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QIAN Hai, HUANG Wen-long, RAO Guang-ling, GE Liang, WU Xiao-ming. Determination of 3-methyl-7，8-dihydroxy-isochroman-4-one in the peel of Musa sapientum[J]. Journal of China Pharmaceutical University, 2009, 40(6): 524-526.
Citation: QIAN Hai, HUANG Wen-long, RAO Guang-ling, GE Liang, WU Xiao-ming. Determination of 3-methyl-7，8-dihydroxy-isochroman-4-one in the peel of Musa sapientum[J]. Journal of China Pharmaceutical University, 2009, 40(6): 524-526.
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Determination of 3-methyl-7，8-dihydroxy-isochroman-4-one in the peel of Musa sapientum

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  • Aim ：To establish an HPLC method for the determination of 3-methyl-7，8-dihydroxy-isochroman-4-one in the peel of Musa sapientum. Methods ：A column of Shimadzu ODS-C18 column(250 mm×4.6 mm，5 μm)， and a mobile phase of acetonitrile-water-phosphoric acid(9∶91∶0.1) were adopted.A detect wavelength of 283 nm，a flow rate of 1 mL/min，and a column temperature of 35 °C were set. Results ：The content of 3-methyl-7，8-dihydroxy-isochroman-4-one in the peel of Musa sapientum was 0.029 5%-0.036 7%.Calibration curve was linear over the range 1.09-13.08 μg.The average recovery was 100.0%.The assay variability value was 0.64 %(n=9). Conclusion ：The method can be used for the quality control of Musa sapientum.
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