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Jiang Xinru, Xu Yongming, Sun Yiping. TLCS OF VITAMINE B_6 IN WEITAXIN TABLETS[J]. Journal of China Pharmaceutical University, 1984, (3): 32-36.
Citation: Jiang Xinru, Xu Yongming, Sun Yiping. TLCS OF VITAMINE B_6 IN WEITAXIN TABLETS[J]. Journal of China Pharmaceutical University, 1984, (3): 32-36.
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TLCS OF VITAMINE B_6 IN WEITAXIN TABLETS

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  • Weitaxin tablets were ground and an acccurately weighed portion of the powder equivalent to about 25 mg of Vitamine B_6 (VB6) was dissolved in 50% MeOH to give a final vol. of 25ml which was filtered. The filtration was subjected to chromatog. on a silica gel CMC-Na plate with ethyl acetate-ether-ethanol-conc.aq. NH3 (5: 3: 3: l) and ethanol-CHC13-conc.aq.NH3(4:4:1) as two dimensional TLC solvent systems. Spots were located under UV light at 254 nm and analyzed with a CS-910 daul wavelength TLCS. The calibration graph was rectilinear in the range of 5 to 30 μg of VB6. The recovery was 100.9±0.9％. The whole procedure of analysis could be performed within hours.
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