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Zhao Tihui, Chen Yuying, Zhang Ji, Qian Junbin, Yu Jialin. DETERMINATION OF VITAMIN U IN TABLETS BY MICROCELLULOSE TLC SCANNING METHOD[J]. Journal of China Pharmaceutical University, 1982, (2): 10-13.
Citation: Zhao Tihui, Chen Yuying, Zhang Ji, Qian Junbin, Yu Jialin. DETERMINATION OF VITAMIN U IN TABLETS BY MICROCELLULOSE TLC SCANNING METHOD[J]. Journal of China Pharmaceutical University, 1982, (2): 10-13.
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DETERMINATION OF VITAMIN U IN TABLETS BY MICROCELLULOSE TLC SCANNING METHOD

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  • Microcellulose TLC dual wavelength TLC scanning method for determination of vitamin U [(3-amino-3-carboxypropyl) dimethyl sulfonium chloride] in tablets was employed with n-butanol-acetone 25-28% ammonia water-water ( 40:40:8:25)as developing solvent system and ninhydrin as color reagent. The R_f values of vitamin U and its decomposition product were 0.37 and 0.63 respectively.After separation cf vitamin U from its decomposition product, the dual wavelengthTLC scanning with external standard (measuring wavelength is 560nm, reference wavelength is 750 nm) was used. The recovery is 98.7% (n = 7 ).
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