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Determination of Warfarin Enantiomers in Serum by Capillary Electrophoresis[J]. Journal of China Pharmaceutical University, 1997, (1): 36-38.
Citation: Determination of Warfarin Enantiomers in Serum by Capillary Electrophoresis[J]. Journal of China Pharmaceutical University, 1997, (1): 36-38.
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Determination of Warfarin Enantiomers in Serum by Capillary Electrophoresis

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  • A method for the separation of warfarin enantiomers in serum by capillary electrophoresis was developed. The run buffer contained 140 mmol/L phosphate and 7 mmol /L methylated β-cyclo-dextrin(pH7.9), applied voltage 13kV, and detected at 210 nm. A baseline separation of two warfarin enantiomers can be obtained. The standard curve was linear from 0 to 5.0μg /ml and the limit ofdetection was 0.02μg/ml in serum. The precision and recovery rate was satisfying.
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