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PEI Xin, LI Guideng, CHU Weihua. Progress of proximity labeling technology in membrane protein interaction[J]. J China Pharm Univ, 2024, 55(2): 158 − 166. DOI: 10.11665/j.issn.1000-5048.2023041303
Citation: PEI Xin, LI Guideng, CHU Weihua. Progress of proximity labeling technology in membrane protein interaction[J]. J China Pharm Univ, 2024, 55(2): 158 − 166. DOI: 10.11665/j.issn.1000-5048.2023041303
shu

Progress of proximity labeling technology in membrane protein interaction

  • 1.

    School of Life Science and Technology, China Pharmaceutical University, Nanjing 211198

  • 2.

    Suzhou Institute of Systems Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Suzhou 215123, China

Funds: This work was supported by the National Natural Science Foundation of China (No. 81972875，No.32270994) and the Natural Science Foundation Outstanding Youth Fund of Jiangsu Province (No. BK20211505)
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  • Received Date: April 12, 2023
    Graphical Abstract
    • Abstract

  • Membrane proteins, which play a critical role in various life processes, particularly in regulating cell-cell contact and signal transduction, are closely linked to cell differentiation and maturation. Therefore, it is of great theoretical and practical significance to develop a variety of methods to thoroughly explore the interactions between membrane proteins. In addition to traditional techniques such as immunoprecipitation, newly developed proximity labeling (PL) techniques have gradually become important means to study membrane protein interaction. PL methods are based on engineered enzymes fused with bait protein to catalyze small molecules, label neighboring target proteins, and detect the interactions by flow cytometry, mass spectrometry, confocal microscopic imaging, etc. This paper focuses on the recent developments in PL techniques for studying membrane protein interactions, with a prospect of the potential future directions for research in this area.

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