| Citation: |
ZHANG Xiaojun, HUANG Shanqin, GUO Xiaohan, et al. Preparation and evaluation of in situ film of compound iodine oral spray for promoting oral ulcer repair[J]. J China Pharm Univ, 2024, 55(6): 775 − 782. DOI: 10.11665/j.issn.1000-5048.2024030601
|
In this study, a new in situ film of compound iodine oral spray was prepared by in situ gel technology, which was used to exert sustained-release effect for promoting the repair of oral ulcer wounds. Firstly, the formulation and process of the spray solution were optimized according to the spray state and film-forming time. The drug-liquid mixing ratio was evaluated by film-forming time and drug film adhesion. The drug content, stability, pH, and spraying effect of compound iodine oral spray prepared by the optimal formulation were investigated; and the physicochemical properties, including film formation time, solubility, hygroscopicity, moisture retention and in vitro release of drug film were evaluated. In addition, the biocompatibility of the film-forming materials and proliferation ability of drug film were investigated by cell experiment. Through the rabbit oral ulcer model, the in vivo film-forming and repair-promoting effects of compound iodine oral spray were evaluated. The results showed that the pH of liquid A and liquid B prepared were 6.21±0.02 and 6.42±0.03, respectively, which were in line with the normal pH range of oral mucosa; liquid A and liquid B had good stability and spray state; the iodine content in solution B was (1.96±0.01) mg/mL; the in situ membrane formation time in vitro and in the oral cavity were (118.3±3.6) s and (133.3±4.6) s, respectively; the 24-hour dissolution rate was (87.31±1.74)%, the moisture absorption rate was (124.17±7.13)%, and the moisture retention rate was (26.85±2.50)%; the iodine content in the oral spray was (47.42±0.39) mg/g, with good flexibility and adhesion, as well as some slow-release effect. In cell experiment, the film-forming materials showed good biocompatibility and growth promotion ability. The results of the rabbit oral ulcer experiment showed that the compound iodine oral spray could rapidly form a film in vivo and significantly promote the repair of oral ulcer. In conclusion, the compound iodine oral spray in situ film with a stable preparation process can effectively promote the repair of oral ulcer wounds, which provides a new idea for the research of novel oral mucosa formulations, with a good prospect of transfer.
| [1] |
Zhang XL. Discussion and analysis of treatment methods and research progress of oral ulcers[J]. J Clin Med (临床医药文献杂志), 2018, 5(55): 191-192.
|
| [2] |
Li JJ, Wen ZH, Lou Y, et al. Soluble epoxide hydrolase inhibitor promotes the healing of oral ulcers[J]. Clinics, 2023, 78: 100208. doi: 10.1016/j.clinsp.2023.100208
|
| [3] |
He SY, Bai JF, Liu YH, et al. A polyglutamic acid/tannic acid-based nano drug delivery system: antibacterial, immunoregulation and sustained therapeutic strategies for oral ulcers[J]. Int J Pharm, 2023, 648: 123607. doi: 10.1016/j.ijpharm.2023.123607
|
| [4] |
Zeng YY, Gao YJ, He LM, et al. Multifunctional polysaccharide composited microneedle for oral ulcer healing[J]. Mater Today Biol, 2023, 22: 100782. doi: 10.1016/j.mtbio.2023.100782
|
| [5] |
Ge WH, Gao YJ, He LM, et al. Combination therapy using multifunctional dissolvable hyaluronic acid microneedles for oral ulcers[J]. Int J Biol Macromol, 2023, 251: 126333. doi: 10.1016/j.ijbiomac.2023.126333
|
| [6] |
Zeng X, Jin X, Zhong L, et al. Difficult and complicated oral ulceration: an expert consensus guideline for diagnosis[J]. Int J Oral Sci, 2022, 14(1): 28. doi: 10.1038/s41368-022-00178-0
|
| [7] |
Dou X, Li GT, Wang S, et al. Probiotic-loaded calcium alginate/fucoidan hydrogels for promoting oral ulcer healing[J]. Int J Biol Macromol, 2023, 244: 125273. doi: 10.1016/j.ijbiomac.2023.125273
|
| [8] |
Zhang ZJ, Zhang QY, Gao SN, et al. Antibacterial, anti-inflammatory and wet-adhesive poly(ionic liquid)-based oral patch for the treatment of oral ulcers with bacterial infection[J]. Acta Biomater, 2023, 166: 254-265. doi: 10.1016/j.actbio.2023.05.017
|
| [9] |
Kanagalingam J, Amtha R. Povidone-iodine in dental and oral health: a narrative review[J]. J Int Oral Health, 2020, 12(5): 407. doi: 10.4103/jioh.jioh_89_20
|
| [10] |
Deng QM, Wei CY, Luo JL, et al. Experimental study of Emilia sonchifolia combined with Coptidis rhizoma for the prevention and treatment of oral ulcer[J]. Open J Stomatol, 2023, 13(8): 233-242. doi: 10.4236/ojst.2023.138020
|
| [11] |
Li ZY, Liang XY, Qiu ZT, et al. Ion-interferential cell cycle arrest for melanoma treatment based on magnetocaloric bimetallic-ion sustained release hydrogel[J]. Chin Chemical Lett, 2024: 109592.
