• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
XU Xiang-yang, LI Ling, WANG Qing-song, LIU Chun-hui. Preparation and in vitro evaluation of chitosan nanoparticles encapsulating recombinant human endostatin[J]. Journal of China Pharmaceutical University, 2011, 42(1): 44-47.
Citation: XU Xiang-yang, LI Ling, WANG Qing-song, LIU Chun-hui. Preparation and in vitro evaluation of chitosan nanoparticles encapsulating recombinant human endostatin[J]. Journal of China Pharmaceutical University, 2011, 42(1): 44-47.

Preparation and in vitro evaluation of chitosan nanoparticles encapsulating recombinant human endostatin

More Information
  • Chitosan nanoparticles encapsulating recombinant human endostatin (Endostar) were prepared by the ionic gelation process.Characterizations including drug loading,encapsulation efficiency,particle size,morphology,in vitro release,bioactivity and the integrity of Endostar were investigated.It was found that drug loading and encapsulation efficiency were (10.5±1.1)% and (81.3±1.8)%,respectively.The average particle diameter of the spheric nanoparticles was 137 nm.80% of cumulative release was achieved after 10 d.The results of SDS-PAGE indicated no change to the structure of Endostar during the process of preparation and in vitro release.The endothelial cell proliferation assay showed that the nanoparticles retained the bioactivity of the protein.These results indicated that Endostar nanoparticles prepared with chitosan possessed appropriate particle size and encapsulation efficiency,exhibited sustained release in vitro,maintained the structure of Endostar,and retained the bioactivity of the protein.

Catalog

    Article views (1925) PDF downloads (2218) Cited by()
    Turn off MathJax
    Article Contents

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return