• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
YANG Feng-qing, ZHANG Xue-mei, GE Li-ya, TAN Swee Ngin. Determination of nucleosides and related compounds in Ganoderma by CE-MS[J]. Journal of China Pharmaceutical University, 2011, 42(4): 337-341.
Citation: YANG Feng-qing, ZHANG Xue-mei, GE Li-ya, TAN Swee Ngin. Determination of nucleosides and related compounds in Ganoderma by CE-MS[J]. Journal of China Pharmaceutical University, 2011, 42(4): 337-341.

Determination of nucleosides and related compounds in Ganoderma by CE-MS

More Information
  • A capillary electrophoresis- mass spectrometry (CE-MS) method was established for the analysis of nucleosides and related compounds in Ganoderma lucidum and G.sinense.The separation was performed in a fused silica capillary (120 cm×50 μm) at 25 °C,with 10% methanol in water containing 100 mmol/L formic acid as CE electrolyte,and 25 kV as the applied voltage were used for the separation.The optimum MS parameters were as follows:75% methanol containing 0.3% formic acid with a flow rate of 3 μL/min was selected as the sheath liquid;the flow rate and temperature of drying gas were 6 L/min and 350 °C,respectively.ESI-MS analysis was performed in the positive mode,and the full scanning range was from m/z 50 to 350.5-Chlorocytosine arabinoside was used as the internal standard.The results showed that eight investigated compounds had good linearity (R2>0.990,except guanosine) over the tested ranges.The average recoveries were between 95.3% and 104.6%.The limit of detection (LOD) and limit of quantitation (LOQ) were 0.13-9.85 μg/mL and 0.42-20.83 μg/mL,respectively.The quantification results showed that the G.lucidum contained higher total amount of nucleosides than G.sinensis.The developed CE-MS method can be applied for the analysis of nucleosides and related compounds in Ganoderma and other traditional Chinese medicines.
  • Related Articles

    [1]CHEN Jiaquan, XU Guangfu, WANG Hui, WANG Jingjing, ZHU Fenxia. Simultaneous determination of six main compounds in Nauclea officinalis by ultra-performance liquid chromatography[J]. Journal of China Pharmaceutical University, 2014, 45(4): 434-437. DOI: 10.11665/j.issn.1000-5048.20140409
    [2]XUE Qiao-ru, LIANG Wei-yang, CHEN Yu-kun. Determination of peptide contents of hepatocyte growth-promoting factor for injection by acid hydrolytic method[J]. Journal of China Pharmaceutical University, 2011, 42(4): 333-336.
    [3]Determination of Berberine in JinQi JiangTang Tablet by HPCE[J]. Journal of China Pharmaceutical University, 2003, (4): 51-53.
    [4]Determination of Cyclosporin A in Ocular Tissues by HPLC-MS[J]. Journal of China Pharmaceutical University, 2003, (4): 44-47.
    [5]Determination of Baicalin in Scutellaria baicalensis Georgi. in the Different Parts by HPLC[J]. Journal of China Pharmaceutical University, 2001, (3): 77-78.
    [6]Determination of Contents of Dextromethorphan Hydrobromide and Guaifenesine in Capsules by HPLC[J]. Journal of China Pharmaceutical University, 1999, (5): 367-369.
    [7]Determination of in Vitro Release and Content of Dextrome thorphan Hydrobromide and Pseudoephedrine Hydrochloride Sustained-Release Tablets by HPLC[J]. Journal of China Pharmaceutical University, 1999, (5): 364-366.
    [8]Determination of Aspirin in Preparation Form by Capillary Electrophoresis[J]. Journal of China Pharmaceutical University, 1998, (2): 43-45.
    [9]Determination of Norgestrel in Compound Preparation by First Derivative Spectrography[J]. Journal of China Pharmaceutical University, 1995, (4): 246-247.
    [10]A Reversed-Phase HPLC Method for the Determination of Paeoniflorin Content[J]. Journal of China Pharmaceutical University, 1994, (4): 46-48.

Catalog

    Article views (1841) PDF downloads (2456) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return