transferring miR-1260b

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