Advanced Search
MENG Yu-han, LIU Su-li, ZHANG Hui-yong, CAO Rong-yue, FAN Xiao-xu, FAN Hao, LIU Jing-jing. Construction,expression and activity studies of recombinant GFP-VEGF6a fusion polypeptide[J]. Journal of China Pharmaceutical University, 2011, 42(1): 83-87.
Citation: MENG Yu-han, LIU Su-li, ZHANG Hui-yong, CAO Rong-yue, FAN Xiao-xu, FAN Hao, LIU Jing-jing. Construction,expression and activity studies of recombinant GFP-VEGF6a fusion polypeptide[J]. Journal of China Pharmaceutical University, 2011, 42(1): 83-87.
shu

Construction,expression and activity studies of recombinant GFP-VEGF6a fusion polypeptide

More Information
    Graphical Abstract
    • Abstract
  • To investigate the antitumor and anti-angiogenesis effects of vascular endothelial growth factor 6a (VEGF6a),VEGF6a and green fluorescent protein (GFP) gene were linked by GGGS segment and cloned into pET28a vector by PCR.GFP-VEGF6a (GFP6a) fusion polypeptides were expressed in E.coli and purified by heparin sepharose chromatograph.ICR mice bearing H22 hepatoma carcinoma cells was treated with subcutaneous injection of GFP6a and GFP.Compared with the negative control GFP,GFP6a (200mg/kg,20 mg/kg) significantly inhibited the growth of the peripheral blood vessels and H22 cells in vivo,with the inhibition ratios of 70.9% and 56.1% (P<0.01),respectively.VEGF6a has the potency in the application of anticancer clinical therapy.
    • FullText(HTML)
    • References (0)
    • Related Articles

  • Related Articles

    [1]LIU Jiaxuan, YAO Xueyan, TAN Yunying, HU Jing, FU Junjie, YIN Jian. Design, synthesis and anticancer activity of superoxide anion-releasing beta-galactoside prodrugs[J]. Journal of China Pharmaceutical University, 2025, 56(3): 295-304. DOI: 10.11665/j.issn.1000-5048.2024041401
    [2]BAI Zhiwei, SHANG Feiyang, DAI Weiguo, HE Liqin. Synthesis and antitumor activities of NO-donating rhein derivatives[J]. Journal of China Pharmaceutical University, 2021, 52(1): 38-43. DOI: 10.11665/j.issn.1000-5048.20210105
    [3]GU Xiaoke, TANG Xiaobo, HUANG Zhangjian, PENG Hui, ZHANG Yihua. Synthesis and antitumor activity of NO-releasing alkoxylbiphenyl derivatives[J]. Journal of China Pharmaceutical University, 2014, 45(6): 657-661. DOI: 10.11665/j.issn.1000-5048.20140606
    [4]ZHU Zi-fei, TANG Jia, WANG Ting-ting, CHEN Li. Synthesis and anti-tumor activity of novel betulinic acid derivatives[J]. Journal of China Pharmaceutical University, 2012, 43(5): 395-400.
    [5]ZHANG Shi-bo, ZHANG Zhen-zhen, DING Ye, LAI Yi-sheng, MOU Yi, AI Yong, JI Hui, ZHANG Yi-hua. Synthesis and antitumor activity of NO-donating CDDO analogues[J]. Journal of China Pharmaceutical University, 2012, 43(4): 293-297.
    [6]YANG Yong-fang, YAN Hai-qiang, DING Guo-fang, HUANG Fang-fang. Isolation and purification of an anticancer activity peptide from protein hydrolysate of Mytilus coruscus[J]. Journal of China Pharmaceutical University, 2011, 42(3): 272-275.
    [7]XU Chong, WANG Zheng-tao. Synthesis and antitumor activity of 12-[N-acylaminomethyl]-14-deoxyandrographolide derivatives[J]. Journal of China Pharmaceutical University, 2011, 42(1): 29-33.
    [9]Synthesis and Anti-inflammatory Activity of Nitric Oxidedonating α-Substituted p-(Methanesulfonyl)phenylpropenoic Acid Esters[J]. Journal of China Pharmaceutical University, 2004, (3): 6-10.
    [10]Pharmacological Activity Screening of Furoxan-coupled Diclofenac Compounds[J]. Journal of China Pharmaceutical University, 2001, (4): 63-67.

Catalog

    Get Citation
    PDF
    XML
    Article views (1772) PDF downloads (2188) Cited by()
    Turn off MathJax
    Article Contents
    Abstract
    Related Articles

    Copyright © Journal of China Pharmaceutical University苏ICP备12050101号-2

    Address：24 Tongjia Lane, Nanjing City, Jiangsu Province （210009）Tel: 025-83271566E-mail: xuebao@cpu.edu.cn

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    Total visits：452159

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return