Inhibition of CoCl<sub>2</sub>-induced glycolysis in HUVEC cells by heparin-derived oligosaccharides and its mechanism

QIN Yuning; YAO Yuhao; RUBAYIZA Joshua; HE Shuying

doi:10.11665/j.issn.1000-5048.2023042501

Journal of China Pharmaceutical University > 2023 > 54(5): 626-633. > DOI: 10.11665/j.issn.1000-5048.2023042501

QIN Yuning, YAO Yuhao, RUBAYIZA Joshua, HE Shuying. Inhibition of CoCl₂-induced glycolysis in HUVEC cells by heparin-derived oligosaccharides and its mechanism[J]. Journal of China Pharmaceutical University, 2023, 54(5): 626-633. DOI: 10.11665/j.issn.1000-5048.2023042501

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Inhibition of CoCl₂-induced glycolysis in HUVEC cells by heparin-derived oligosaccharides and its mechanism

School of Life Science & Technology, China Pharmaceutical University, Nanjing 211198, China

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Received Date: April 24, 2023
Revised Date: October 17, 2023

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Abstract

In this paper, cobalt chloride was used to stimulate human umbilical vein endothelial cells (HUVEC) to establish a model of abnormal hypoxic injury, to investigate the effect of heparin-derived oligosaccharides (HDO) on glycolysis in HUVEC cells and its molecular mechanism.The experiment was divided into the control group (FBS-free DMEM medium), the model group (FBS-free DMEM medium +50 μmol/L CoCl₂), and the HDO group (modeling+0.01, 0.1, 1 μmol/L HDO).Firstly, a biochemical kit was used to detect the effects of HDO on glucose uptake and lactic acid accumulation in HUVEC cells, then Western blot and qPCR were used to detect the effects of HIF-1α, GLUT-1 and LDHA gene transcription and protein expression, and finally, PI3K/Akt signaling pathway was detected.The results showed that HDO inhibited glucose uptake and lactate production, down-regulated the expression of HIF-1α, GLUT-1, and LDHA, and affected the activation of the PI3K/Akt signaling pathway.HDO could regulate the glycolysis level of HUVEC cells by inhibiting the activation of the PI3K/Akt/HIF-1α signaling axis.
- heparin-derived oligosaccharides,
- glycolysis,
- human umbilical vein endothelial cells,
- hypoxia-inducible factor

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Inhibition of CoCl2-induced glycolysis in HUVEC cells by heparin-derived oligosaccharides and its mechanism

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Inhibition of CoCl₂-induced glycolysis in HUVEC cells by heparin-derived oligosaccharides and its mechanism