• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
Determination of monoamine neurotransmitters and related substances in mouse brain tissue by HPLC-FLD[J]. Journal of China Pharmaceutical University, 2010, 41(4): 367-371.
Citation: Determination of monoamine neurotransmitters and related substances in mouse brain tissue by HPLC-FLD[J]. Journal of China Pharmaceutical University, 2010, 41(4): 367-371.

Determination of monoamine neurotransmitters and related substances in mouse brain tissue by HPLC-FLD

More Information
  • To establish an HPLC-FLD method for the investigation of monoamine neurotransmitters and related substances in mouse frontal cortex,hippocampus,striatum,and hypothalamus.Mouse brain samples were deproteinized by perchloric acid and analyzed.The separation was carried out on a Lichrospher C18 column(250 mm×4.6 mm,5 μm).The mobile phase was composed of pH 3.06 buffer(including 1 mmol/L heptanesulfonic acid sodium salt,30 mmol/L NaH2PO4-H3PO4 solution) and methanol in the ratio of 85∶15;the flow rate was 1.0 mL/min and the column temperature was 35 °C.Fluorescence detection:the excitation and emission wavelengths were set at λex=280 nm,λem=315 nm.Under the optimized conditions,norepinephrine(NE),dopamine(DA),5-hydroxytryptamine (5-HT),3,4-dihydroxy-phenyl acetic acid(DOPAC),5-hydroxyindoleacetic acid(5-HIAA),tyrosine(Tyr),and tryptophan(Trp) in mouse brain were detected.NE,DA,and 5-HT were quantified.The linearity ranges of NE,DA, and 5-HT were 2.4-471.0 ng/mL(r=0.999 6),2.6-519.5 ng/mL (r=0.999 4),and 2.4-469.3 ng/mL(r=0.999 1),respectively.The intra- and inter-day precision was always lower than 9.0%. The accuracy could attain the demands and the absolute recovery values were always higher than 90%. The proposed method has good specificity and accuracy.It could be used to analyze the content differences of monoamine neurotransmitters and related substances in different mouse brain areas.
  • Related Articles

    [1]LI Long, GUO Wei, YU Xiuli, XIE Chunwen. Determination of the related substances in desloratadine by HPLC[J]. Journal of China Pharmaceutical University, 2019, 50(6): 707-712. DOI: 10.11665/j.issn.1000-5048.20190611
    [2]GUO Wei, XIE Chunwen, WANG Wensheng, ZHENG Deqiang, HE Shuwang. Synthesis of related substances of tebipenem pivoxil[J]. Journal of China Pharmaceutical University, 2018, 49(3): 286-290. DOI: 10.11665/j.issn.1000-5048.20180305
    [3]LUO Yan, GU Jinhua, LUO Zhiteng, WU Jie, YE Lianbao. Synthesis of the related substances of deferasirox[J]. Journal of China Pharmaceutical University, 2016, 47(3): 288-290. DOI: 10.11665/j.issn.1000-5048.20160306
    [4]ZHANG Tianshu, RUAN Zhi, LIU Xia, HUANG Jin, ZHANG Naixia. NMR-based metabolomic investigation of regional brain tissue dysfunctions of MCAO rats induced by ischemia[J]. Journal of China Pharmaceutical University, 2016, 47(2): 188-198. DOI: 10.11665/j.issn.1000-5048.20160211
    [5]YUAN Duo, ZHU Xiong, ZHANG Shijin, WANG Duo, LI Zhiyu, SUN Liping. Analysis and synthesis of the related substances of palbociclib[J]. Journal of China Pharmaceutical University, 2015, 46(6): 696-699. DOI: 10.11665/j.issn.1000-5048.20150610
    [6]YE Lianbao, WU Jieyu, YANG Jiebo, LUO Jiaoyan, HU Qiaohong. Synthesis of the related substances of gefitinib[J]. Journal of China Pharmaceutical University, 2014, 45(6): 667-670. DOI: 10.11665/j.issn.1000-5048.20140608
    [7]WANG Zhe, FENG Fang. Determination of 12 depression-related amino acids in rat plasma and brain tissue by HPLC with pre-column derivatization[J]. Journal of China Pharmaceutical University, 2012, 43(4): 345-349.
    [8]XIAO Yan-yu, PING Qi-neng. Effects of borneol-β-cyclodextrin inclusion complex on the plasma and brain concentrations of tetramethylpyrazine phosphate in mice[J]. Journal of China Pharmaceutical University, 2009, 40(5): 412-415.
    [9]Studies of Related Substances of Azithromycin[J]. Journal of China Pharmaceutical University, 2004, (1): 45-48.
    [10]Effect of SLK on Monoamine Neurotransmitter and its Metabolites in the Rat Brain[J]. Journal of China Pharmaceutical University, 1999, (5): 377-379.

Catalog

    Article views (2070) PDF downloads (2596) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return