- 中国精品科技期刊
- 中国高校百佳科技期刊
- 中国中文核心期刊
- 中国科学引文数据库核心期刊
| Citation: |
QIN Xiangying, ZHANG Liyuan, TANG Jiahui, et al. Ameliorative effect of Xuebijing injection on acute lung injury in sepsis by interfering with cGAS/STING pathway[J]. J China Pharm Univ, 2025, 56(3): 350 − 357. DOI: 10.11665/j.issn.1000-5048.2024071702
|
To investigate the effect of Xuebijing injection (XBJ) on cGAS/STING pathway in alleviating sepsis-induced acute lung injury (ALI), the mouse sepsis-induced ALI model was established by cecal ligation and puncture (CLP), and the cell inflammation model was constructed by LPS stimulating RAW264.7 cells. The effects of XBJ on lung tissue injury and cGAS/STING pathway-related protein expression in septic mice were investigated by HE staining, ELISA, and Western blot. The results showed that XBJ intervention could alleviate lung tissue injury, reduce serum IL-6, TNF-α, IFN-β, IL-1-β levels, and the expression of cGAS, STING, p-TBK1, and p-IRF3 proteins in lung tissue in vivo, and reduce the mRNA level of related inflammatory factors in RAW264.7 cells and the expression of cGAS/STING pathway proteins in vitro. The results showed that XBJ could play a role in the prevention and treatment of sepsis-induced ALI by inhibiting the inflammatory response via inhibition of the activation of cGAS/STING pathway. This study provides a new molecular mechanism for the clinical prevention and treatment of sepsis-induced acute lung injury with XBJ.
| [1] |
Yang J, Wang MX, Xu YL, et al. Discovery of 4-oxo-N-phenyl-1, 4-dihydroquinoline-3-carboxamide derivatives as novel anti-inflammatory agents for the treatment of acute lung injury and sepsis[J]. Eur J Med Chem, 2023, 249: 115144. doi: 10.1016/j.ejmech.2023.115144
|
| [2] |
Shen K, Wang XJ, Wang YW, et al. MiR-125b-5p in adipose derived stem cells exosome alleviates pulmonary microvascular endothelial cells ferroptosis via Keap1/Nrf2/GPX4 in sepsis lung injury[J]. Redox Biol, 2023, 62: 102655. doi: 10.1016/j.redox.2023.102655
|
| [3] |
Jose RJ, Manuel A. COVID-19 cytokine storm: the interplay between inflammation and coagulation[J]. Lancet Respir Med, 2020, 8(6): e46-e47. doi: 10.1016/S2213-2600(20)30216-2
|
| [4] |
Gulen MF, Samson N, Keller A, et al. cGAS-STING drives ageing-related inflammation and neurodegeneration[J]. Nature, 2023, 620(7973): 374-380. doi: 10.1038/s41586-023-06373-1
|
| [5] |
Decout A, Katz JD, Venkatraman S, et al. The cGAS-STING pathway as a therapeutic target in inflammatory diseases[J]. Nat Rev Immunol, 2021, 21(9): 548-569. doi: 10.1038/s41577-021-00524-z
|
| [6] |
Wu B, Xu MM, Fan C, et al. STING inhibitor ameliorates LPS-induced ALI by preventing vascular endothelial cells-mediated immune cells chemotaxis and adhesion[J]. Acta Pharmacol Sin, 2022, 43(8): 2055-2066. doi: 10.1038/s41401-021-00813-2
|
| [7] |
Zhao J, Zhen NX, Zhou QC, et al. NETs promote inflammatory injury by activating cGAS-STING pathway in acute lung injury[J]. Int J Mol Sci, 2023, 24(6): 5125. doi: 10.3390/ijms24065125
|
| [8] |
Zhou WA, Lai XX, Wang X, et al. Network pharmacology to explore the anti-inflammatory mechanism of Xuebijing in the treatment of sepsis[J]. Phytomedicine, 2021, 85: 153543. doi: 10.1016/j.phymed.2021.153543
|
| [9] |
Liu SQ, Yao C, Xie JF, et al. Effect of an herbal-based injection on 28-day mortality in patients with sepsis: the EXIT-SEP randomized clinical trial[J]. JAMA Intern Med, 2023, 183(7): 647-655. doi: 10.1001/jamainternmed.2023.0780
|
| [10] |
Guo J, Zhu J, Wang Q, et al. Comparative efficacy of seven kinds of Chinese medicine injections in acute lung injury and acute respiratory distress syndrome: a network meta-analysis of randomized controlled trials[J]. Front Pharmacol, 2021, 12: 627751. doi: 10.3389/fphar.2021.627751
|
| [11] |
Shang T, Zhang ZS, Wang XT, et al. Xuebijing injection inhibited neutrophil extracellular traps to reverse lung injury in sepsis mice via reducing Gasdermin D[J]. Front Pharmacol, 2022, 13: 1054176.
|
| [12] |
Pan XW, Xue LX, Zhang JZ, et al. Yiqifumai lyophilized injection attenuates sepsis-induced acute lung injury in mice[J]. J Emerg Tradit Chin Med (中国中医急症), 2021, 30(7): 1129-1133,1168.
