Quality evaluation of Dendrobii Caulis by HPLC

Bibenzyls are biologically active compounds widely found in Dendrobii Caulis.A HPLC method was developed for the determination of two specific bibenzyls，moscatilin and gigantol.Chromatographic separation was performed on a reverse-phase C₁₈ column employing a binary linear gradient elution with acetonitrile and 0.01% trifluroacetic acid.The linear ranges of moscatilin and gigantol were from 2.29 μg/mL to 1 144.00 μg/mL and 1.79 μg/mL to 1 196.00 μg/mL respectively with good correlation coefficients greater than 0.999 7.The average recoveries measured at three levels were 102.7% and 99.1%，respectively.The validated method was applied for 18 different species of Dendrobium plants and 18 batches of Dendrobii Caulis，which proved to be sensitive，reliable and applicable for the quality control.