|
| [12] |
Shi M, Yong Q, He Y, et al. Construction and in vivo evaluation of a thermosensitive hydrogel system loading with Pseudomonas aeruginosa DNA vaccine[J]. J China Pharm Univ (中国药科大学学报), 2021, 52(2): 186-194.
|
| [13] |
Samiraninezhad N, Asadi K, Rezazadeh H, et al. Using chitosan, hyaluronic acid, alginate, and gelatin-based smart biological hydrogels for drug delivery in oral mucosal lesions: a review[J]. Int J Biol Macromol, 2023, 252: 126573. doi: 10.1016/j.ijbiomac.2023.126573
|
| [14] |
Sha K, Li JC, Qi XL. Application and research progress of in situ gel for local treatment of periodontitis[J]. J China Pharm Univ (中国药科大学学报), 2022, 53(3): 365-375.
|
| [15] |
Chinese Pharmacopoeia Commission. Chinese Pharmacopoeia: Part 4(中华人民共和国药典: 四部)[S]. Beijing: China Medical Science Press, 2020: 119-120.
|
| [16] |
Ni R, Ma JL, Sun YL, et al. Preparation and in vitro evaluation of a new terbinafine hydrochloride bioadhesive film-forming gel[J]. J China Pharm Univ (中国药科大学学报), 2015, 46(6): 671-676.
|
| [17] |
Wang Q. The preparation of pulsatilla radix spray film agent (白头翁喷膜剂的研制) [D]. Qingdao: Qingdao University of Science & Technology, 2019.
|
| [18] |
Qi XY, Ye YF, Chen XM, et al. Construction of targeted nanoprobe based on hyaluronic acid and its anti-tumor activity in vitro[J]. J China Pharm Univ (中国药科大学学报), 2022, 53(5): 542-553.
|
| [19] |
Liu YN. Mucoadhesive buccal film containing ornidazole and dexamethasone for oral ulcers: in vitro and in vivo studies (奥硝唑地塞米松磷酸钠口腔贴膜的制备和评价) [D]. Wuhan: Huazhong University of Science and Technology, 2019.
|
| [20] |
Du C, Liu B, Hu J, et al. Determination of iodine number of activated carbon by the method of ultraviolet–visible spectroscopy[J]. Mater Lett, 2021, 285: 129137. doi: 10.1016/j.matlet.2020.129137
|
| [21] |
Mao GZ, Tian SY, Shi YX, et al. Preparation and evaluation of a novel alginate-arginine-zinc ion hydrogel film for skin wound healing[J]. Carbohydr Polym, 2023, 311: 120757. doi: 10.1016/j.carbpol.2023.120757
|
| [22] |
Jin FY, Liao SQ, Li W, et al. Amphiphilic sodium alginate-polylysine hydrogel with high antibacterial efficiency in a wide pH range[J]. Carbohydr Polym, 2023, 299: 120195. doi: 10.1016/j.carbpol.2022.120195
|
Supported by: Beijing Renhe Information Technology Co., Ltd. 
Total visits：455393
DownLoad: