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Molecular Cloning and Expression of Tryptophanase Gene of Escherichia coli[J]. Journal of China Pharmaceutical University, 1999, (2): 61-64.
Citation: Molecular Cloning and Expression of Tryptophanase Gene of Escherichia coli[J]. Journal of China Pharmaceutical University, 1999, (2): 61-64.
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Molecular Cloning and Expression of Tryptophanase Gene of Escherichia coli

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  • kb DNA fragment was obtained from E. coli JM 105 by PCR. This fragment was inserted into NdeI/BamHI sites of pET3a. The recombinant plasmids were transformed into host strain E. coli BL 21(DE3). High level expression of tryptophanase genetic engi
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