|
| [13] |
Wang YX, Yang YY, Chen L, et al. Death-associated protein kinase 1 mediates ventilator-induced lung injury in mice by promoting alveolar epithelial cell apoptosis[J]. Anesthesiology, 2020, 133(4): 905-918. doi: 10.1097/ALN.0000000000003464
|
| [14] |
Jiao Y, Zhang T, Zhang CM, et al. Exosomal miR-30d-5p of neutrophils induces M1 macrophage polarization and primes macrophage pyroptosis in sepsis-related acute lung injury[J]. Crit Care, 2021, 25(1): 356. doi: 10.1186/s13054-021-03775-3
|
| [15] |
Yang LY, Chen HQ, Hu QY, et al. Eupalinolide B attenuates lipopolysaccharide-induced acute lung injury through inhibition of NF-κB and MAPKs signaling by targeting TAK1 protein[J]. Int Immunopharmacol, 2022, 111: 109148. doi: 10.1016/j.intimp.2022.109148
|
| [16] |
Chen JY, Huang YL, Bian XH, et al. Berberine ameliorates inflammation in acute lung injury via NF-κB/Nlrp3 signaling pathway[J]. Front Nutr, 2022, 9: 851255. doi: 10.3389/fnut.2022.851255
|
| [17] |
Sekheri M, Rizo-Téllez SA, Othman A, et al. Interferon-β regulates proresolving lipids to promote the resolution of acute airway inflammation[J]. Proc Natl Acad Sci U S A, 2022, 119(31): e2201146119. doi: 10.1073/pnas.2201146119
|
| [18] |
Shen XF, Cao K, Jiang JP, et al. Neutrophil dysregulation during sepsis: an overview and update[J]. J Cell Mol Med, 2017, 21(9): 1687-1697. doi: 10.1111/jcmm.13112
|
| [19] |
Zuo LH, Sun Z, Hu YR, et al. Rapid determination of 30 bioactive constituents in Xuebijing injection using ultra high performance liquid chromatography-high resolution hybrid quadrupole-orbitrap mass spectrometry coupled with principal component analysis[J]. J Pharm Biomed Anal, 2017, 137: 220-228. doi: 10.1016/j.jpba.2017.01.024
|
| [20] |
Li ZY, Wang B, Sun KH, et al. An aggregation-induced emission sensor combined with UHPLC-Q-TOF/MS for fast identification of anticoagulant active ingredients from traditional Chinese medicine[J]. Anal Chim Acta, 2023, 1279: 341799. doi: 10.1016/j.aca.2023.341799
|
| [21] |
Zha YF, Xie J, Ding P, et al. Senkyunolide I protect against lung injury via inhibiting formation of neutrophil extracellular trap in a murine model of cecal ligation and puncture[J]. Int Immunopharmacol, 2021, 99: 107922. doi: 10.1016/j.intimp.2021.107922
|
| [22] |
Yuan SL, Cao YW, Jiang JY, et al. Xuebijing injection and its bioactive components alleviate nephrotic syndrome by inhibiting podocyte inflammatory injury[J]. Eur J Pharm Sci, 2024, 196: 106759.
|
| [23] |
Wei JH, Liu ZJ, Sun HB, et al. Perillaldehyde ameliorates lipopolysaccharide-induced acute lung injury via suppressing the cGAS/STING signaling pathway[J]. Int Immunopharmacol, 2024, 130: 111641. doi: 10.1016/j.intimp.2024.111641
|
| [1] | ZHOU Weisai, WU Zimei, BAGULEY Bruce C., LI Caibin, ZHANG Wenli, LIU Jianping. Preparation of CI-921 mixed micelles and determination of its entrapment efficiency by method of dialysis and fitting[J]. Journal of China Pharmaceutical University, 2016, 47(5): 575-580. DOI: 10.11665/j.issn.1000-5048.20160512 |
| [2] | CHEN Juan, GUO Huihui, ZHANG Jianjun, GAO Yuan. Physicochemical characteristics of coamorphous simvastatin-gliclazide[J]. Journal of China Pharmaceutical University, 2015, 46(3): 301-308. DOI: 10.11665/j.issn.1000-5048.20150307 |
| [3] | YIN Xinjuan, YANG Yan, TIAN Yuan, TIAN Jixin, YU Pei, ZHANG Zunjian. Effect of simvastatin on the metabolism and pharmacokinetics of felodipine in rats[J]. Journal of China Pharmaceutical University, 2014, 45(4): 450-455. DOI: 10.11665/j.issn.1000-5048.20140412 |
| [4] | LIU Congyan, WANG Wei, ZHOU Jianping, DING Yang, ZHOU Xin, WANG Zhaoxian. Preparation and evaluation of gambogic acid-loaded reconstituted high density lipoprotein nanoparticles[J]. Journal of China Pharmaceutical University, 2013, 44(4): 311-315. DOI: 10.11665/j.issn.1000-5048.20130405 |
| [5] | ZHOU Li, LI Hong, YIN Li-fang, WANG Sha, CHEN Xi-jing. Preparation and pharmacokinetics of compound nicotinic acid extended-release and simvastatin immediate-release double-layer tablets[J]. Journal of China Pharmaceutical University, 2012, 43(6): 508-513. |
| [6] | SONG Yi-ting, ZHOU Hong, SUN Wan-jin, GAO Yuan, LI Chao. Preparation and physicochemical characterization of a spray-dried lornoxicam self-microemulsifying system[J]. Journal of China Pharmaceutical University, 2012, 43(2): 137-141. |
| [8] | Homocystein Inducing Monocyte Chemoattactant Protein-1 Expressed by THP-1 Macrophages and Regulative Effects by Simvastatin[J]. Journal of China Pharmaceutical University, 2003, (4): 82-84. |
| [9] | Comparative Antihyperlipidemic Effects of Simvastatin and Lovastatin[J]. Journal of China Pharmaceutical University, 2002, (5): 95-97. |
| [10] | Determination of Simvastatin in Human Plasma by LC-MS[J]. Journal of China Pharmaceutical University, 2001, (4): 44-47. |
Supported by: Beijing Renhe Information Technology Co., Ltd. 
Total visits：452654
DownLoad: