Protective effect of recombinant hepatical stimulator analogue on concanavalina A-induced acute liver injury in mice
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Abstract
To study the effect of recombinant shark hepatical stimulator analogue (r-sHSA) on Con A-induced acute liver injury in mice,serum aminotransferase activities and histological changes of hepatocytes were measured to assess the protective effect of r-sHSA (30,60,120 μg/kg).Hepatic malondialdehyde (MDA),glutathione (GSH) and total antioxidative capacity (T-AOC) levels,serum TNF-αand IFN-γ levels,and the activity of iNOS in hepatic homogenates were measured,respectively.Within all the three treated dosages,r-sHSA exhibited significant hepatoprotective effect by decreasing mouse serum aminotransferase activities,ameliorating hepatocyte necrosis and reducing inflammatory cell infiltration.The protection mechanism might be related with the reduced hepatic oxidative stress and proinflammatory cytokine production by r-sHSA pretreatment.These results indicated that r-sHSA could protect mice from Con A-induced acute liver injury through its antioxidant and anti-inflammatory activities.
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