Rapid Detection of the Mutation Point of the Human p53 Gene Exon 8 by Microchip Electrophoresis

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Journal of China Pharmaceutical University > 2003 > (4): 54-56.

Rapid Detection of the Mutation Point of the Human p53 Gene Exon 8 by Microchip Electrophoresis[J]. Journal of China Pharmaceutical University, 2003, (4): 54-56.

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Rapid Detection of the Mutation Point of the Human p53 Gene Exon 8 by Microchip Electrophoresis[J]. Journal of China Pharmaceutical University, 2003, (4): 54-56.

Citation:

Rapid Detection of the Mutation Point of the Human p53 Gene Exon 8 by Microchip Electrophoresis[J]. Journal of China Pharmaceutical University, 2003, (4): 54-56.

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Rapid Detection of the Mutation Point of the Human p53 Gene Exon 8 by Microchip Electrophoresis

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AIM:To set up a simple and rapid analytical method to detect the mutation point of gene. METHOD:Combining the modified primer Cy5 dye terminator labelled ddNTP (Cy5-ddNTP) and artificial template to do the specific Single Base Extension (SBE). Using the m
- Modified primer,
- Single base extension,
- Cy5-ddNTP,
- Microchip electrophoresis,
- Human p53 gene exon 8

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Rapid Detection of the Mutation Point of the Human p53 Gene Exon 8 by Microchip Electrophoresis

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