- 中国精品科技期刊
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| Citation: |
LI Junyan, LIU Tao, SUN Fang, et al. Therapeutic effect and mechanism of hordenine on ovalbumin-induced allergic rhinitis in rats[J]. J China Pharm Univ, 2025, 56(1): 80 − 90. DOI: 10.11665/j.issn.1000-5048.2024032802
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To investigate the therapeutic effect and related mechanisms of hordenine on ovalbumin (OVA)-induced allergic rhinitis (AR) in rats, HE and AB-PAS staining were used to detect the improvement of pathological damage to the nasal mucosa induced by hordenine. ELISA was employed to detect the effect of hordenine on OVA-sIgE in serum and IL-4 in the nasal mucosa supernatant of rats. IHC and Western blot experiments were undertaken to examine the effect of hordenine on Th1/Th2 cell balance. Bioinformatics analysis was performed to predict pathways, which were verified by in vivo and in vitro experiments. The experimental results showed that hordenine could alleviate the behavioral manifestations of OVA-induced AR rats, alleviate nasal mucosal pathological damage caused by AR, and reduce the secretion of OVA-sIgE and IL-4. In addition, hordenine could regulate the Th1/Th2 balance. Bioinformatics analysis results showed that the potential pathway of action of hordenine on AR was the phosphoinositide 3 kinase (PI3K)/protein kinase B (Akt) signaling pathway. The in vivo experimental results showed that the expression of PI3K and p-Akt proteins in the nasal mucosa of the model group rats was significantly increased (P < 0.01), and that the protein expression level was significantly decreased after the administration of hordenine, which was also confirmed by an in vitro experiment. This study suggests that hordenine may regulate Th1/Th2 cell balance through the PI3K/Akt signaling pathway, thereby exerting an alleviating effect on OVA-induced AR.
| [1] |
Beasley R, Worldwide variation in prevalence of symptoms of asthma, allergic rhinoconjunctivitis, and atopic eczema: ISAAC[J]. Lancet, 1998, 351 (9111): 1225-1232.
|
| [2] |
Simoneti CS, Ferraz E, de Menezes MB, et al. Allergic sensitization to laboratory animals is more associated with asthma, rhinitis, and skin symptoms than sensitization to common allergens[J]. Clin Exp Allergy, 2017, 47(11): 1436-1444. doi: 10.1111/cea.12994
|
| [3] |
Agrawal DK, Shao ZF. Pathogenesis of allergic airway inflammation[J]. Curr Allergy Asthma Rep, 2010, 10(1): 39-48. doi: 10.1007/s11882-009-0081-7
|
| [4] |
Fang DF, Zhu JF. Dynamic balance between master transcription factors determines the fates and functions of CD4 T cell and innate lymphoid cell subsets[J]. J Exp Med, 2017, 214(7): 1861-1876. doi: 10.1084/jem.20170494
|
| [5] |
Sheth K. Evaluating the safety of intranasal steroids in the treatment of allergic rhinitis[J]. Allergy Asthma Clin Immunol, 2008, 4(3): 125-129. doi: 10.1186/1710-1492-4-3-125
|
| [6] |
Braga DL, McLaughlin JL. Cactus alkaloids. V. Isolation of hordenine and N-methyltyramine from Ariocarpus retusus[J]. Planta Med, 1969, 17(1): 87-94. doi: 10.1055/s-0028-1099832
|
| [7] |
Xu ZG, Zhang QL, Ding C, et al. Beneficial effects of hordenine on a model of ulcerative colitis[J]. Molecules, 2023, 28(6): 2834. doi: 10.3390/molecules28062834
|
| [8] |
Ding FM, Wu JJ, Liu CY, et al. Effect of Xiaoyaosan on colon morphology and intestinal permeability in rats with chronic unpredictable mild stress[J]. Front Pharmacol, 2020, 11: 1069. doi: 10.3389/fphar.2020.01069
|
| [9] |
Yao J, Pan D, Zhao Y, et al. Wogonin prevents lipopolysaccharide-induced acute lung injury and inflammation in mice via peroxisome proliferator-activated receptor gamma-mediated attenuation of the nuclear factor-kappaB pathway[J]. Immunology, 2014, 143(2): 241-257. doi: 10.1111/imm.12305
|
| [10] |
Geng TX, Zang GY, Yan JC. Efficient extraction of primary peritoneal macrophages in mice induced by 3% thioglycollate broth[J]. J Jiangsu Univ(Med Ed)(江苏大学学报(医学版)), 2019, 29(1): 45-48.
|
| [11] |
Zhang QL, Wen FF, Sun F, et al. Efficacy and mechanism of quercetin in the treatment of experimental colitis using network pharmacology analysis[J]. Molecules, 2022, 28(1): 146. doi: 10.3390/molecules28010146
|
| [12] |
Yu M, Wang QM, Ma YH, et al. Aryl hydrocarbon receptor activation modulates intestinal epithelial barrier function by maintaining tight junction integrity[J]. Int J Biol Sci, 2018, 14(1): 69-77. doi: 10.7150/ijbs.22259
|
| [13] |
Sun N, Deng CR, Zhao QB, et al. Ursolic acid alleviates mucus secretion and tissue remodeling in rat model of allergic rhinitis after PM2.5 exposure[J]. Am J Rhinol Allergy, 2021, 35(2): 272-279. doi: 10.1177/1945892420953351
|
| [14] |
Li KQ, Chen Y, Jiang R, et al. Protective effects of astragaloside IV against ovalbumin-induced allergic rhinitis are mediated by T-box protein expressed in T cells/GATA-3 and forkhead box protein 3/retinoic acid-related orphan nuclear receptor γt[J]. Mol Med Rep, 2017, 16(2): 1207-1215. doi: 10.3892/mmr.2017.6685
|
| [15] |
Fu SL, Ni SH, Wang DN, et al. Coptisine suppresses mast cell degranulation and ovalbumin-induced allergic rhinitis[J]. Molecules, 2018, 23(11): 3039. doi: 10.3390/molecules23113039
|
| [16] |
Pompura SL, Dominguez-Villar M. The PI3K/Akt signaling pathway in regulatory T-cell development, stability, and function[J]. J Leukoc Biol, 2018. doi: 10.1002/JLB.2MIR0817-349R.
|
| [17] |
Wu XF, Xu R, Ouyang ZJ, et al. Beauvericin ameliorates experimental colitis by inhibiting activated T cells via downregulation of the PI3K/Akt signaling pathway[J]. PLoS One, 2013, 8(12): e83013. doi: 10.1371/journal.pone.0083013
|
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