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Construction of a high epidaunorubicin-producing strain by gene recombination[J]. Journal of China Pharmaceutical University, 2010, 41(3): 283-288.
Citation: Construction of a high epidaunorubicin-producing strain by gene recombination[J]. Journal of China Pharmaceutical University, 2010, 41(3): 283-288.

Construction of a high epidaunorubicin-producing strain by gene recombination

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  • 4′-Epidaunorubicin is major precursor for the semisynthesis of epidoxorubicin,a clinically important antitumor antibiotic.By means of homologous recombination used for dnmV replacement and aveBⅣ integration into daunorubicin-producing strain,a mutant strain,named mHJ-02-30-1,was obtained that produces 4′-epidaunorubin.An in-frame knock-out plasmid was transformed into mHJ-02-30-1 to delete the dnrU gene fragment encoding 153 amino acids.The genetic engineering strain mYG1116E,a dnrU gene inactivation mutant,was constructed by homologous recombination,which increased the 4′-epidaunorubin production by 40% compared with the original strain.
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