Effects of <i>Pseudomonas aeruginosa</i> PmrB<sub>△Leu172</sub> mutation on polymyxin B resistance

DENG Zhen; HUANG Wei; WANG Xiaoqin

doi:10.11665/j.issn.1000-5048.2023121001

Journal of China Pharmaceutical University > 2024 > 55(6): 809-815. > DOI: 10.11665/j.issn.1000-5048.2023121001

DENG Zhen, HUANG Wei, WANG Xiaoqin. Effects of Pseudomonas aeruginosa PmrB_△Leu172 mutation on polymyxin B resistance[J]. J China Pharm Univ, 2024, 55(6): 809 − 815. DOI: 10.11665/j.issn.1000-5048.2023121001

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Effects of Pseudomonas aeruginosa PmrB_△Leu172 mutation on polymyxin B resistance

1.
College of Pharmacy, Guangdong Medical University, Dongguan 523000
2.
Department of Clinical Laboratory of Shenzhen People's Hospital/The Second Affiliated Clinical Medical College of Jinan University/The First Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518020, China

Funds: This study was supported by the Guangdong Basic and Applied Basic Research Joint Fund Project(2022A1515140129); and the Shenzhen Science and Technology Program Project (GJHZ20220913142800001)

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Received Date: December 09, 2023

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Abstract

Abstract

This study aimed to investigate the effects of PmrB_△Leu172 on polymyxin B resistance in Pseudomonas aeruginosa. Pseudomonas aeruginosa PmrB_△Leu172 strain was constructed by homologous recombination technology, and the minimum inhibitory concentration of polymyxin B against wild type and mutant strains were determined by the broth microdilution technique. The effect of PmrB_△Leu172 mutation on gene transcription level was determined by RNA sequencing. Reverse transcription quantitative PCR was used to verify the effect of PmrB_△Leu172 mutation on the transcription level of PmrB-regulated genes in P. aeruginosa. The results showed that the minimum inhibitory concentration and minimum bactericidal concentration of mutant strain were 4 and 2 times higher than that of wild-type, respectively, and the transcription level of PmrB-regulated genes was also up-regulated. The results suggested that PmrB_△Leu172 mutation of P. aeruginosa can enhance the regulation of PmrA-PmrB two-componentsystem, which leads to the resistance of P. aeruginosa to polymyxin B.
- Pseudomonas aeruginosa,
- pmrB,
- drug resistance,
- polymyxin B

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References (17)

References

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