Therapeutic mechanism of of <i>Erlong Zuoci</i> Pills on oxidative stress in HEI-OC1 cells

LU Guoxia; GU Yunshuang; ZHENG Yiwen; PENG Ying; A Jiye; WANG Guangji; SUN Jianguo

doi:10.11665/j.issn.1000-5048.2024102701

Journal of China Pharmaceutical University > 2025 > 56(2): 188-195. > DOI: 10.11665/j.issn.1000-5048.2024102701

LU Guoxia, GU Yunshuang, ZHENG Yiwen, et al. Therapeutic mechanism of of Erlong Zuoci Pills on oxidative stress in HEI-OC1 cells[J]. J China Pharm Univ, 2025, 56(2): 188 − 195. DOI: 10.11665/j.issn.1000-5048.2024102701

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Therapeutic mechanism of of Erlong Zuoci Pills on oxidative stress in HEI-OC1 cells

1.
Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, China
2.
Department of Pharmacology and Toxicology, School of Biomedical Sciences, University of Otago, Dunedin 9054, New Zealand

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This study was supported by the National Key Research and Development Program for Intergovernmental Cooperation in International Science and Technology Innovation (2017YFE0109600)

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Received Date: October 26, 2024

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Abstract

Abstract

To the present study aimed to investigate the protective effects of Erlong Zuoci Pills on oxidative stress induced by hydrogen peroxide (H₂O₂) in House Ear Institute-Organ of Corti 1 (HEI-OC1) and to explore the mechanism by cellular metabolomics. There were 6 groups in the experiment: the control group, model group, three dose groups of ELZC (low, medium, and high), and positive control ascorbic acid group. The oxidative stress injury model was established in the HEI-OC1 by inducing 0.9 mmol/L H₂O₂for 12 h. The proliferation of HEI-OC1 cells was observed by CCK-8 assay; the contents and activity of lactate hydrogenase (LDH), reactive oxygen species (ROS), and superoxide dismutase (SOD) in HEI-OC1 cells were detected by corresponding kits. Finally, the endogenous substances of cells were analyzed from the perspective of metabolomics. Compared with the model group, ELZC groups could significantly increase the cell proliferation rate after administration. Moreover, they could also ameliorate the increase of ROS and LDH content and the decrease of antioxidant enzyme SOD caused by H₂O₂. Metabolomic results revealed significant differences among multiple groups in the scores of partial least squares discriminant analysis. The ELZC group could relocate the model group back to the control group. The metabolic regulation of ELZC on oxidative stress in HEI-OC1 cells mainly affects nucleotide metabolism and amino acid metabolism. In summary, the results indicate that ELZC exhibits protective effects on H₂O₂-induced oxidative stress in HEI-OC1 cells. Additionally, this protective effect may be produced by increasing the content of amino acids such as uridine and phenylalanine, thereby regulating pathways such as pyrimidine metabolism, phenylalanine metabolism, biosynthesis of phenylalanine, tyrosine, and tryptophan, and histidine metabolism.
- Erlong Zuoci Pills,
- HEI-OC1 cells,
- hydrogen peroxide,
- oxidative stress,
- metabolomics

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